This Mitochondrial Complex III Activity Assay kit (ab287844) is based on this reduction of cytochrome c through the activity of Complex III. The absorbance of reduced cytochrome c can be measured at 550 nm. It is a fast and reliable method to determine the activity of complex III in isolated mitochondria. The kit can detect as low as 10 mU/ml and is linear up to 30 mU/ml.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- International journal of biological sciences 19:4327-43392023Sirtuin-3 activates the mitochondrial unfolded protein response and reduces cerebral ischemia/reperfusion injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXie Xiaowei,Xu Qian,Zhou DingzhouPubMed 37705748
- Antioxidants (Basel, Switzerland) 12:2023Mitochondrial-Targeted Antioxidant MitoQ-Mediated Autophagy: A Novel Strategy for Precise Radiation Protection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXingting Bao,Xiongxiong Liu,Qingfeng Wu,Fei Ye,Zheng Shi,Dan Xu,Jinhua Zhang,Zhihui Dou,Guomin Huang,Hong Zhang,Chao SunPubMed 36830013
- Nature communications 13:71002022Phosphorylation disrupts long-distance electron transport in cytochrome c.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexandre M J Gomila,Gonzalo Pérez-Mejías,Alba Nin-Hill,Alejandra Guerra-Castellano,Laura Casas-Ferrer,Sthefany Ortiz-Tescari,Antonio Díaz-Quintana,Josep Samitier,Carme Rovira,Miguel A De la Rosa,Irene Díaz-Moreno,Pau Gorostiza,Marina I Giannotti,Anna LagunasPubMed 36402842
- The FEBS journal 290:1596-16242022Early loss of endogenous NAD following rotenone treatment leads to mitochondrial dysfunction and Sarm1 induction that is ameliorated by PARP inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnkita Sarkar,Sourav Dutta,Malinki Sur,Semanti Chakraborty,Puja Dey,Piyali MukherjeePubMed 36239430
- Frontiers in pharmacology 13:8933362022Exploring Ganweikang Tablet as a Candidate Drug for NAFLD Through Network Pharmacology Analysis and Experimental Validation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuanrui Ma,Xinyu Wang,Jing Zhang,Yun Zhao,Yunqing Hua,Chao Zhang,Guobin Zheng,Guangyan Yang,Jianli Guan,Huahuan Li,Meng Li,Lin Kang,Jiaqing Xiang,Guanwei Fan,Shu YangPubMed 35774609
- The Journal of biological chemistry 298:1020502022PACT establishes a posttranscriptional brake on mitochondrial biogenesis by promoting the maturation of miR-181c.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAsli E Dogan,Syed M Hamid,Asli D Yildirim,Zehra Yildirim,Ganes Sen,Celine E Riera,Roberta A Gottlieb,Ebru ErbayPubMed 35598827
- Journal of advanced research 41:39-482022DNA-PKcs promotes sepsis-induced multiple organ failure by triggering mitochondrial dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRongjun Zou,Jun Tao,Junxiong Qiu,Huimin Lu,Jianhua Wu,Hang Zhu,Ruibing Li,David Mui,Sam Toan,Xing Chang,Hao Zhou,Xiaoping FanPubMed 36328752
Key facts
- Detection method
- Colorimetric
- Sample types
- Purified mitochondria
- Assay type
- Quantitative
- Sensitivity
- = 10 mU/mL
What's included?
Recommended products
This Mitochondrial Complex III Activity Assay kit (ab287844) is based on this reduction of cytochrome c through the activity of Complex III. The absorbance of reduced cytochrome c can be measured at 550 nm. It is a fast and reliable method to determine the activity of complex III in isolated mitochondria. The kit can detect as low as 10 mU/ml and is linear up to 30 mU/ml.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
- Colorimetric
- Sample types
- Purified mitochondria
- Assay type
- Quantitative
- Assay Platform
- Microplate
- Sensitivity
- = 10 mU/mL
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
This Mitochondrial Complex III Activity Assay kit (ab287844) is based on this reduction of cytochrome c through the activity of Complex III. The absorbance of reduced cytochrome c can be measured at 550 nm. It is a fast and reliable method to determine the activity of complex III in isolated mitochondria. The kit can detect as low as 10 mU/ml and is linear up to 30 mU/ml.
This product is manufactured by BioVision, an Abcam company and was previously called K520 Mitochondrial Complex III Activity Assay Kit. K520-100 is the same size as the 100 test size of ab287844.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Product promise
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1 product image
Functional Studies - Mitochondrial Complex III Activity Assay Kit (ab287844)
a) Standard curve for Cytochrime C when reduced with DTT.
b) Complex III activity in increasing amounts of bovine heart mitochondria
c) Activity of Complex III in isolated bovine heart mitochondria in absence and presence of Antimycin A (units/μg protein).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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