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AB234041

Methionine Assay Kit (Fluorometric)

3

(1 Review)

|

(5 Publications)

Methionine Assay Kit (Fluorometric) (ab234041) uses an enzymatic mechanism by which metabolism of methionine is coupled with the stoichiometric generation of hydrogen peroxide.

View Alternative Names

Met, Methionine

3 Images
Functional Studies - Methionine Assay Kit (Fluorometric) (AB234041)
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Supplier Data

Functional Studies - Methionine Assay Kit (Fluorometric) (AB234041)

Methionine in human plasma.

The assay was run on various volumes of treated plasma and the results averaged. Methionine concentration was determined to be 18.6 μM in human plasma (2-12 μL; undiluted).

Functional Studies - Methionine Assay Kit (Fluorometric) (AB234041)
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Supplier Data

Functional Studies - Methionine Assay Kit (Fluorometric) (AB234041)

Specificity of the assay.

The assay was run in the presence of 1 nmole (1000 pmole) each of the amino acids Methionine, Cysteine, Homocysteine and Alanine. Numbers are relative to 100% response to methionine.

Functional Studies - Methionine Assay Kit (Fluorometric) (AB234041)
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Supplier Data

Functional Studies - Methionine Assay Kit (Fluorometric) (AB234041)

Example Standard Curve

Key facts

Detection method

Fluorescent

Sample types

Plasma, Serum, Cell Lysate

Assay type

Quantitative

Assay Platform

Microplate reader

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Product details

Methionine Assay Kit (Fluorometric) (ab234041) utilizes an enzymatic mechanism by which metabolism of methionine is coupled with the stoichiometric generation of hydrogen peroxide. A probe generates a fluorometric signal that can be quantified at Ex/Em: 535/587 nm. The assay shows greater than 10-fold specificity for methionine over cysteine and greater than 40-fold selectivity over other standard and non-standard amino acids. After utilization of a proprietary Sample Clean-Up Mix, the method is suitable for use in blood samples, and can detect concentrations as low as 0.5 uM, or 25 pmole Methionine.

Other Notes
This product was previously called K442 Methionine Assay Kit (Fluorometric). Biovision was acquired by Abcam in 2021.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab234041-100Test", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"Developer Mix C", "number":"AB234041-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"Met Enzyme Mix I", "number":"AB234041-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Sample Clean-Up Mix", "number":"AB234041-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Buffer Supplement I", "number":"AB234041-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Mix A", "number":"AB234041-CMP06", "productcode":"" }, { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB234041-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 45", "number":"AB234041-CMP02", "productcode":"" }, { "size":"1 x 100 µL", "name":"Methionine Standard", "number":"AB234041-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Please refer to protocols
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Product protocols

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Target data

See full target information Methionine
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:333 PubMed39747873

2025

The protein circPETH-147aa regulates metabolic reprogramming in hepatocellular carcinoma cells to remodel immunosuppressive microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Lan,Fengwei Gao,Yunshi Cai,Yinghao Lv,Jiang Zhu,Hu Liu,Sinan Xie,Haifeng Wan,Haorong He,Kunlin Xie,Chang Liu,Hong Wu

Cell reports. Medicine 5:101449 PubMed38508141

2024

Intervention with metabolites emulating endogenous cell transitions accelerates muscle regeneration in young and aged mice.

Applications

Unspecified application

Species

Unspecified reactive species

Reyna Hernandez-Benitez,Chao Wang,Lei Shi,Yasuo Ouchi,Cuiqing Zhong,Tomoaki Hishida,Hsin-Kai Liao,Eric A Magill,Sebastian Memczak,Rupa D Soligalla,Chiara Fresia,Fumiyuki Hatanaka,Veronica Lamas,Isabel Guillen,Sanjeeb Sahu,Mako Yamamoto,Yanjiao Shao,Alain Aguirre-Vazquez,Estrella Nuñez Delicado,Pedro Guillen,Concepcion Rodriguez Esteban,Jing Qu,Pradeep Reddy,Steve Horvath,Guang-Hui Liu,Pierre Magistretti,Juan Carlos Izpisua Belmonte

Nature cell biology 26:72-85 PubMed38168768

2024

Vitamin B produced by gut bacteria modulates cholinergic signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Woo Kyu Kang,Jeremy T Florman,Antonia Araya,Bennett W Fox,Andrea Thackeray,Frank C Schroeder,Albertha J M Walhout,Mark J Alkema

Nature communications 14:4758 PubMed37553341

2023

Intermittent dietary methionine deprivation facilitates tumoral ferroptosis and synergizes with checkpoint blockade.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Xue,Fujia Lu,Zhenzhen Chang,Jing Li,Yuan Gao,Jie Zhou,Ying Luo,Yongfeng Lai,Siyuan Cao,Xiaoxiao Li,Yuhan Zhou,Yan Li,Zheng Tan,Xiang Cheng,Xiong Li,Jing Chen,Weimin Wang

Nature 585:277-282 PubMed32879489

2020

Cancer SLC43A2 alters T cell methionine metabolism and histone methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yingjie Bian,Wei Li,Daniel M Kremer,Peter Sajjakulnukit,Shasha Li,Joel Crespo,Zeribe C Nwosu,Li Zhang,Arkadiusz Czerwonka,Anna Pawłowska,Houjun Xia,Jing Li,Peng Liao,Jiali Yu,Linda Vatan,Wojciech Szeliga,Shuang Wei,Sara Grove,J Rebecca Liu,Karen McLean,Marcin Cieslik,Arul M Chinnaiyan,Witold Zgodziński,Grzegorz Wallner,Iwona Wertel,Karolina Okła,Ilona Kryczek,Costas A Lyssiotis,Weiping Zou
View all publications
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