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AB112140

Maleimide Quantification Assay Kit (Colorimetric)

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ab112140 assay kit quantifies maleimide groups by first reacting a sample with a known amount of thiol present in excess and then assaying the remaining unreacted thiol using 4,4'-DTDP with a molar extinction coefficient of 19,800 M -1cm-1.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Suspension cells, Adherent cells

Assay type

Quantitative

Sensitivity

= 10 pg/well

Assay Platform

Microplate reader

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Product details

ab112140 assay kit quantifies maleimide groups by first reacting a sample with a known amount of thiol present in excess and then assaying the remaining unreacted thiol using 4,4'-DTDP with a molar extinction coefficient of 19,800 M -1cm-1. The amount of maleimide is calculated as the difference between the initial amount of thiol and the amount of unreacted thiol after the complete reaction of all maleimide groups. This spectrophotometric assay for the determination of maleimide groups is a reverse GSH assay. It takes advantage of the high reactivity of thiols of GSH with the maleimide moiety. Maleimide of the sample is allowed to form a stable thiosuccinimidyl linkage with GSH. After the reaction of the sample is complete, the excess GSH, i.e., the remaining thiols of GSH in the reaction mixture, is estimated by using 4,4'-DTDP. The amount of GSH reacted with the sample is titrated to determine the extent of maleimide. For more sensitive maleimide quantitation, we recommend that you use our fluorimetric kit Maleimide Assay Kit (Fluorometric) ab112141 that has higher sensitivity. Visit our for tips and troubleshooting.
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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab112140-100Test", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"Assay Buffer", "number":"AB112140-CMP02", "productcode":"" }, { "size":"1 x 1 mL", "name":"DMSO", "number":"AB112140-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"4,4’-DTDP", "number":"AB112140-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"MEA", "number":"AB112140-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Maleimide also called MBS is not a protein itself but an important synthetic chemical compound commonly used in bioconjugation. Maleimide has a molecular weight of 97.09 g/mol. Scientists employ maleimide in various applications mostly within laboratory settings rather than it being naturally expressed in biological tissues. Its reactivity with thiol groups makes it an effective tool for linking molecules such as in antibody-drug conjugates.
Biological function summary

Maleimide-thiol reactions facilitate covalent linkage to thiol-containing biomolecules allowing the creation of conjugates and functionalized surfaces. Maleimide beads often functionalized with maleimide groups are used to immobilize and purify proteins. Using maleimide assay kits researchers can measure the activity and kinetics of thiol-maleimide reactions. While not a natural biological entity maleimide integrates into biomolecular complexes by bridging different biomolecules for studies.

Pathways

Protein modification by maleimide supports research in signal transduction and metabolism. For example maleimide linkage methods help explore the interactions within the MAPK signaling pathway and the ubiquitin-proteasome system. By facilitating the study of these pathways maleimide assists in dissecting protein functions and interactions with related proteins like MAP kinases and ubiquitin ligases.

Research uses maleimide to investigate cancer and neurodegenerative diseases. Its role in attaching functional tags or drugs to proteins helps in cancer therapeutics especially in targeting specific oncogenes. Additionally maleimide's utility in studying protein aggregation links it to neurodegenerative diseases such as Alzheimer's where it helps dissect the roles of proteins like tau and amyloid-beta.
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Product protocols

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Target data

See full target information Maleimide
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websiteProtocolBooklet
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