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AB287844

Mitochondrial Complex III Activity Assay Kit

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(13 Publications)

Mitochondrial Complex III Activity Assay kit (ab287844) is based on the reduction of cytochrome c by Complex III. The absorbance of reduced cytochrome c is 550 nm using a colorimetric plate reader.<br><br>Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Functional Studies - Mitochondrial Complex III Activity Assay Kit (AB287844)
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Supplier Data

Functional Studies - Mitochondrial Complex III Activity Assay Kit (AB287844)

a) Standard curve for Cytochrime C when reduced with DTT.

b) Complex III activity in increasing amounts of bovine heart mitochondria

c) Activity of Complex III in isolated bovine heart mitochondria in absence and presence of Antimycin A (units/μg protein).

Key facts

Detection method

Colorimetric

Sample types

Purified mitochondria

Results type

Quantitative

Sensitivity

= 10 mU/mL

Assay Platform

Microplate

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Product details

<p>Mitochondrial Complex III Activity Assay kit (ab287844) is based on the reduction of cytochrome c through the activity of Complex III. The absorbance of reduced cytochrome c can be measured at 550 nm. It is a fast and reliable method to determine the activity of complex III in isolated mitochondria. The kit can detect as low as 10 mU/ml and is linear up to 30 mU/ml.</p>

<p><b>Other Notes</b><br>
This product is manufactured by BioVision, an Abcam company and was previously called K520 Mitochondrial Complex III Activity Assay Kit. K520-100 is the same size as the 100 test size of ab287844.</p>

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab287844-100Test", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"96-Well Half Area Plate", "number":"AB287844-CMP02", "productcode":"" }, { "size":"4 x 1 Vial", "name":"Oxidized Cytochrome c", "number":"AB287844-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"Complex III Assay Buffer", "number":"AB287844-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"Antimycin A", "number":"AB287844-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"DTT IV", "number":"AB287844-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

ACS omega 10:24018-24029 PubMed40547630

2025

Klotho Suppresses Indoxyl Sulfate-Mediated Apoptosis in Human Kidney Proximal Tubular (HK-2) Cells through Modulating the AKT/Nrf2 Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Chien-Yao Sun,Yao-Tsung Lin,Yu-Ting Huang,Hui-Ching Cheng,Wan-Ching Chou,Yu-Tzu Chang,Tsai-Chieh Ling,Pei-Ling Hsieh,Kun-Ling Tsai

The Journal of clinical investigation 135: PubMed39786963

2025

Loss of Cpt1a results in elevated glucose-fueled mitochondrial oxidative phosphorylation and defective hematopoietic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jue Li,Jie Bai,Vincent T Pham,Michihiro Hashimoto,Maiko Sezaki,Qili Shi,Qiushi Jin,Chenhui He,Amy Armstrong,Tian Li,Mingzhe Pan,Shujun Liu,Yu Luan,Hui Zeng,Paul R Andreassen,Gang Huang

The EMBO journal 43:5941-5971 PubMed39420094

2024

An Orai1 gain-of-function tubular aggregate myopathy mouse model phenocopies key features of the human disease.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Zhao,Antonio Michelucci,Laura Pietrangelo,Sundeep Malik,Linda Groom,Jennifer Leigh,Thomas N O'Connor,Takahiro Takano,Paul D Kingsley,James Palis,Simona Boncompagni,Feliciano Protasi,Robert T Dirksen

International journal of molecular sciences 25: PubMed39201759

2024

DGAT1 and DGAT2 Inhibitors for Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) Management: Benefits for Their Single or Combined Application.

Applications

Unspecified application

Species

Unspecified reactive species

Miriam Longo,Erika Paolini,Pietro Di Benedetto,Elena Tomassini,Marica Meroni,Paola Dongiovanni

Clinical and experimental pharmacology & physiology 51:e13860 PubMed38584327

2024

NPAS2, transcriptionally activated by ARRB1, promotes the malignant behaviours of lung adenocarcinoma cells and regulates the reprogramming of glucose metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Shenglan Wang,Chunhong Huang,Yanbin Zheng,Xinjie Wu,Yutong Zhong

Pflugers Archiv : European journal of physiology 476:939-948 PubMed38446167

2024

Locally applied heat stress during exercise training may promote adaptations to mitochondrial enzyme activities in skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Ed Maunder,Andrew King,Jeffrey A Rothschild,Matthew J Brick,Warren B Leigh,Christopher P Hedges,Troy L Merry,Andrew E Kilding

International journal of biological sciences 19:4327-4339 PubMed37705748

2023

Sirtuin-3 activates the mitochondrial unfolded protein response and reduces cerebral ischemia/reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xie Xiaowei,Xu Qian,Zhou Dingzhou

Antioxidants (Basel, Switzerland) 12: PubMed36830013

2023

Mitochondrial-Targeted Antioxidant MitoQ-Mediated Autophagy: A Novel Strategy for Precise Radiation Protection.

Applications

Unspecified application

Species

Unspecified reactive species

Xingting Bao,Xiongxiong Liu,Qingfeng Wu,Fei Ye,Zheng Shi,Dan Xu,Jinhua Zhang,Zhihui Dou,Guomin Huang,Hong Zhang,Chao Sun

Nature communications 13:7100 PubMed36402842

2022

Phosphorylation disrupts long-distance electron transport in cytochrome c.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandre M J Gomila,Gonzalo Pérez-Mejías,Alba Nin-Hill,Alejandra Guerra-Castellano,Laura Casas-Ferrer,Sthefany Ortiz-Tescari,Antonio Díaz-Quintana,Josep Samitier,Carme Rovira,Miguel A De la Rosa,Irene Díaz-Moreno,Pau Gorostiza,Marina I Giannotti,Anna Lagunas

The FEBS journal 290:1596-1624 PubMed36239430

2022

Early loss of endogenous NAD following rotenone treatment leads to mitochondrial dysfunction and Sarm1 induction that is ameliorated by PARP inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Ankita Sarkar,Sourav Dutta,Malinki Sur,Semanti Chakraborty,Puja Dey,Piyali Mukherjee
View all publications
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Product promise

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