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AB112142

MDR Assay Kit (Fluorometric)

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(7 Publications)

MDR Assay Kit ab112142 uses a fluorescent MDR indicator for assaying MDR pump activity. The hydrophobic fluorescent dye molecule rapidly penetrates cell membranes and becomes trapped after action by cellular enzymes. MDR transporters will expel the dye from the cell, decreasing the cellular fluorescence intensity. Readout is by fluorometric plate reader (Ex/Em 490/525 nm).

View Alternative Names

CD243, MDR1, PGY1, ABCB1, ATP-dependent translocase ABCB1, ATP-binding cassette sub-family B member 1, Multidrug resistance protein 1, P-glycoprotein 1, Phospholipid transporter ABCB1

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Functional Studies - MDR Assay Kit (Fluorometric) (AB112142)
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Supplier Data

Functional Studies - MDR Assay Kit (Fluorometric) (AB112142)

Effect of Cyclosporin A on the inhibition of P-gp pump in MCF7/ADR cells. The increased concentration of Cyclosporin A resulted in an increase in fluorescence signal caused by the inhibition of P-gp pump which enhanced the intracellular accumulation of MDR indicator dye. The EC50 = 2.4 µM (measured with the kit) is similar to the value reported in the literature.

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay type

Quantitative

Assay time

1h

Assay Platform

Microplate reader

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Product details

Multi drug resistance (MDR) is a major factor in the failure of many forms of chemotherapy. In the past few years it has become widely accepted that the resistance to chemotherapy correlates with the overexpression of at least two ATP dependent drug efflux pumps. These cell membrane proteins, called P-glycoprotein (Pgp, MDR1), and multidrug resistance associated protein (MRP1) are members of the ABC transporter family. Abcam's Fluorimetric MDR Assay Kits use a fluorescent MDR indicator for assaying these two MDR pump activities. This hydrophobic fluorescent dye molecule rapidly penetrates cell membranes and becomes trapped in cells. Following a short incubation, the intracellular free dye concentration can increase significantly. In the MDR1 and/or MRP1-expressing cells this dye is extruded by the MDR transporters, thus decreasing the cellular fluorescence intensity. However, when its extrusion is blocked by an agent that interferes with the MDR1 and/or MRP1 pump-activity, its cellular fluorescence intensity increases significantly. Abcam's Fluorimetric MDR Assay Kits are ideal for high throughput screening of MDR pump inhibitors or identifying the cells that have high level of MDR pump activities.

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Store ab112142 dessicated.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MDR also known as Multidrug Resistance protein 1 or P-glycoprotein (P-gp) is an ATP-dependent efflux pump with a molecular weight of approximately 170 kDa. This protein is integral to the plasma membrane and is expressed in various tissues including the liver kidney intestine and blood-brain barrier. MDR1 serves as a critical part of the body's defense mechanism actively exporting a wide range of substrates including drugs and toxins out of cells thereby influencing drug absorption and disposition.
Biological function summary

MDR1 plays a major role in protecting tissues by limiting drug penetration and facilitating the excretion of xenobiotics. It is part of the ATP-binding cassette (ABC) transporter family which forms a complex infrastructure of proteins responsible for the transport of molecules across cellular membranes. Its ability to pump out chemotherapy drugs significantly impacts treatment efficacy especially in certain cancer types where overexpression can lead to multidrug resistance.

Pathways

MDR1 interacts closely within the drug metabolism and transport pathways. It plays a vital role in the pharmacokinetic properties of drugs affecting both their distribution and elimination processes. MDR1 works in concert with other proteins in the ABC transporter family like MRP1 and BCRP which also contribute to drug resistance and excretion. The balance and activity of these transporters directly influence therapeutic outcomes and toxicity of cancer chemotherapeutic agents.

MDR1 is well recognized for its association with cancer particularly in its role in developing resistance to chemotherapy. Its overexpression is also noted in disorders involving drug resistance such as epilepsy where it affects the brain's protective barriers potentially limiting antiepileptic drug access. Together with proteins like LRP and BCRP MDR1 contributes to the complexity of drug delivery and efficacy in these conditions challenging clinical treatments and necessitating specific MDR assay kits for effective monitoring and management.
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Product protocols

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Target data

Translocates drugs and phospholipids across the membrane (PubMed : 2897240, PubMed : 35970996, PubMed : 8898203, PubMed : 9038218). Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins (PubMed : 8898203). Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells (PubMed : 2897240, PubMed : 35970996, PubMed : 9038218).
See full target information ABCB1

Additional targets

ABCC1
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Molecular oncology 18:386-414 PubMed37842807

2023

Repositioning VU-0365114 as a novel microtubule-destabilizing agent for treating cancer and overcoming drug resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Yao-Yu Hsieh,Jia-Ling Du,Pei-Ming Yang

Pharmaceutics 12: PubMed32545588

2020

Effect of Extract, a Herbal Drug, on the Absorption of Fexofenadine.

Applications

Unspecified application

Species

Unspecified reactive species

Jung Hwan Ahn,Junhyeong Kim,Naveed Ur Rehman,Hye-Jin Kim,Mi-Jeong Ahn,Hye Jin Chung

ACS nano 14:6947-6955 PubMed32383849

2020

Perimitochondrial Enzymatic Self-Assembly for Selective Targeting the Mitochondria of Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hongjian He,Xinyi Lin,Jiaqi Guo,Jiaqing Wang,Bing Xu

RSC advances 9:5362-5376 PubMed35515894

2019

Cisplatin, glutathione and the third wheel: a copper-(1,10-phenanthroline) complex modulates cisplatin-GSH interactions from antagonism to synergism in cancer cells resistant to cisplatin.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Vascellari,Elisa Valletta,Daniela Perra,Elisabetta Pinna,Alessandra Serra,Francesco Isaia,Alessandra Pani,Tiziana Pivetta

Oncotarget 8:87860-87877 PubMed29152126

2017

Extracellular ATP, as an energy and phosphorylating molecule, induces different types of drug resistances in cancer cells through ATP internalization and intracellular ATP level increase.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Wang,Yunsheng Li,Yanrong Qian,Yanyang Cao,Pratik Shriwas,Haiyun Zhang,Xiaozhuo Chen

Scientific reports 7:8419 PubMed28827665

2017

Schisandrin B reverses doxorubicin resistance through inhibiting P-glycoprotein and promoting proteasome-mediated degradation of survivin.

Applications

Unspecified application

Species

Unspecified reactive species

Shengpeng Wang,Anqi Wang,Min Shao,Ligen Lin,Peng Li,Yitao Wang

PloS one 9:e97512 PubMed24830744

2014

Evodiamine synergizes with doxorubicin in the treatment of chemoresistant human breast cancer without inhibiting P-glycoprotein.

Applications

FuncS

Species

Unspecified reactive species

Shengpeng Wang,Lu Wang,Zhi Shi,Zhangfeng Zhong,Meiwan Chen,Yitao Wang
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