Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit ab115348 is used to determine mitochondrial aldehyde dehydrogenase activity (ALDH2) in a sample.
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- SLAS discovery : advancing life sciences R & D 29:1001542024Aldehyde Dehydrogenase 2 (ALDH2): A novel sorafenib target in hepatocellular carcinoma unraveled by the proteome-wide cellular thermal shift assay.Applications:Unspecified applicationReactive species:Unspecified reactive speciesInês C Ferreira,Estefania Torrejón,Bernardo Abecasis,Bruno M Alexandre,Ricardo A Gomes,Chris Verslype,Jos van Pelt,Ana Barbas,Daniel Simão,Tiago M Bandeiras,Alessio Bortoluzzi,Sofia P RebeloPubMed 38521503
- The EMBO journal 42:e1123042023p53 protects against alcoholic fatty liver disease via ALDH2 inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPengbo Yao,Zhenxi Zhang,Hongchao Liu,Peng Jiang,Wei Li,Wenjing DuPubMed 36825429
- JBMR plus 7:e107232023Gene Therapy to Treat Osteopenia Associated With Chronic Ethanol Consumption and Aldehyde Dehydrogenase 2 Deficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna E Camilleri,Michelle Cung,Fiona M Hart,Odelya E Pagovich,Ronald G Crystal,Matthew B Greenblatt,Katie M StilesPubMed 37065630
- Frontiers in immunology 12:7405622021Pharmacological Activation Of Aldehyde Dehydrogenase 2 Protects Against Heatstroke-Induced Acute Lung Injury by Modulating Oxidative Stress and Endothelial Dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHsiao-Ya Tsai,Yu-Juei Hsu,Cheng-Yo Lu,Min-Chien Tsai,Wan-Chu Hung,Po-Chuan Chen,Jen-Chun Wang,Lung-An Hsu,Yung-Hsin Yeh,Pauling Chu,Shih-Hung TsaiPubMed 34764958
- Oxidative medicine and cellular longevity 2021:16417172021ALDH2/SIRT1 Contributes to Type 1 and Type 2 Diabetes-Induced Retinopathy through Depressing Oxidative Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengshan He,Pan Long,Tao Chen,Kaifeng Li,Dongyu Wei,Yufei Zhang,Wenjun Wang,Yonghe Hu,Yi Ding,Aidong WenPubMed 34725563
- Neural regeneration research 17:185-1932021Neuroprotective effects of Alda-1 mitigate spinal cord injury in mice: involvement of Alda-1-induced ALDH2 activation-mediated suppression of reactive aldehyde mechanisms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMushfiquddin Khan,Fei Qiao,Pavan Kumar,S M Touhidul Islam,Avtar K Singh,Jeseong Won,Inderjit SinghPubMed 34100455
- Nutrients 13:2021Can Cranberry Juice Protect against Rotenone-Induced Toxicity in Rats?Applications:Unspecified applicationReactive species:Unspecified reactive speciesMonika Kurpik,Przemysław Zalewski,Małgorzata Kujawska,Małgorzata Ewertowska,Ewa Ignatowicz,Judyta Cielecka-Piontek,Jadwiga Jodynis-LiebertPubMed 33805023
- Nature metabolism 3:337-3512021Brain ethanol metabolism by astrocytic ALDH2 drives the behavioural effects of ethanol intoxication.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShiyun Jin,Qi Cao,Fanghan Yang,Hongying Zhu,Su Xu,Qi Chen,Ziyi Wang,Yuhong Lin,Resat Cinar,Robert J Pawlosky,Ye Zhang,Wei Xiong,Bin Gao,George F Koob,David M Lovinger,Li ZhangPubMed 33758417
- Brain research 1758:1473352021GSNOR and ALDH2 alleviate traumatic spinal cord injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMushfiquddin Khan,Fei Qiao,S M Touhidul Islam,Tajinder S Dhammu,Pavan Kumar,Jeseong Won,Avtar K Singh,Inderjit SinghPubMed 33545099
- International journal of molecular sciences 21:2020Aldehyde Dehydrogenase 2 Ameliorates Chronic Alcohol Consumption-Induced Atrial Fibrillation through Detoxification of 4-HNE.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLung-An Hsu et. al.PubMed 32932651
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Extracts
- Assay type
- Enzyme activity
- Reactive species
- Mouse, Rat, Cow, Dog, Human
- Range
- 60 - 250 µg/mL
- Assay time
- 4h
Target data
Function
Catalyzes the oxidation of medium and long chain aliphatic aldehydes to fatty acids. Active on a variety of saturated and unsaturated aliphatic aldehydes between 6 and 24 carbons in length (PubMed:18035827, PubMed:18182499, PubMed:22633490, PubMed:25047030, PubMed:9133646, PubMed:9662422). Responsible for conversion of the sphingosine 1-phosphate (S1P) degradation product hexadecenal to hexadecenoic acid (PubMed:22633490).
Alternative names
ALDH10, FALDH, ALDH3A2, Aldehyde dehydrogenase family 3 member A2, Aldehyde dehydrogenase 10, Fatty aldehyde dehydrogenase, Microsomal aldehyde dehydrogenase
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Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit ab115348 is used to determine mitochondrial aldehyde dehydrogenase activity (ALDH2) in a sample.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Extracts
- Assay type
- Enzyme activity
- Reactive species
- Mouse, Rat, Cow, Dog, Human
- Range
- 60 - 250 µg/mL
- Assay time
- 4h
- Assay Platform
- Microplate reader
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Sample 1 | n 20 | C.V. 5 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Sample 1 | n 4 | C.V. 12 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit ab115348 is used to determine mitochondrial aldehyde dehydrogenase activity (ALDH2) in a sample. The enzyme is captured within the wells of the microplate and activity is determined by following the production of NADH in the following ALDH2 catalyzed reaction:
acetaldehyde + NAD+ -> acid + NADH
The generation of NADH in the aldehyde dehydrogenase assay protocol is coupled to the 1:1 reduction of a reporter dye to yield a colored (yellow) reaction product whose concentration can be monitored by measuring the increase in absorbance at 450 nm (Dye molar extinction coefficient - 37000 M-1cm-1). ab115348 immunocaptures in each well only native ALDH2 from the chosen sample; this removes all other enzymes, including unrelated aldehyde dehydrogenases.
Reagent dye, Coupler and NAD+ are shipped lyophilized. Before use rehydrate by adding 0.25 mL pure water to each tube and vortex each tube thoroughly to dissolve. After hydration unused amounts of these three materials should be stored at -80°C. Store all other components store at 4°C.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Aldehyde Dehydrogenase (ALDH) an important enzyme responsible for oxidizing aldehydes into carboxylic acids participates in detoxifying aldehydes produced during various metabolic processes. ALDH has several isoforms including the well-studied ALDH2 which possesses a molecular mass of approximately 56 kDa. ALDH2 is found in many tissues but highest levels are in liver and kidney playing a significant role in processing endogenous aldehydes and those derived from external sources such as alcohol metabolism.
- Biological function summary
Aldehyde Dehydrogenase influences the metabolism of aldehydrates aiding in the protection of cells from aldehyde-induced stress. ALDH exists within a larger family of enzymes and does not typically operate as part of a large complex. It helps maintain the balance of reactive aldehyde species in the cell through oxidation facilitating cellular protection against oxidative damage.
- Pathways
Aldehyde Dehydrogenase plays an integral part in alcohol metabolism and the larger detoxification pathways. The enzyme ALDH2 is notably involved in the ethanol degradation pathway where it metabolizes acetaldehyde to acetate a less toxic compound. It interacts closely with alcohol dehydrogenase which initially forms acetaldehyde from ethanol establishing a sequential relationship within this critical metabolic pathway.
- Associated diseases and disorders
ALDH2's role is closely linked with alcoholism and Asian flush syndrome. ALDH2 deficiency impairs acetaldehyde detoxification leading to accumulation resulting in facial flushing and increased risk of alcoholism-related complications. Interaction with other proteins such as cytochrome P450 enzymes can impact the severity of these conditions as they are involved in overlapping detoxification pathways that handle a variety of substrates and drugs.
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Functional Studies - Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit (ab115348)
Determination of effect reactive species on ALDH2 activity.
The mitochondrial location of ALDH2 may increase the exposure of this enzyme to high levels of reactive oxygen and nitrogen species generated in mitochondria. ALDH2 has been shown to be specifically
inactivated by nitrative stress, particularly by modification or crosslinking of an active site cysteine. To demonstrate this rat liver homogenate was exposed in vitro to a dilution series of peroxynitirite at concentrations - 0, 25, 50, 100, 200, 400, 800, 1600 µM. After exposure the samples were extracted, diluted to 0.25 mg/mL and loaded into microplate wells in duplicate. After following the assay protocol as described above, the activity of each sample was measured and graphed against peroxynitrite exposure concentration.
The ALDH2 activity after exposure was reduced with an IC50approximately 210 µM.
Functional Studies - Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit (ab115348)
Reference Sample graph
Functional Studies - Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit (ab115348)
Reference sample graph
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