JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB112141

Maleimide Assay Kit (Fluorometric)

Be the first to review this product! Submit a review

|

(2 Publications)

Maleimide Assay Kit (Fluorometric) ab112141 uses a proprietary dye that has enhanced fluorescence upon reacting with a maleimide.
1 Images
Functional Studies - Maleimide Assay Kit (Fluorometric) (AB112141)
  • FuncS

Unknown

Functional Studies - Maleimide Assay Kit (Fluorometric) (AB112141)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted.

Key facts

Detection method

Fluorescent

Results type

Quantitative

Assay Platform

Microplate reader

style
left-column

Complementary Products

Assay Kits

AB112140

Maleimide Quantification Assay Kit (Colorimetric)

  • 0
  • 0
Assay Kits

AB112158

Free Thiol Assay Kit (Fluorometric)

Functional Studies - Free Thiol Assay Kit (Fluorometric) (AB112158)

  • 4
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Reagents, Controls & Accessories

AB286899

96-Well Black Plates

  • 0
  • 0
style
left-column

Product details

ab112141 uses a proprietary dye that has enhanced fluorescence upon reacting with a maleimide. The kit provides a sensitive, one-step fluorometric method to detect as little as 10 picomoles of maleimide in a 100 uL assay volume (100 nM; Figure 1). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be easily read by a fluorescence microplate reader at Ex/Em = 490/520 nm. Compared to ab112140, this fluorometric assay is more sensitive, and has less interference from biological samples.

Please note this kit is optimized for the detection of small molecule-based maleimide.

If your sample is maleimide conjugated in protein, then this kit may not be suitable.

ab112141 should be stored desiccated.

This product was previously called Maleimide Quantification Assay Kit (Fluorometric)

style
left-column

What's included?

{ "values": { "200Test": { "sellingSize": "200 Test", "publicAssetCode":"ab112141-200Test", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"Assay Buffer", "number":"AB112141-CMP02", "productcode":"" }, { "size":"1 x 500 µL", "name":"Reaction Buffer", "number":"AB112141-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"MalemGreen indicator", "number":"AB112141-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"DMSO", "number":"AB112141-CMP03", "productcode":"" }, { "size":"1 x 50 µL", "name":"N-ethylmaleimide Standard", "number":"AB112141-CMP04", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Maleimide also called MBS is not a protein itself but an important synthetic chemical compound commonly used in bioconjugation. Maleimide has a molecular weight of 97.09 g/mol. Scientists employ maleimide in various applications mostly within laboratory settings rather than it being naturally expressed in biological tissues. Its reactivity with thiol groups makes it an effective tool for linking molecules such as in antibody-drug conjugates.
Biological function summary

Maleimide-thiol reactions facilitate covalent linkage to thiol-containing biomolecules allowing the creation of conjugates and functionalized surfaces. Maleimide beads often functionalized with maleimide groups are used to immobilize and purify proteins. Using maleimide assay kits researchers can measure the activity and kinetics of thiol-maleimide reactions. While not a natural biological entity maleimide integrates into biomolecular complexes by bridging different biomolecules for studies.

Pathways

Protein modification by maleimide supports research in signal transduction and metabolism. For example maleimide linkage methods help explore the interactions within the MAPK signaling pathway and the ubiquitin-proteasome system. By facilitating the study of these pathways maleimide assists in dissecting protein functions and interactions with related proteins like MAP kinases and ubiquitin ligases.

Research uses maleimide to investigate cancer and neurodegenerative diseases. Its role in attaching functional tags or drugs to proteins helps in cancer therapeutics especially in targeting specific oncogenes. Additionally maleimide's utility in studying protein aggregation links it to neurodegenerative diseases such as Alzheimer's where it helps dissect the roles of proteins like tau and amyloid-beta.
style
left-column

Product protocols

style
left-column

Target data

See full target information Maleimide
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

BioTechniques 62:69-75 PubMed28193150

2017

Maleimide scavenging enhances determination of protein S-palmitoylation state in acyl-exchange methods.

Applications

Unspecified application

Species

Unspecified reactive species

Charlotte H Hurst,Dionne Turnbull,Fiona Plain,William Fuller,Piers A Hemsley

Bioconjugate chemistry 25:1971-83 PubMed25313527

2014

Peptide conjugation: before or after nanoparticle formation?

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina Valetti,Simona Mura,Magali Noiray,Silvia Arpicco,Franco Dosio,Juliette Vergnaud,Didier Desmaële,Barbara Stella,Patrick Couvreur
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com