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Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit ab115348 is used to determine mitochondrial aldehyde dehydrogenase activity (ALDH2) in a sample.

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Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Assay type

Enzyme activity

Reactive species

Mouse, Rat, Cow, Dog, Human

Range

60 - 250 µg/mL

Assay time

4h

Images

Target data

What's included?

1 x 96 Tests
Components
100X Coupler
1 x 0.25 mL
100X NAD+
1 x 0.25 mL
100X Reagent Dye
1 x 0.25 mL
100X acetaldehyde (2.5 M)
1 x 0.25 mL
10X Blocking Buffer
1 x 6 mL
1X Base Buffer
1 x 24 mL
20X Buffer
1 x 20 mL
96 well microplate (12 strips)
1 x 1 Unit
Extraction Buffer (ab260490)
1 x 15 mL

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Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit ab115348 is used to determine mitochondrial aldehyde dehydrogenase activity (ALDH2) in a sample.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Assay type

Enzyme activity

Reactive species

Mouse, Rat, Cow, Dog, Human

Range

60 - 250 µg/mL

Assay time

4h

Assay Platform

Microplate reader

Precision

Intra assay

Sample
Sample 1
n
20
mean
-
SD
-
C.V.
5

Inter assay

Sample
Sample 1
n
4
mean
-
SD
-
C.V.
12

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit ab115348 is used to determine mitochondrial aldehyde dehydrogenase activity (ALDH2) in a sample. The enzyme is captured within the wells of the microplate and activity is determined by following the production of NADH in the following ALDH2 catalyzed reaction:

acetaldehyde + NAD+ -> acid + NADH

The generation of NADH in the aldehyde dehydrogenase assay protocol is coupled to the 1:1 reduction of a reporter dye to yield a colored (yellow) reaction product whose concentration can be monitored by measuring the increase in absorbance at 450 nm (Dye molar extinction coefficient - 37000 M-1cm-1). ab115348 immunocaptures in each well only native ALDH2 from the chosen sample; this removes all other enzymes, including unrelated aldehyde dehydrogenases.

Reagent dye, Coupler and NAD+ are shipped lyophilized. Before use rehydrate by adding 0.25 mL pure water to each tube and vortex each tube thoroughly to dissolve. After hydration unused amounts of these three materials should be stored at -80°C. Store all other components store at 4°C.

Product promise

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Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

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