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AB287847

Mitochondrial Complex I Activity Colorimetric Assay Kit

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(4 Publications)

The Mitochondrial Complex I assay kit ab287847 is a fast and reliable method to determine the activity of complex I in isolated mitochondria.
3 Images
Enzyme activity assay - Mitochondrial Complex I Activity Colorimetric Assay Kit (AB287847)
  • Enzyme activity assay

Supplier Data

Enzyme activity assay - Mitochondrial Complex I Activity Colorimetric Assay Kit (AB287847)

Mitochondrial Complex 1 Activity Colorimetric Assay Kit Standard Curve.

Standard curve for oxidized Complex I dye ab287847

Enzyme activity assay - Mitochondrial Complex I Activity Colorimetric Assay Kit (AB287847)
  • Enzyme activity assay

Supplier Data

Enzyme activity assay - Mitochondrial Complex I Activity Colorimetric Assay Kit (AB287847)

Mitochondrial Complex 1 Activity Colorimetric Assay Kit Activity Assay.

OD600 with varying concentrations of Bovine heart mitochondria obtained commercially

Enzyme activity assay - Mitochondrial Complex I Activity Colorimetric Assay Kit (AB287847)
  • Enzyme activity assay

Supplier Data

Enzyme activity assay - Mitochondrial Complex I Activity Colorimetric Assay Kit (AB287847)

Mitochondrial Complex 1 Activity Colorimetric Assay Kit Activity Assay 2.

Complex I Activity in isolated Bovine heart mitochondria (obtained commercially) and jurkat cell mitochondria with and without Complex I inhibitor rotenone.

Key facts

Detection method

Colorimetric

Sample types

Purified mitochondria

Assay type

Enzyme activity

Sensitivity

= 0.1 mU/well

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Mitochondrial Complex I assay kit ab287847 is a fast and reliable method to determine the activity of complex I in isolated mitochondria. It is useful for respiration studies in isolated mitochondria and may be used to study effects of toxicants, drugs and other environmental conditions on mitochondrial complex I activity. This kit uses decylubiquinone, an analog of ubiquinone, as an electron acceptor that gets converted to decylubiquinol through the catalytic activity of Complex I. The Complex I dye that absorbs light at 600 nm in its oxidized form is used as a terminal electron acceptor that accepts electrons from decylubiquinol. Complex I activity is determined colorimetrically by recording the change in absorbance of reduced Complex I dye at 600 nm. Specific Complex I activity is obtained by subtracting the activity in presence of Complex I inhibitor rotenone from total activity. This kit can detect as low as 0.1 mU / well and is linear up to 7 mU / well.

This product is manufactured by BioVision, an Abcam company and was previously called K968 Mitochondrial Complex I Activity Colorimetric Assay Kit. K968-100 is the same size as the 96 test size of ab287847.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab287847-96Test", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"96-Well Half Area Plate", "number":"AB287847-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"100X NADH Stock", "number":"AB287847-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 47", "number":"AB287847-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Complex I Dye", "number":"AB287847-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Decylubiquinone", "number":"AB287847-CMP01", "productcode":"" }, { "size":"1 x 100 µL", "name":"Complex I Inhibitor", "number":"AB287847-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39201759

2024

DGAT1 and DGAT2 Inhibitors for Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) Management: Benefits for Their Single or Combined Application.

Applications

Unspecified application

Species

Unspecified reactive species

Miriam Longo,Erika Paolini,Pietro Di Benedetto,Elena Tomassini,Marica Meroni,Paola Dongiovanni

Nature communications 15:6640 PubMed39103324

2024

Gasdermin-E-mediated pyroptosis drives immune checkpoint inhibitor-associated myocarditis via cGAS-STING activation.

Applications

Unspecified application

Species

Unspecified reactive species

Si-Jia Sun,Xiao-Dong Jiao,Zhi-Gang Chen,Qi Cao,Jia-Hui Zhu,Qi-Rui Shen,Yi Liu,Zhen Zhang,Fang-Fang Xu,Yu Shi,Jie Tong,Shen-Xi Ouyang,Jiang-Tao Fu,Yi Zhao,Jun Ren,Dong-Jie Li,Fu-Ming Shen,Pei Wang

Antioxidants (Basel, Switzerland) 12: PubMed36830013

2023

Mitochondrial-Targeted Antioxidant MitoQ-Mediated Autophagy: A Novel Strategy for Precise Radiation Protection.

Applications

Unspecified application

Species

Unspecified reactive species

Xingting Bao,Xiongxiong Liu,Qingfeng Wu,Fei Ye,Zheng Shi,Dan Xu,Jinhua Zhang,Zhihui Dou,Guomin Huang,Hong Zhang,Chao Sun

The FEBS journal 290:1596-1624 PubMed36239430

2022

Early loss of endogenous NAD following rotenone treatment leads to mitochondrial dysfunction and Sarm1 induction that is ameliorated by PARP inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Ankita Sarkar,Sourav Dutta,Malinki Sur,Semanti Chakraborty,Puja Dey,Piyali Mukherjee
View all publications
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Product promise

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