Magnesium Assay Kit (ab102506) is a simple sensitive assay for quantitating magnesium in a variety of biological samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- International journal of molecular sciences 23:2022Coapplication of Magnesium Supplementation and Vibration Modulate Macrophage Polarization to Attenuate Sarcopenic Muscle Atrophy through PI3K/Akt/mTOR Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCan Cui,Zhengyuan Bao,Simon Kwoon-Ho Chow,Ronald Man Yeung Wong,Ailsa Welch,Ling Qin,Wing Hoi CheungPubMed 36361730
- Frontiers in microbiology 13:9894212022Mouse feeding study and microbiome analysis of sourdough bread for evaluation of its health effects.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoon-Gi Kwon,Sung-Hoon Park,Jeong-Eun Kwak,Jae Hyoung Cho,Gooyoun Kim,Deukbuhm Lee,Dong Hyun Kim,Hyeun Bum Kim,Ju-Hoon LeePubMed 36212840
- Journal of clinical orthopaedics and trauma 11:S729-S7342020Elution properties of a resorbable magnesium phosphate cement.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrandon L Roller,Aaron M Stoker,James L CookPubMed 32999547
- The Journal of clinical investigation 128:2226-22382018Ketohexokinase C blockade ameliorates fructose-induced metabolic dysfunction in fructose-sensitive mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiguel A Lanaspa,Ana Andres-Hernando,David J Orlicky,Christina Cicerchi,Cholsoon Jang,Nanxing Li,Tamara Milagres,Masanari Kuwabara,Michael F Wempe,Joshua D Rabinowitz,Richard J Johnson,Dean R TolanPubMed 29533924
- Frontiers in neuroscience 10:5612016Magnesium Sulfate Prevents Placental Ischemia-Induced Increases in Brain Water Content and Cerebrospinal Fluid Cytokines in Pregnant Rats.Applications:Unspecified applicationReactive species:RatLinda W Zhang,Junie P WarringtonPubMed 28008305
Key facts
- Sample types
Urine, Plasma, Tissue Extracts, Cell culture media, Serum, Other biological fluids, Cell Lysate
- Assay type
Quantitative
- Range
2 - 15 nM
- Assay time
30m
- Sensitivity
= 40 µM
Target data
What's included?
Recommended products
Magnesium Assay Kit (ab102506) is a simple sensitive assay for quantitating magnesium in a variety of biological samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Sample types
Urine, Plasma, Tissue Extracts, Cell culture media, Serum, Other biological fluids, Cell Lysate
- Assay type
Quantitative
- Range
2 - 15 nM
- Assay time
30m
- Assay Platform
Microplate reader
- Sensitivity
= 40 µM
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Magnesium Assay Kit (ab102506) is a simple sensitive assay for quantitating magnesium in a variety of biological samples. The kit takes advantage of the specific requirement of glycerol kinase for Mg2+. An enzyme linked reaction leads to formation of an intensely colored (λmax = 450nm) product whose formation is proportional to Mg2+ concentration. The linear range of the assay is 2-15 nmoles with detection sensitivity ~40 μM.
Visit our FAQs page for tips and troubleshooting.
This product is manufactured by BioVision, an Abcam company and was previously called K385 Magnesium Colorimetric Assay Kit. K385-100 is the same size as the 100 test size of ab102506.
Magnesium is the 11th most abundant element by mass in the human body. Mg2+ is essential to all living cells where it plays an important role in facilitating the processing of biological polyphosphates like ATP, DNA, RNA and enzyme functions. Mg2+ is the metallic ion at the center of chlorophyll, and a common additive to fertilizers. Mg2+ compounds are used as laxatives, antacids, and used to stabilize abnormal nerve excitation and blood vessel spasm i.e., eclampsia.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Magnesium is an essential mineral with a significant role in numerous enzymatic reactions. It weighs approximately 24.305 atomic mass units. This mineral is expressed throughout the body particularly in bones muscles and soft tissues. The concentration of magnesium fluctuates based on dietary intake cellular demand and transport mechanisms. Magnesium ion (Mg2+) actively participates in biochemical reactions by acting as a cofactor which often stabilizes phosphate groups in ATP and other nucleotides.
- Biological function summary
Magnesium contributes to cellular energy production DNA synthesis and protein synthesis. It is integral to hundreds of enzyme systems within the human body. Magnesium is not directly part of a complex but influences many enzyme systems and cellular processes. Its influence on excitability is noteworthy as it plays a central role in nerve impulse transmission and muscular contraction regulation.
- Pathways
Magnesium influences several key biological pathways including the Krebs cycle and oxidative phosphorylation. Within these pathways magnesium interacts with various enzymes and proteins such as ATP synthase. Additionally magnesium plays a regulatory role in maintaining homeostasis within these systems affecting calcium and sodium ion concentrations.
- Associated diseases and disorders
Magnesium deficiency or imbalance relates to cardiovascular diseases and migraines. Cardiovascular health can be influenced by magnesium's relationship with the proteins involved in ion transport such as sodium-potassium pumps. In migraines magnesium interacts with neurotransmitter receptors and influences pathways that impact pain reception and transmission. These processes illustrate the critical importance of magnesium in maintaining health and preventing disease.
Product promise
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4 product images
Functional Studies - Magnesium Assay Kit (ab102506)
Magnesium measured in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample
Functional Studies - Magnesium Assay Kit (ab102506)
Magnesium measured in biological fluids showing quantity (nmol) per microliter of tested sample
Functional Studies - Magnesium Assay Kit (ab102506)
Colourimetric standard curve : mean of duplicates (+/-SD) with background reads substracted
Functional Studies - Magnesium Assay Kit (ab102506)
Magnesium standard curve: Assay is performed according to kit protocol. Vertical dotted lines indicate the lower and upper limits of normal serum Magnesium concentrations.
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Product protocols
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