Methylated DNA Quantification Kit (Colorimetric)
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(38 Publications)
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Functional Studies - Methylated DNA Quantification Kit (Colorimetric) (AB117128)
Demonstration of high sensitivity and specificity of methylated DNA detection achieved using ab117128. Synthetic unmethylated DNA (contains 50% of cytosine) and methylated DNA (contains 50% of 5-methylcytosine) were added into the assay wells at different concentrations and then measured with ab117128.
Product details
Methylated DNA Quantification Kit (Colorimetric) (ab117128) is a complete set of optimized buffers and reagents to quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in a microplate-based format.
It can be paired to use Abcam's Hydroxymethylated DNA Quantification Kit (Colorimetric) (ab117130), which specifically quantifies 5-hmC, for simultaneous quantification of both methylated and hydroxymethylated DNA.
DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quantification of 5-mC content or global methylation status in cells such as tumor cells could provide very useful information for detection and analysis of cancer.
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Publications (38)
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Biologics : targets & therapy 19:251-264 PubMed40321666
2025
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Pharmaceutics 16: PubMed39339187
2024
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Archives of toxicology 98:2143-2152 PubMed38806716
2024
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Frontiers in neuroscience 18:1362497 PubMed38694899
2024
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Developmental cell 59:187-198.e7 PubMed38198888
2024
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Marine drugs 21: PubMed38132942
2023
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Cells 12: PubMed37626872
2023
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Oxidative medicine and cellular longevity 2022:8770136 PubMed36193084
2022
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Journal of translational medicine 20:383 PubMed36038893
2022
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Nature communications 13:4819 PubMed35974013
2022
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com