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DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC).


Images

Functional Studies - Methylated DNA Quantification Kit (Fluorometric) (AB117129), expandable thumbnail

Publications

Key facts

Detection method

Fluorescent

Sample types

Saliva, Urine, Plasma, Tissue, Suspension cells, Adherent cells

Assay type

Quantitative

Assay time

4h

Sensitivity

>= 50 pg/well

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Alternative names

5 Methycytosine, 5-Me Cytidine, 5-Me Cytosine, 5-Methyl-Cytidine, 5-Methyl-Cytosine, 5-MethylCytidine, 5-MethylCytosine, 5-mC, 5-meC, methyl CpG

What's included?

96 Test
Components
10X Wash Buffer
1 x 28 mL
8-Well Assay Strips (with Frame)
12 x 1 Unit
Binding Solution
1 x 10 mL
Capture Antibody, 1000 µg/mL
1 x 8 µL
Detection Antibody, 400 µg/mL
1 x 16 µL
Enhancer Solution
1 x 16 µL
Fluoro Developer
1 x 16 µL
Fluoro Dilutor
1 x 8 mL
Fluoro Enhancer
1 x 16 µL
Negative Control, 20 µg/mL
1 x 20 µL
Positive Control, 20 µg/mL
1 x 20 µL

Recommended products

DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC).

Key facts

Detection method

Fluorescent

Sample types

Saliva, Urine, Plasma, Tissue, Suspension cells, Adherent cells

Assay type

Quantitative

Assay time

4h

Assay Platform

Microplate reader

Sensitivity

>= 50 pg/well

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quantification of 5-mC content or global methylation status in cells such as tumor cells could provide very useful information for detection and analysis of cancer.

Abcam's Methylated DNA Quantification Kit (Fluorometric) (ab117129) is a complete set of optimized buffers and reagents to quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in a microplate-based format.

It can be paired to use withHydroxymethylated DNA Quantification Kit (Fluorometric) (Hydroxymethylated DNA Quantification Kit (Fluorometric) ab117131), which specifically quantifies 5-hmC, for simultaneously quantification of both methylated and hydroxymethylated DNA.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Functional Studies - Methylated DNA Quantification Kit (Fluorometric) (ab117129), expandable thumbnail

    Functional Studies - Methylated DNA Quantification Kit (Fluorometric) (ab117129)

    Demonstration of high sensitivity and specificity of methylated DNA detection achieved using ab117129. Synthetic unmethylated DNA (contains 50% of cytosine) and methylated DNA (contains 50% of 5-methylcytosine) were added into the assay wells at different concentrations and then measured with ab117129.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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