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Functional Studies - Malate Assay Kit (AB83391)
Example of Standard Curve
- Schematic Diagram
Supplier Data
Schematic Diagram - Malate Assay Kit (AB83391)
Representative image of Malate Assay Kit ab83391
Components shown from left to right :
- Malate Standar
- Developer Solution III
- Malate Enzyme Mix
- Assay Buffer 64
Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.
Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.
Product details
Abcam's Malate Assay Kit is an easy and sensitive assay to measure the L(-) Malate level in a variety of samples. In the assay, malate is specifically oxidized to generate a product which reacts with a substrate probe to generate color (λmax = 450 nm). The assay can detect 1~10 nmol of Malate in a 50 μl sample with a detection sensitivity ~20 μM.
Visit our FAQs page for tips and troubleshooting.
This product is manufactured by BioVision, an Abcam company and was previously called K637 Malate Colorimetric Assay Kit. K637-100 is the same size as the 100 test size of ab83391.
L(-) Malate is a TCA cycle intermediate. It plays an important role in the Calvin cycle during carbon fixation in plants. In lower organisms, malate is converted to lactate during malolactic fermentation with the formation of CO2. Malate is frequently used as an additive in the food and pharmaceutical industries, so quantitating malic acid is important in manufacturing beer, wine, cheese and fruits, among others.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The activity of malate is linked to energy production within cells. It is involved in the citric acid cycle converting to oxaloacetate via the enzyme malate dehydrogenase whereby NAD+ is reduced to NADH. This conversion contributes to the cycle that generates energy stored in ATP. Malate is also part of the malate-aspartate shuttle a complex that facilitates the transfer of reducing equivalents produced in glycolysis into the mitochondria. These functions enable cells to efficiently produce ATP which is essential for various cellular activities.
Pathways
The role of malate is important in bioenergetic pathways such as the citric acid cycle and gluconeogenesis. In the citric acid cycle malate’s conversion to oxaloacetate by malate dehydrogenase allows continuation of the cycle. Malate serves as a substrate for the malate-aspartate shuttle which transfers electrons across mitochondrial membranes linked to proteins such as aspartate aminotransferase. Through these pathways malate collaborates closely with proteins like NADH and FADH2 which are essential in energy production and metabolic balance within the cell.
Product protocols
- Visit the General protocols
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Target data
Publications (6)
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Journal of biochemical and molecular toxicology 38:e23854 PubMed39287333
2024
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Frontiers in oncology 12:912942 PubMed35847841
2022
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Proceedings of the National Academy of Sciences of the United States of America 118: PubMed34551979
2021
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Nature communications 10:2767 PubMed31235694
2019
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Molecular cell 69:87-99.e7 PubMed29249655
2017
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Journal of bacteriology 195:220-30 PubMed23123906
2012
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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