Methylated DNA Quantification Kit (Colorimetric) (ab117128) is a complete set of optimized buffers and reagents to quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in a microplate-based format.
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- Marine drugs 21:2023Ectoine Globally Hypomethylates DNA in Skin Cells and Suppresses Cancer Proliferation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMajjid A Qaria,Chunyan Xu,Ran Hu,Roua A Alsubki,Mohamed Yassin Ali,Sethupathy Sivasamy,Kotb A Attia,Daochen ZhuPubMed 38132942
- Cells 12:2023Repetitive Transcranial Magnetic Stimulation Reduces Depressive-like Behaviors, Modifies Dendritic Plasticity, and Generates Global Epigenetic Changes in the Frontal Cortex and Hippocampus in a Rodent Model of Chronic Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid Meneses-San Juan,Mónica Lamas,Gerardo Bernabé Ramírez-RodríguezPubMed 37626872
- Oxidative medicine and cellular longevity 2022:87701362022Early Development of Cardiac Fibrosis in Young Old-Father Offspring.Applications:Unspecified applicationReactive species:Unspecified reactive speciesA Ismail,Y Saliba,N FaresPubMed 36193084
- Journal of translational medicine 20:3832022Low mitochondrial DNA copy number induces chemotherapy resistance via epithelial-mesenchymal transition by DNA methylation in esophageal squamous cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuto Kubo,Koji Tanaka,Yasunori Masuike,Tsuyoshi Takahashi,Kotaro Yamashita,Tomoki Makino,Takuro Saito,Kazuyoshi Yamamoto,Tomoyuki Tsujimoto,Takashi Harino,Yukinori Kurokawa,Makoto Yamasaki,Kiyokazu Nakajima,Hidetoshi Eguchi,Yuichiro DokiPubMed 36038893
- Nature communications 13:48192022Primary cilia and SHH signaling impairments in human and mouse models of Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSebastian Schmidt,Malte D Luecken,Dietrich Trümbach,Sina Hembach,Kristina M Niedermeier,Nicole Wenck,Klaus Pflügler,Constantin Stautner,Anika Böttcher,Heiko Lickert,Ciro Ramirez-Suastegui,Ruhel Ahmad,Michael J Ziller,Julia C Fitzgerald,Viktoria Ruf,Wilma D J van de Berg,Allert J Jonker,Thomas Gasser,Beate Winner,Jürgen Winkler,Daniela M Vogt Weisenhorn,Florian Giesert,Fabian J Theis,Wolfgang WurstPubMed 35974013
- Metabolites 12:2022Untargeted Metabolomics Showed Accumulation of One-Carbon Metabolites to Facilitate DNA Methylation during Extracellular Matrix Detachment of Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSuza Mohammad Nur,Mohammed Razeeth Shait Mohammed,Mazin A Zamzami,Hani Choudhry,Aamir Ahmad,Bushra Ateeq,Irfan A Rather,Mohammad Imran KhanPubMed 35323710
- Antioxidants (Basel, Switzerland) 11:2022Antioxidant and Antiaging Properties of a Novel Synergistic Nutraceutical Complex: Readouts from an In Cellulo Study and an In Vivo Prospective, Randomized Trial.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSophia Athanasopoulou,Marianna Kapetanou,Michel Georges Magouritsas,Nikoletta Mougkolia,Polykseni Taouxidou,Michael Papacharalambous,Fotios Sakellaridis,Efstathios GonosPubMed 35326118
- Epigenetics & chromatin 14:482021The methylome of Biomphalaria glabrata and other mollusks: enduring modification of epigenetic landscape and phenotypic traits by a new DNA methylation inhibitor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNelia Luviano,Marie Lopez,Fleur Gawehns,Cristian Chaparro,Paola B Arimondo,Slavica Ivanovic,Patrice David,Koen Verhoeven,Céline Cosseau,Christoph GrunauPubMed 34702322
- Frontiers in cardiovascular medicine 8:6862932021YTHDC2-Mediated circYTHDC2 N6-Methyladenosine Modification Promotes Vascular Smooth Muscle Cells Dysfunction Through Inhibiting Ten-Eleven Translocation 2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Yuan,Yu Liu,Lizhen Zhou,Yan Xue,Zhengde Lu,Jianting GanPubMed 34660707
- Microorganisms 9:2021Epigenetic Response of to Stress: Tracking Methylation Level and Search for Methylation Patterns via Whole-Genome Sequencing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMonika Kubiak-Szymendera,Leszek P Pryszcz,Wojciech Białas,Ewelina CelińskaPubMed 34576693
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Plasma, Tissue, Suspension cells, Serum, Adherent cells
- Assay type
- Quantitative
- Assay time
- 4h
- Sensitivity
- >= 0.2 ng/well
Associated Products
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1 product for Alternative Product
Target data
Alternative names
5 Methycytosine, 5-Me Cytidine, 5-Me Cytosine, 5-Methyl-Cytidine, 5-Methyl-Cytosine, 5-MethylCytidine, 5-MethylCytosine, 5-mC, 5-meC, methyl CpG
What's included?
Recommended products
Methylated DNA Quantification Kit (Colorimetric) (ab117128) is a complete set of optimized buffers and reagents to quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in a microplate-based format.
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Plasma, Tissue, Suspension cells, Serum, Adherent cells
- Assay type
- Quantitative
- Assay time
- 4h
- Assay Platform
- Microplate reader
- Sensitivity
- >= 0.2 ng/well
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Multi
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quantification of 5-mC content or global methylation status in cells such as tumor cells could provide very useful information for detection and analysis of cancer.
Methylated DNA Quantification Kit (Colorimetric) (ab117128) is a complete set of optimized buffers and reagents to quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in a microplate-based format.
It can be paired to use Abcam's Hydroxymethylated DNA Quantification Kit (Colorimetric) (Hydroxymethylated DNA Quantification Kit (Colorimetric) ab117130), which specifically quantifies 5-hmC, for simultaneous quantification of both methylated and hydroxymethylated DNA.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Functional Studies - Methylated DNA Quantification Kit (Colorimetric) (ab117128)
Demonstration of high sensitivity and specificity of methylated DNA detection achieved using ab117128. Synthetic unmethylated DNA (contains 50% of cytosine) and methylated DNA (contains 50% of 5-methylcytosine) were added into the assay wells at different concentrations and then measured with ab117128.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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