Malate Dehydrogenase 1 (MDH1) Activity Assay kit is designed for the sensitive and accurate measurement of MDH1 activity in human, mouse, and rat samples.
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- Acta physiologica (Oxford, England) 232:e136282021Aestivation motifs explain hypertension and muscle mass loss in mice with psoriatic skin barrier defect.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohannes Wild,Rebecca Jung,Tanja Knopp,Panagiotis Efentakis,Dimitra Benaki,Alexandra Grill,Joanna Wegner,Michael Molitor,Venkata Garlapati,Natalia Rakova,Lajos Markó,Adriana Marton,Emmanuel Mikros,Thomas Münzel,Sabine Kossmann,Manfred Rauh,Daisuke Nakano,Kento Kitada,Friedrich Luft,Ari Waisman,Philip Wenzel,Jens Titze,Susanne KarbachPubMed 33590724
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Extracts
- Assay type
- Enzyme activity (quantitative)
- Reactive species
- Mouse, Rat, Human
Target data
Function
Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH (PubMed:2449162, PubMed:3052244). Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation (PubMed:31538237). Catalyzes the reduction of 2-oxoglutarate to 2-hydroxyglutarate, leading to elevated reactive oxygen species (ROS) (PubMed:34012073).
Additional Targets
MDH1
Alternative names
MDHA, MDH1, Aromatic alpha-keto acid reductase, Cytosolic malate dehydrogenase, KAR
What's included?
Recommended products
Malate Dehydrogenase 1 (MDH1) Activity Assay kit is designed for the sensitive and accurate measurement of MDH1 activity in human, mouse, and rat samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Extracts
- Assay type
- Enzyme activity (quantitative)
- Reactive species
- Mouse, Rat, Human
- Assay Platform
- Microplate reader
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 4 | C.V. 3.4 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 4 | C.V. 8.1 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Abcam's Malate Dehydrogenase 1 (MDH1) Activity Assay kit is designed for the sensitive and accurate measurement of MDH1 activity in Human, mouse, and rat samples.
Malate Dehydrogenase 1 is the cytoplasmic isoform of the enzyme responsible for catalyzing the reversible oxidation of malate to oxaloacetate. The enzyme's activity requires the NAD+/NADH cofactor and participates primarily in the malate-aspartate shuttle.
The enzyme activity is determined by following the production of NADH in the following MDH1 catalyzed reaction:
Malate + NAD+ ↔ oxaloacetic acid + NADH
The generation of NADH is coupled to the 1:1 reduction of a reporter dye to yield a colored (yellow) reaction product whose concentration can be monitored by measuring the increase in absorbance at 450nm (Dye molar extinction coefficient: 37000M-1 cm-1). In each well, only native MDH1 is immunocaptured from the sample; this removes all other enzymes, including MDH2 from the activity measurement.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The cytosolic malate dehydrogenase commonly called MDH1 or malate dehydrogenase 1 is an enzyme that plays an important role in the citric acid cycle. Its main function is to catalyze the reversible conversion of malate to oxaloacetate using NAD+ as a cofactor. This enzyme has an approximate molecular mass of 36 kDa. MDH1 is expressed in the cytosol of various cell types throughout the body. It is critical in enabling cells to efficiently convert biochemical energy from nutrients into ATP which is vital for cellular function and survival.
- Biological function summary
This enzyme supports numerous cellular processes by maintaining redox balance and facilitating energy production. MDH1 acts independently and isn't part of any larger protein complex. However its efficiency directly impacts the cycling of metabolites necessary for cellular respiration and energy metabolism. By converting malate into oxaloacetate MDH1 directly influences processes critical for the maintenance of cell health and function.
- Pathways
MDH1 is an integral component of the tricarboxylic acid (TCA) cycle and the malate-aspartate shuttle. In the TCA cycle it plays an important role in the conversion steps that drive energy generation within cells. Additionally the malate-aspartate shuttle is an important pathway connecting cytosolic and mitochondrial processes by facilitating the exchange of reducing equivalents. Through these pathways MDH1 interacts and collaborates with proteins like aspartate aminotransferase (GOT1) and mitochondrial malate dehydrogenase (MDH2) showcasing the interconnected nature of metabolic pathways.
- Associated diseases and disorders
Dysfunction or abnormal expression of MDH1 has been associated with various conditions including cancer and metabolic syndromes. In cancer aberrant MDH1 activity can lead to altered energy metabolism promoting tumor progression and survival. Additionally MDH1's role in glucose and lipid metabolism makes it relevant in metabolic syndrome where improper energy balance can exacerbate disease states. Proteins connected through these associations include hypoxia-inducible factor 1-alpha (HIF-1α) in cancer metabolism and insulin signaling proteins in metabolic syndromes further illustrating the broader implications of MDH1's function in health and disease.
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4 product images
Functional Studies - Malate Dehydrogenase 1 (MDH1) Activity Assay (ab200009)
Raw data from HeLa cell lysate.
Once the rate/slope of each lane is extracted from the linear range of the time point data, it is expressed as rate (mOD/min) per microgram of cell lysate added per well.
Functional Studies - Malate Dehydrogenase 1 (MDH1) Activity Assay (ab200009)
Representative background-subtracted kinetic measurements from serially diluted HeLa cell lysates and tissue homogenates HLH, MLH, RLH.
Functional Studies - Malate Dehydrogenase 1 (MDH1) Activity Assay (ab200009)
MDH1 activity in cell lysates and tissue homogenates.
The assay was used to determine the MDH1 activity in a series of normal cell lysates and tissue homogenates loaded at 250 μg/mL.
Functional Studies - Malate Dehydrogenase 1 (MDH1) Activity Assay (ab200009)
MDH1 activity in serially titrated HeLa cell fractions (Cyto = Cytoplasmic, Mito = Mitochondrial and Nuc = Nuclear) demonstrates the assay's specificity to MDH1.
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