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AB102506

Magnesium Assay Kit

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(6 Publications)

Magnesium Assay Kit (ab102506) is a simple sensitive assay for quantitating magnesium in a variety of biological samples.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Functional Studies - Magnesium Assay Kit (AB102506)
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Supplier Data

Functional Studies - Magnesium Assay Kit (AB102506)

Magnesium standard curve : Assay is performed according to kit protocol. Vertical dotted lines indicate the lower and upper limits of normal serum Magnesium concentrations.

Functional Studies - Magnesium Assay Kit (AB102506)
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Lab

Functional Studies - Magnesium Assay Kit (AB102506)

Magnesium measured in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample

Functional Studies - Magnesium Assay Kit (AB102506)
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Lab

Functional Studies - Magnesium Assay Kit (AB102506)

Colourimetric standard curve : mean of duplicates (+/-SD) with background reads substracted

Functional Studies - Magnesium Assay Kit (AB102506)
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Lab

Functional Studies - Magnesium Assay Kit (AB102506)

Magnesium measured in biological fluids showing quantity (nmol) per microliter of tested sample

Other - Magnesium Assay Kit (AB102506)
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Supplier Data

Other - Magnesium Assay Kit (AB102506)

Representative image of Magnesium Assay Kit ab102506

Components shown from left to right :

- Magnesium Standard

- Developer Solution III

- Magnesium Enzyme Mix

- Magnesium Assay Buffer

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Sample types

Urine, Plasma, Tissue Extracts, Cell culture media, Serum, Other biological fluids, Cell Lysate

Results type

Quantitative

Sensitivity

~ 60 µM

Range

2 - 15 nM

Assay time

40m

Assay Platform

Microplate reader

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Product details

Magnesium Assay Kit (ab102506) is a simple sensitive assay for quantitating magnesium in a variety of biological samples. The kit takes advantage of the specific requirement of glycerol kinase for Mg2+. An enzyme linked reaction leads to formation of an intensely colored (λmax = 450nm) product whose formation is proportional to Mg2+ concentration.

The linear range of the assay is 2-15 nmoles with detection sensitivity ~40 μM.

Other Notes
This product was previously called K385 Biovision Magnesium Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.

Magnesium is the 11th most abundant element by mass in the human body. Mg2+ is essential to all living cells where it plays an important role in facilitating the processing of biological polyphosphates like ATP, DNA, RNA and enzyme functions. Mg2+ is the metallic ion at the center of chlorophyll, and a common additive to fertilizers. Mg2+ compounds are used as laxatives, antacids, and used to stabilize abnormal nerve excitation and blood vessel spasm i.e., eclampsia.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "2000Test": { "sellingSize": "2000 Test", "publicAssetCode":"ab102506-2000Test", "assetComponentDetails": [ { "size":"20 x 100 µL", "name":"Magnesium Standard", "number":"AB102506-CMP04" }, { "size":"20 x 1 Vial", "name":"Magnesium Enzyme Mix", "number":"AB102506-CMP03" }, { "size":"20 x 25 mL", "name":"Magnesium Assay Buffer", "number":"AB102506-CMP01" }, { "size":"20 x 1 Vial", "name":"Developer Solution III", "number":"AB102506-CMP02" } ] }, "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab102506-100Test", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"Magnesium Standard", "number":"AB102506-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Magnesium Enzyme Mix", "number":"AB102506-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"Magnesium Assay Buffer", "number":"AB102506-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Solution III", "number":"AB102506-CMP02", "productcode":"AB255098" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Magnesium is an essential mineral with a significant role in numerous enzymatic reactions. It weighs approximately 24.305 atomic mass units. This mineral is expressed throughout the body particularly in bones muscles and soft tissues. The concentration of magnesium fluctuates based on dietary intake cellular demand and transport mechanisms. Magnesium ion (Mg2+) actively participates in biochemical reactions by acting as a cofactor which often stabilizes phosphate groups in ATP and other nucleotides.
Biological function summary

Magnesium contributes to cellular energy production DNA synthesis and protein synthesis. It is integral to hundreds of enzyme systems within the human body. Magnesium is not directly part of a complex but influences many enzyme systems and cellular processes. Its influence on excitability is noteworthy as it plays a central role in nerve impulse transmission and muscular contraction regulation.

Pathways

Magnesium influences several key biological pathways including the Krebs cycle and oxidative phosphorylation. Within these pathways magnesium interacts with various enzymes and proteins such as ATP synthase. Additionally magnesium plays a regulatory role in maintaining homeostasis within these systems affecting calcium and sodium ion concentrations.

Magnesium deficiency or imbalance relates to cardiovascular diseases and migraines. Cardiovascular health can be influenced by magnesium's relationship with the proteins involved in ion transport such as sodium-potassium pumps. In migraines magnesium interacts with neurotransmitter receptors and influences pathways that impact pain reception and transmission. These processes illustrate the critical importance of magnesium in maintaining health and preventing disease.
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Product protocols

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Target data

See full target information Magnesium
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in nutrition 12:1470505 PubMed40206943

2025

MgSO alleviates hippocampal neuroinflammation and BBB damage to resist CMS-induced depression.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaona Wang,Yuefeng Hu,Fan Li,Liyun Hu,Yizhu Zhang,Yunfa Qiao,Chuanfeng Tang,Renlei Wang

International journal of molecular sciences 23: PubMed36361730

2022

Coapplication of Magnesium Supplementation and Vibration Modulate Macrophage Polarization to Attenuate Sarcopenic Muscle Atrophy through PI3K/Akt/mTOR Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Can Cui,Zhengyuan Bao,Simon Kwoon-Ho Chow,Ronald Man Yeung Wong,Ailsa Welch,Ling Qin,Wing Hoi Cheung

Frontiers in microbiology 13:989421 PubMed36212840

2022

Mouse feeding study and microbiome analysis of sourdough bread for evaluation of its health effects.

Applications

Unspecified application

Species

Unspecified reactive species

Joon-Gi Kwon,Sung-Hoon Park,Jeong-Eun Kwak,Jae Hyoung Cho,Gooyoun Kim,Deukbuhm Lee,Dong Hyun Kim,Hyeun Bum Kim,Ju-Hoon Lee

Journal of clinical orthopaedics and trauma 11:S729-S734 PubMed32999547

2020

Elution properties of a resorbable magnesium phosphate cement.

Applications

Unspecified application

Species

Unspecified reactive species

Brandon L Roller,Aaron M Stoker,James L Cook

The Journal of clinical investigation 128:2226-2238 PubMed29533924

2018

Ketohexokinase C blockade ameliorates fructose-induced metabolic dysfunction in fructose-sensitive mice.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel A Lanaspa,Ana Andres-Hernando,David J Orlicky,Christina Cicerchi,Cholsoon Jang,Nanxing Li,Tamara Milagres,Masanari Kuwabara,Michael F Wempe,Joshua D Rabinowitz,Richard J Johnson,Dean R Tolan

Frontiers in neuroscience 10:561 PubMed28008305

2016

Magnesium Sulfate Prevents Placental Ischemia-Induced Increases in Brain Water Content and Cerebrospinal Fluid Cytokines in Pregnant Rats.

Applications

Unspecified application

Species

Rat

Linda W Zhang,Junie P Warrington
View all publications
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Product promise

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