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AB272535

Magnesium Assay Kit

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Magnesium Assay Kit (ab272535) is a simple, direct and automation-ready procedures for measuring magnesium concentration in biological samples.
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Functional Studies - Magnesium Assay Kit (AB272535)
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Supplier Data

Functional Studies - Magnesium Assay Kit (AB272535)

Magnesium Assay Kit Standard Curve.

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Key facts

Detection method

Colorimetric

Sample types

Food samples, Urine, Milk, Serum

Assay type

Quantitative

Range

41 - 1200 µM

Assay Platform

Microplate

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Product details

Magnesium Assay Kit (ab272535) is a simple, direct and automation-ready procedures for measuring magnesium concentration in biological samples. This assay kit is designed to measure magnesium directly in biological samples without any pretreatment. A calmagite dye in the kit forms a colored complex specifically with magnesium. The intensity of the color, measured at 500 nm, is directly proportional to the magnesium concentration in the sample. The optimized formulation minimizes interference by potential substances.

Sensitive and accurate Use as little as 5 μL sample. Linear detection range 0.1 mg/dL (41μM) to 3 mg/dL (1.2 mM) Mg2+ in 96-well plate assay.

Simple and high-throughput: The procedure involves addition of two reagents and measuring OD500nm. Can be readily automated as a highthroughput assay for thousands of samples per day.

Improved reagent stability and versatility: The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay.

Low interference in biological samples: Assays can be directly performed in serum and urine samples.

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What's included?

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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Magnesium is an essential mineral with a significant role in numerous enzymatic reactions. It weighs approximately 24.305 atomic mass units. This mineral is expressed throughout the body particularly in bones muscles and soft tissues. The concentration of magnesium fluctuates based on dietary intake cellular demand and transport mechanisms. Magnesium ion (Mg2+) actively participates in biochemical reactions by acting as a cofactor which often stabilizes phosphate groups in ATP and other nucleotides.
Biological function summary

Magnesium contributes to cellular energy production DNA synthesis and protein synthesis. It is integral to hundreds of enzyme systems within the human body. Magnesium is not directly part of a complex but influences many enzyme systems and cellular processes. Its influence on excitability is noteworthy as it plays a central role in nerve impulse transmission and muscular contraction regulation.

Pathways

Magnesium influences several key biological pathways including the Krebs cycle and oxidative phosphorylation. Within these pathways magnesium interacts with various enzymes and proteins such as ATP synthase. Additionally magnesium plays a regulatory role in maintaining homeostasis within these systems affecting calcium and sodium ion concentrations.

Magnesium deficiency or imbalance relates to cardiovascular diseases and migraines. Cardiovascular health can be influenced by magnesium's relationship with the proteins involved in ion transport such as sodium-potassium pumps. In migraines magnesium interacts with neurotransmitter receptors and influences pathways that impact pain reception and transmission. These processes illustrate the critical importance of magnesium in maintaining health and preventing disease.
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Product protocols

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Target data

See full target information Magnesium
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Product promise

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