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Methionine Assay Kit (Fluorometric) (ab234041) uses an enzymatic mechanism by which metabolism of methionine is coupled with the stoichiometric generation of hydrogen peroxide.


Images

Functional Studies - Methionine Assay Kit (Fluorometric) (AB234041), expandable thumbnail
  • Functional Studies - Methionine Assay Kit (Fluorometric) (AB234041), expandable thumbnail
  • Functional Studies - Methionine Assay Kit (Fluorometric) (AB234041), expandable thumbnail

Publications

Key facts

Detection method

Fluorescent

Sample types

Plasma, Serum, Cell Lysate

Assay type

Quantitative

Target data

Alternative names

Met, Methionine

What's included?

100 Test
Components
Assay Buffer XLV
1 x 25 mL
Buffer Supplement I
1 x 1 Vial
Development Enzyme Mix I
1 x 1 Vial
Development Enzyme Mix III
1 x 200 µL
Met Enzyme Mix I
1 x 200 µL
Methionine Standard
1 x 100 µL
OxiRed Probe
1 x 200 µL
Sample Clean-Up Mix
1 x 1 Vial

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Methionine Assay Kit (Fluorometric) (ab234041) uses an enzymatic mechanism by which metabolism of methionine is coupled with the stoichiometric generation of hydrogen peroxide.

Alternative names

Met, Methionine

Key facts

Detection method

Fluorescent

Sample types

Plasma, Serum, Cell Lysate

Assay type

Quantitative

Assay Platform

Microplate reader

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

Methionine Assay Kit (Fluorometric) (ab234041) utilizes an enzymatic mechanism by which metabolism of methionine is coupled with the stoichiometric generation of hydrogen peroxide. A probe generates a fluorometric signal that can be quantified at Ex/Em: 535/587 nm. The assay shows greater than 10-fold specificity for methionine over cysteine and greater than 40-fold selectivity over other standard and non-standard amino acids. After utilization of a proprietary Sample Clean-Up Mix, the method is suitable for use in blood samples, and can detect concentrations as low as 0.5 μM, or 25 pmole Methionine.

This product is manufactured by BioVision, an Abcam company and was previously called K442 Methionine Assay Kit (Fluorometric). K442-100 is the same size as the 100 test size of ab234041.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Functional Studies - Methionine Assay Kit (Fluorometric) (ab234041), expandable thumbnail

    Functional Studies - Methionine Assay Kit (Fluorometric) (ab234041)

  • Functional Studies - Methionine Assay Kit (Fluorometric) (ab234041), expandable thumbnail

    Functional Studies - Methionine Assay Kit (Fluorometric) (ab234041)

    Methionine in human plasma.

    The assay was run on various volumes of treated plasma and the results averaged. Methionine concentration was determined to be 18.6 μM in human plasma (2-12 μL; undiluted).

  • Functional Studies - Methionine Assay Kit (Fluorometric) (ab234041), expandable thumbnail

    Functional Studies - Methionine Assay Kit (Fluorometric) (ab234041)

    Specificity of the assay.

    The assay was run in the presence of 1 nmole (1000 pmole) each of the amino acids Methionine, Cysteine, Homocysteine and Alanine. Numbers are relative to 100% response to methionine.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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