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AB117129

Methylated DNA Quantification Kit (Fluorometric)

3

(1 Review)

|

(3 Publications)

DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC).

View Alternative Names

5 Methycytosine, 5-Me Cytidine, 5-Me Cytosine, 5-Methyl-Cytidine, 5-Methyl-Cytosine, 5-MethylCytidine, 5-MethylCytosine, 5-mC, 5-meC, methyl CpG

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Functional Studies - Methylated DNA Quantification Kit (Fluorometric) (AB117129)
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Supplier Data

Functional Studies - Methylated DNA Quantification Kit (Fluorometric) (AB117129)

Demonstration of high sensitivity and specificity of methylated DNA detection achieved using ab117129. Synthetic unmethylated DNA (contains 50% of cytosine) and methylated DNA (contains 50% of 5-methylcytosine) were added into the assay wells at different concentrations and then measured with ab117129.

Key facts

Detection method

Fluorescent

Sample types

Saliva, Urine, Plasma, Tissue, Suspension cells, Adherent cells

Assay type

Quantitative

Sensitivity

>= 50 pg/well

Assay time

4h

Assay Platform

Microplate reader

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Product details

DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quantification of 5-mC content or global methylation status in cells such as tumor cells could provide very useful information for detection and analysis of cancer.

Abcam's Methylated DNA Quantification Kit (Fluorometric) (ab117129) is a complete set of optimized buffers and reagents to quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in a microplate-based format.

It can be paired to use withHydroxymethylated DNA Quantification Kit (Fluorometric) (ab117131), which specifically quantifies 5-hmC, for simultaneously quantification of both methylated and hydroxymethylated DNA.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

See full target information 5-Methylcytosine
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular bioengineering 18:29-38 PubMed39949487

2025

Matrix Stiffness-Mediated DNA Methylation in Endothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Paul V Taufalele,Hannah K Kirkham,Cynthia A Reinhart-King

Endocrine connections 6:708-725 PubMed28993426

2017

Effect of diabetes status and hyperglycemia on global DNA methylation and hydroxymethylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jairo Arturo Pinzón-Cortés,Angelina Perna-Chaux,Nicolás Steven Rojas-Villamizar,Angélica Díaz-Basabe,Diana Carolina Polanía-Villanueva,María Fernanda Jácome,Carlos Olimpo Mendivil,Helena Groot,Valeriano López-Segura

Toxicology 390:63-73 PubMed28865727

2017

Altered mitochondrial epigenetics associated with subchronic doxorubicin cardiotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

André Ferreira,Teresa Cunha-Oliveira,Rui F Simões,Filipa S Carvalho,Ana Burgeiro,Kendra Nordgren,Kendall B Wallace,Paulo J Oliveira
View all publications
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Complementary Products

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Functional Studies - Methylated DNA Quantification Kit (Colorimetric) (AB117128)

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AB117131

Hydroxymethylated DNA Quantification Kit (Fluorometric)

Functional Studies - Hydroxymethylated DNA Quantification Kit (Fluorometric) (AB117131)

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Global DNA Methylation Assay Kit (5 Methyl Cytosine, Colorimetric)

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Assay Kits

AB117130

Hydroxymethylated DNA Quantification Kit (Colorimetric)

Functional Studies - Hydroxymethylated DNA Quantification Kit (Colorimetric) (AB117130)

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