Maleimide Quantification Assay Kit (Fluorometric)

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Maleimide also called MBS is not a protein itself but an important synthetic chemical compound commonly used in bioconjugation. Maleimide has a molecular weight of 97.09 g/mol. Scientists employ maleimide in various applications mostly within laboratory settings rather than it being naturally expressed in biological tissues. Its reactivity with thiol groups makes it an effective tool for linking molecules such as in antibody-drug conjugates.

Biological function summary

Maleimide-thiol reactions facilitate covalent linkage to thiol-containing biomolecules allowing the creation of conjugates and functionalized surfaces. Maleimide beads often functionalized with maleimide groups are used to immobilize and purify proteins. Using maleimide assay kits researchers can measure the activity and kinetics of thiol-maleimide reactions. While not a natural biological entity maleimide integrates into biomolecular complexes by bridging different biomolecules for studies.

Pathways

Protein modification by maleimide supports research in signal transduction and metabolism. For example maleimide linkage methods help explore the interactions within the MAPK signaling pathway and the ubiquitin-proteasome system. By facilitating the study of these pathways maleimide assists in dissecting protein functions and interactions with related proteins like MAP kinases and ubiquitin ligases.

Associated diseases and disorders

Research uses maleimide to investigate cancer and neurodegenerative diseases. Its role in attaching functional tags or drugs to proteins helps in cancer therapeutics especially in targeting specific oncogenes. Additionally maleimide's utility in studying protein aggregation links it to neurodegenerative diseases such as Alzheimer's where it helps dissect the roles of proteins like tau and amyloid-beta.