Mitochondria Isolation Kit For Tissue and Cultured Cells

• FuncS

Supplier Data

Functional Studies - Mitochondria Isolation Kit For Tissue and Cultured Cells (AB288084)

Mitochondria Integrity Test :

Mitochondria (100 μg) were purified from the mouse liver. Purified mitochondria were analyzed for intactness by using JC-1 dye, which tests the electrochemical proton gradient(ΔΨ) of the inner mitochondrial membrane. Purified mitochondria was thawed on ice and incubated with JC-1 dye (1 μg/ml) for 15 minutes at 37°C. The intact purified mitochondria show aggregation of JC-1 dye whose signal can be measured at Ex/Em = 530/590nm. Treatment with Antimycin A (100 μM) dissipates the mitochondrial membrane potential resulting in reduced florescence signal.

• Schematic Diagram

Supplier Data

Schematic Diagram - Mitochondria Isolation Kit For Tissue and Cultured Cells (AB288084)

Representative image of Mitochondria Isolation Kit For Tissue and Cultured Cells ab288084

Components shown from left to right :

- Permeabilization Reagent

- Storage Buffer II

- Mitochondria Isolation Buffer (2 bottles)

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.