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AB179835

Mammalian Cell Lysis Buffer 5X

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(37 Publications)

Mammalian cell lysis buffer 5X ab179835 is widely used to prepare mammalian cell and tissue lysates for various downstream biochemical assays, particularly those designed to measure enzymatic activity.

- Easy- to- use: cell lysis buffer just requires a simple 5-fold dilution
- Can also be used to lyse bacterial or plant tissues
- Cited in over 25 publications

Key facts

Sample types

Tissue, Suspension cells, Adherent cells

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Product details

Mammalian Cell Lysis Buffer 5X (ab179835) is widely used to prepare mammalian cell and tissue lysates for use in a variety of downstream biochemical assays, especially those for quantification of enzymatic activity. This buffer does not contain SDS, a reagent that interferes with many activity assays.

The lysis buffer has been optimized for mammalian samples, but it can also be used for preparation of plant or bacterial samples.

The buffer just requires a simple 5-fold dilution, and minimal hands-on-time. Cell lysates are ready in only 15 minutes.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "10mL": { "sellingSize": "10 mL", "publicAssetCode":"ab179835-10mL", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"Mammalian Cell lysis buffer 5X", "number":"AB179835-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

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Publications (37)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40362430

2025

Dysregulation of Labile Iron Predisposes Chemotherapy Resistant Cancer Cells to Ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Luke V Loftus,Louis T A Rolle,Bowen Wang,Kenneth J Pienta,Sarah R Amend

JIMD reports 65:417-425 PubMed39512436

2024

Expanding the genetic and clinical spectrum of SLC25A42-associated disorders and testing of pantothenic acid to improve CoA level in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Heckmann,Arcangela Iuso,Janine Reunert,Marianne Grüneberg,Anja Seelhöfer,Stephan Rust,Giuseppe Fiermonte,Eleonora Paradies,Carmela Piazzolla,Manoj Mannil,Thorsten Marquardt

Nature communications 15:9218 PubMed39455585

2024

Skeletal muscle reprogramming enhances reinnervation after peripheral nerve injury.

Applications

Unspecified application

Species

Unspecified reactive species

Pihu Mehrotra,James Jablonski,John Toftegaard,Yali Zhang,Shahryar Shahini,Jianmin Wang,Carey W Hung,Reilly Ellis,Gabriella Kayal,Nika Rajabian,Song Liu,Kelly C S Roballo,Susan B Udin,Stelios T Andreadis,Kirkwood E Personius

Antioxidants (Basel, Switzerland) 13: PubMed39456490

2024

Frequency-Dependent Antioxidant Responses in HT-1080 Human Fibrosarcoma Cells Exposed to Weak Radio Frequency Fields.

Applications

Unspecified application

Species

Unspecified reactive species

Hakki Gurhan,Frank Barnes

Antioxidants (Basel, Switzerland) 13: PubMed38671839

2024

Selenium Deficiency Exacerbates Hyperoxia-Induced Lung Injury in Newborn C3H/HeN Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lora C Bailey-Downs,Laura G Sherlock,Michaela N Crossley,Aristides Rivera Negron,Paul T Pierce,Shirley Wang,Hua Zhong,Cynthia Carter,Kathryn Burge,Jeffrey V Eckert,Lynette K Rogers,Peter F Vitiello,Trent E Tipple

Chemical biology & drug design 103:e14489 PubMed38404216

2024

Cinnamaldehyde protects cardiomyocytes from oxygen-glucose deprivation/reoxygenation-induced lipid peroxidation and DNA damage via activating the Nrf2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Guang Li,Jiang-Hong Li,Hai-Qin Wang,Junhua Liao,Xiao-Ya Du

Frontiers in oncology 14:1276092 PubMed38380370

2024

Diverse temporal and spatial mechanisms work, partially through Stanniocalcin-1, V-ATPase and senescence, to activate the extracellular ATP-mediated drug resistance in human cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyun Zhang,Jingwen Song,Ryan Ward,Yong Han,Arabella Hunt,Pratik Shriwas,Alexander Steed,Cory Edwards,Yanyang Cao,Milo Co,Xiaozhuo Chen

Redox biology 68:102958 PubMed37948927

2023

A role for the cystathionine-β-synthase /HS axis in astrocyte dysfunction in the aging brain.

Applications

Unspecified application

Species

Unspecified reactive species

Anindya Dey,Pijush Kanti Pramanik,Shailendra Kumar Dhar Dwivedi,Fiifi Neizer-Ashun,Tamas Kiss,Abhrajit Ganguly,Heather Rice,Priyabrata Mukherjee,Chao Xu,Mohiuddin Ahmad,Anna Csiszar,Resham Bhattacharya

Epilepsia 64:3354-3364 PubMed37777821

2023

Placental disposition of cannabidiol: An ex vivo perfusion study.

Applications

Unspecified application

Species

Unspecified reactive species

Erez Berman,Natalia Erenburg,Ron Beloosesky,Sara Eyal,Michal Kovo

Molecular psychiatry 28:2382-2397 PubMed36991133

2023

Elevated ROS levels during the early development of Angelman syndrome alter the apoptotic capacity of the developing neural precursor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lilach Simchi,Pooja Kri Gupta,Yonatan Feuermann,Hanoch Kaphzan
View all publications
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Product promise

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