AB115348

Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit

Name: Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit | Abcam
Brand: Abcam Limited
SKU: AB115348
Price: 635 USD
Availability: InStock

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(36 Publications)

Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit ab115348 is used to determine mitochondrial aldehyde dehydrogenase activity (ALDH2) in a sample.

View Alternative Names

ALDH10, FALDH, ALDH3A2, Aldehyde dehydrogenase family 3 member A2, Aldehyde dehydrogenase 10, Fatty aldehyde dehydrogenase, Microsomal aldehyde dehydrogenase

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Functional Studies - Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit (AB115348)

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Functional Studies - Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit (AB115348)

Determination of effect reactive species on ALDH2 activity.
The mitochondrial location of ALDH2 may increase the exposure of this enzyme to high levels of reactive oxygen and nitrogen species generated in mitochondria. ALDH2 has been shown to be specifically inactivated by nitrative stress, particularly by modification or crosslinking of an active site cysteine. To demonstrate this rat liver homogenate was exposed in vitro to a dilution series of peroxynitirite at concentrations - 0, 25, 50, 100, 200, 400, 800, 1600 µM. After exposure the samples were extracted, diluted to 0.25 mg/mL and loaded into microplate wells in duplicate. After following the assay protocol as described above, the activity of each sample was measured and graphed against peroxynitrite exposure concentration. The ALDH2 activity after exposure was reduced with an IC₅₀approximately 210 µM.

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Functional Studies - Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit (AB115348)

Reference sample graph

FuncS

Supplier Data

Functional Studies - Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit (AB115348)

Reference Sample graph

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Mouse, Rat, Cow, Dog, Human

Assay type

Enzyme activity

Range

60 - 250 µg/mL

Assay time

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit ab115348 is used to determine mitochondrial aldehyde dehydrogenase activity (ALDH2) in a sample. The enzyme is captured within the wells of the microplate and activity is determined by following the production of NADH in the following ALDH2 catalyzed reaction:

acetaldehyde + NAD⁺ -> acid + NADH

The generation of NADH in the aldehyde dehydrogenase assay protocol is coupled to the 1:1 reduction of a reporter dye to yield a colored (yellow) reaction product whose concentration can be monitored by measuring the increase in absorbance at 450 nm (Dye molar extinction coefficient - 37000 M^-1cm^-1). ab115348 immunocaptures in each well only native ALDH2 from the chosen sample; this removes all other enzymes, including unrelated aldehyde dehydrogenases.

Reagent dye, Coupler and NAD+ are shipped lyophilized. Before use rehydrate by adding 0.25 mL pure water to each tube and vortex each tube thoroughly to dissolve. After hydration unused amounts of these three materials should be stored at -80°C. Store all other components store at 4°C.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "12" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 20, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab115348-1x96Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"10X Blocking Buffer", "number":"AB115348-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"20X Buffer", "number":"AB115348-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"Extraction Buffer (ab260490)", "number":"AB115348-CMP03", "productcode":"" }, { "size":"1 x 0.25 mL", "name":"100X Reagent Dye", "number":"AB115348-CMP08", "productcode":"" }, { "size":"1 x 24 mL", "name":"1X Base Buffer", "number":"AB115348-CMP01", "productcode":"" }, { "size":"1 x 0.25 mL", "name":"100X NAD+", "number":"AB115348-CMP07", "productcode":"" }, { "size":"1 x 0.25 mL", "name":"100X acetaldehyde (2.5 M)", "number":"AB115348-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"96 well microplate (12 strips)", "number":"AB115348-CMP06", "productcode":"" }, { "size":"1 x 0.25 mL", "name":"100X Coupler", "number":"AB115348-CMP05", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aldehyde Dehydrogenase (ALDH) an important enzyme responsible for oxidizing aldehydes into carboxylic acids participates in detoxifying aldehydes produced during various metabolic processes. ALDH has several isoforms including the well-studied ALDH2 which possesses a molecular mass of approximately 56 kDa. ALDH2 is found in many tissues but highest levels are in liver and kidney playing a significant role in processing endogenous aldehydes and those derived from external sources such as alcohol metabolism.

Biological function summary

Aldehyde Dehydrogenase influences the metabolism of aldehydrates aiding in the protection of cells from aldehyde-induced stress. ALDH exists within a larger family of enzymes and does not typically operate as part of a large complex. It helps maintain the balance of reactive aldehyde species in the cell through oxidation facilitating cellular protection against oxidative damage.

Pathways

Aldehyde Dehydrogenase plays an integral part in alcohol metabolism and the larger detoxification pathways. The enzyme ALDH2 is notably involved in the ethanol degradation pathway where it metabolizes acetaldehyde to acetate a less toxic compound. It interacts closely with alcohol dehydrogenase which initially forms acetaldehyde from ethanol establishing a sequential relationship within this critical metabolic pathway.

ALDH2's role is closely linked with alcoholism and Asian flush syndrome. ALDH2 deficiency impairs acetaldehyde detoxification leading to accumulation resulting in facial flushing and increased risk of alcoholism-related complications. Interaction with other proteins such as cytochrome P450 enzymes can impact the severity of these conditions as they are involved in overlapping detoxification pathways that handle a variety of substrates and drugs.

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Product protocols

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Target data

Catalyzes the oxidation of medium and long chain aliphatic aldehydes to fatty acids. Active on a variety of saturated and unsaturated aliphatic aldehydes between 6 and 24 carbons in length (PubMed : 18035827, PubMed : 18182499, PubMed : 22633490, PubMed : 25047030, PubMed : 9133646, PubMed : 9662422). Responsible for conversion of the sphingosine 1-phosphate (S1P) degradation product hexadecenal to hexadecenoic acid (PubMed : 22633490).

See full target information ALDH3A2

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Publications (36)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 78:103437 PubMed39591904

2024

Antrodia cinnamomea triterpenoids attenuate cardiac hypertrophy via the SNW1/RXR/ALDH2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yinghua Ma,Yunxia Wang,Gulinigaer Anwaier,Nuerbiye Tuerdi,Yangchang Wu,Yinyue Huang,Boyang Qin,Haoyue Ma,Qiao Zhang,Dalei Wu,Kewu Zeng,Rong Qi

Cardiovascular diabetology 23:380 PubMed39462342

2024

ALDH2 mediates the effects of sodium-glucose cotransporter 2 inhibitors (SGLT2i) on improving cardiac remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Han Liu,Bingchen Jiang,Rui Hua,Xuehao Liu,Bao Qiao,Xiangxin Zhang,Xilong Liu,Wenjun Wang,Qiuhuan Yuan,Bailu Wang,Shujian Wei,Yuguo Chen

JCI insight 9: PubMed39226171

2024

Aldehyde dehydrogenase 2 preserves kidney function by countering acrolein-induced metabolic and mitochondrial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Szu-Yuan Li,Ming-Tsun Tsai,Yu-Ming Kuo,Hui-Min Yang,Zhen-Jie Tong,Hsiao-Wei Cheng,Chih-Ching Lin,Hsiang-Tsui Wang

Journal of diabetes investigation 15:684-692 PubMed38713732

2024

Mitochondrial ALDH2 improves ß-cell survival and function against doxorubicin-induced apoptosis by targeting CK2 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Udayakumar Karunakaran,Eun Yeong Ha,Suma Elumalai,Kyu Chang Won,Jun Sung Moon

International journal of stem cells 17:284-297 PubMed38604748

2024

Impaired Osteogenesis in Human Induced Pluripotent Stem Cells with Acetaldehyde Dehydrogenase 2 Mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Jooyoung Lim,Heeju Han,Se In Jung,Yeri Alice Rim,Ji Hyeon Ju

SLAS discovery : advancing life sciences R & D 29:100154 PubMed38521503

2024

Aldehyde Dehydrogenase 2 (ALDH2): A novel sorafenib target in hepatocellular carcinoma unraveled by the proteome-wide cellular thermal shift assay.

Applications

Unspecified application

Species

Unspecified reactive species

Inês C Ferreira,Estefania Torrejón,Bernardo Abecasis,Bruno M Alexandre,Ricardo A Gomes,Chris Verslype,Jos van Pelt,Ana Barbas,Daniel Simão,Tiago M Bandeiras,Alessio Bortoluzzi,Sofia P Rebelo

Nature communications 15:2594 PubMed38519490

2024

The aldehyde dehydrogenase 2 rs671 variant enhances amyloid β pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Wang,Jiayu Wang,Yashuang Chen,Xiaojing Qian,Shiqi Luo,Xue Wang,Chao Ma,Wei Ge

Redox biology 69:102994 PubMed38128451

2023

Mitochondrial aldehyde dehydrogenase-2 coordinates the hydrogen sulfide - AMPK axis to attenuate high glucose-induced pancreatic β-cell dysfunction by glutathione antioxidant system.

Applications

Unspecified application

Species

Unspecified reactive species

Udayakumar Karunakaran,Suma Elumalai,Seung Min Chung,Kathrin Maedler,Kyu Chang Won,Jun Sung Moon

Life sciences 331:122038 PubMed37619835

2023

Estrogen-mediated mitigation of cardiac oxidative stress in ovariectomized rats is associated with upregulated cardiac circadian clock Per2 and heart-specific miRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Syed Anees Ahmed,Baohong Zhang,Abdel A Abdel-Rahman

The EMBO journal 42:e112304 PubMed36825429

2023

p53 protects against alcoholic fatty liver disease via ALDH2 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Pengbo Yao,Zhenxi Zhang,Hongchao Liu,Peng Jiang,Wei Li,Wenjing Du

View all publications

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Product promise

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Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit

Functional Studies - Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit (AB115348)

Functional Studies - Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit (AB115348)

Functional Studies - Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit (AB115348)

Key facts

Detection method

Sample types

Reacts with

Assay type

Range

Assay time

Assay Platform

Complementary Products

Sample Prep & Detection Kits

Proteins & Peptides

Secondary Antibodies

Assay Kits

Primary Antibodies

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (36)

Product promise