Membrane Fluidity Assay Kit ab189819 uses a fluorescent lipophilic pyrene probe that forms excimers on spatial interaction, this shifts its emission from Em 372 nm to Em 470 nm. Measuring the ratio of 372 nm to 470 nm fluorescence gives a quantitative measure of membrane fluidity.
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- European heart journal open 3:oead0132023Hyperlipidaemia elicits an atypical, T helper 1-like CD4 T-cell response: a key role for very low-density lipoprotein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBram W van Os,Winnie G Vos,Laura A Bosmans,Claudia M van Tiel,Sanne C Lith,Myrthe S den Toom,Linda Beckers,Johannes H M Levels,Suzanne A E van Wouw,Noam Zelcer,Esther A Zaal,Celia R Berkers,Chris H A van der Lest,J Bernd Helms,Christian Weber,Dorothee Atzler,Menno P J de Winther,Jeroen Baardman,Esther LutgensPubMed 36969380
- Nature communications 14:6382023The p97-UBXD8 complex regulates ER-Mitochondria contact sites by altering membrane lipid saturation and composition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRakesh Ganji,Joao A Paulo,Yuecheng Xi,Ian Kline,Jiang Zhu,Christoph S Clemen,Conrad C Weihl,John G Purdy,Steve P Gygi,Malavika RamanPubMed 36746962
- Journal of lipid research 64:1003252023Sterol-regulated transmembrane protein TMEM86a couples LXR signaling to regulation of lysoplasmalogens in macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSuzanne A E van Wouw,Marlene van den Berg,Maroua El Ouraoui,Amber Meurs,Jenina Kingma,Roelof Ottenhoff,Melanie Loix,Marten A Hoeksema,Koen Prange,Gerard Pasterkamp,Jerome J A Hendriks,Jeroen F J Bogie,Jan B van Klinken,Frederic M Vaz,Aldo Jongejan,Menno P J de Winther,Noam ZelcerPubMed 36592658
- The New phytologist 237:930-9432022Membrane component ergosterol builds a platform for promoting effector secretion and virulence in Magnaporthe oryzae.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZiqian Guo,Xinyu Liu,Nian Wang,Pengcheng Mo,Ju Shen,Muxing Liu,Haifeng Zhang,Ping Wang,Zhengguang ZhangPubMed 36300785
- Cell reports 40:1113042022BET inhibition induces vulnerability to MCL1 targeting through upregulation of fatty acid synthesis pathway in breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGonghong Yan,Augustin Luna,Heping Wang,Behnaz Bozorgui,Xubin Li,Maga Sanchez,Zeynep Dereli,Nermin Kahraman,Goknur Kara,Xiaohua Chen,Caishang Zheng,Daniel McGrail,Nidhi Sahni,Yiling Lu,Ozgun Babur,Murat Cokol,Bora Lim,Bulent Ozpolat,Chris Sander,Gordon B Mills,Anil KorkutPubMed 36103824
- Acta pharmacologica Sinica 44:670-6792022Involvement of cell shape and lipid metabolism in glioblastoma resistance to temozolomide.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMunki Choo,Van-Hieu Mai,Han Sun Kim,Dong-Hwa Kim,Ja-Lok Ku,Sang Kook Lee,Chul-Kee Park,Yong Jin An,Sunghyouk ParkPubMed 36100765
- International journal of molecular sciences 23:2022Zinc Status Impacts the Epidermal Growth Factor Receptor and Downstream Protein Expression in A549 Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmily Scheiermann,Mary-Ann Puppa,Lothar Rink,Inga WesselsPubMed 35216384
- Nature communications 13:5122022Microskeletal stiffness promotes aortic aneurysm by sustaining pathological vascular smooth muscle cell mechanosensation via Piezo1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeiyi Qian,Tarik Hadi,Michele Silvestro,Xiao Ma,Cristobal F Rivera,Apratim Bajpai,Rui Li,Zijing Zhang,Hengdong Qu,Rayan Sleiman Tellaoui,Annanina Corsica,Ariadne L Zias,Karan Garg,Thomas Maldonado,Bhama Ramkhelawon,Weiqiang ChenPubMed 35082286
- Frontiers in cell and developmental biology 9:7969602021Differentiation of Human iPS Cells Into Sensory Neurons Exhibits Developmental Stage-Specific Cryopreservation Challenges.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Li,Patrick Walsh,Vincent Truong,Ashley Petersen,James R Dutton,Allison HubelPubMed 34970550
- American journal of physiology. Cell physiology 321:C535-C5482021Increased endothelial sodium channel activity by extracellular vesicles in human aortic endothelial cells: putative role of MLP1 and bioactive lipids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohammad-Zaman Nouri,Ling Yu,Lauren P Liu,Kevin M Chacko,Nancy D Denslow,John F LaDisa,Abdel A AlliPubMed 34288724
Key facts
- Detection method
- Fluorescent
What's included?
Recommended products
Membrane Fluidity Assay Kit ab189819 uses a fluorescent lipophilic pyrene probe that forms excimers on spatial interaction, this shifts its emission from Em 372 nm to Em 470 nm. Measuring the ratio of 372 nm to 470 nm fluorescence gives a quantitative measure of membrane fluidity.
Key facts
- Detection method
- Fluorescent
- Assay Platform
- Flow cytometer, Fluorescence microscope
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Membrane fluidity or "membrane viscosity" for short range lateral diffusion has best been measured using lipid analog probes that, when interacting, exhibit changes in their spectral properties. One of the best systems for use in such studies are the lipophilic pyrene probes that undergo excimer formation upon spatial interaction. When excimers form, the emission spectrum of the pyrene probe shifts dramatically to the red (longer wavelength). By measuring the ratio of monomer (EM max. 372 nm) to excimer (EM 470 nm) fluorescence, a quantitative monitoring of the membrane fluidity can be attained. These measurements can provide kinetic information, as well as in vivo monitoring of cellular function by both flow cytometry and microscopic analysis.
Exitation wavelength (nm): 350
Emission wavelength (nm): 450
The dynamic properties of the cell membrane and cytoplasmic microtubules and microfilaments, as well as the dynamic movement of lipids in micelles and vesicles is of importance in such diverse areas as activation of polymorphonuclear leukocytes and chemotaxis, activation of membrane enzyme systems and the specific assembly or mobilization of microtubules and microfilaments, enhancement of the affinity of chemoattractant receptors, as well as being associated with a variety of pathological syndromes related to membrane fluidity.
It has been recognized that the rotational mobility of fluorescent or magnetic resonant probes is different from that observed in lateral diffusion.
Kit size: 100 tests in a 50 μL reaction volume.
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3 product images
Fluorescent Cell Imaging - Membrane fluidity kit (ab189819)
Membrane Fluidity Kit.
Ethanol treatment increases membrane fluidity in HeLa cells
HeLa cells were treated with 100mM or 200 mM Ethanol for 48 hours, followed by 1 hour incubation with 2 μM PDA and 0.08% pluronic F-127. PDA fluorescence was monitored by exciting at 350 nM and taking emission values at 400nM (monomer) and 470nM (excimer). Relative membrane fluidity is a ratio of excimer to monomer fluorescence. Aliphatic alcohols such as EtOH are known to increase membrane fluidity.
Fluorescent Cell Imaging - Membrane fluidity kit (ab189819)
Membrane Fluidity Kit.
Isopropanol treatment increases membrane fluidity in primary leukocytes
Primary leukocytes were treated with .5% isopropanol for 1 hour, followed by 1 hour incubation with 5 μM PDA and 0.08% pluronic F-127. PDA fluorescence was measured by exciting at 350 nM and taking emission values at 400nM (monomer) and 460nM (excimer). Relative membrane fluidity is a ratio of excimer to monomer fluorescence.
Fluorescent Cell Imaging - Membrane fluidity kit (ab189819)
Membrane Fluidity Kit.
Gaucher Disease leukocytes exhibit decreased membrane fluidity relative to healthy controls
Immortalized leukocytes were incubated for 1 hour incubation with 2 μM PDA and 0.08% pluronic F-127. PDA fluorescence was measured by exciting at 350nM and taking emission values at 400nM (monomer) and 470nM (excimer). Relative membrane fluidity is a ratio of excimer to monomer fluorescence. Previous studies have demonstrated that cells isolated from Gaucher Disease patients exhibit decreased membrane fluidity.
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