JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB109907

MitoTox™ Complex V OXPHOS Activity Assay Kit

Be the first to review this product! Submit a review

|

(28 Publications)

MitoTox™ Complex V OXPHOS Activity Assay Kit (ab109907) is designed for testing the direct inhibitory effect of compounds on Complex V activity in only 4 hours.
2 Images
Functional Studies - MitoTox™ Complex V OXPHOS Activity Assay Kit (AB109907)
  • FuncS

Supplier Data

Functional Studies - MitoTox™ Complex V OXPHOS Activity Assay Kit (AB109907)

Typical dose response curve for oligomycin. Assay was performed following the Dose Response Assay Procedure using oligomycin, a well known Complex V inhibitor. Oligomycin was prepared in DMSO to generate a 10 mM stock. Starting with a 50 μM final concentration in well, 1 : 10 serial dilutions of oligomycin were generated.

Functional Studies - MitoTox™ Complex V OXPHOS Activity Assay Kit (AB109907)
  • FuncS

Supplier Data

Functional Studies - MitoTox™ Complex V OXPHOS Activity Assay Kit (AB109907)

Dose response curve for aurovertin, a non-competitive inhibitor of Complex V.

Key facts

Detection method

Colorimetric

Sample types

Inhibitor compounds

Reacts with

Rat, Cow, Human

Assay type

Direct

Assay time

4h

Assay Platform

Microplate reader

style
left-column

Complementary Products

Assay Kits

AB109903

MitoTox™ Complex I OXPHOS Activity Assay Kit

Functional Studies - MitoTox™ Complex I OXPHOS Activity Assay Kit (AB109903)

  • 0
  • 0
Assay Kits

AB109908

Complex II Activity Assay Kit (Colorimetric)

Functional Studies - Complex II Activity Assay Kit (Colorimetric) (AB109908)

  • 5
  • 2
Assay Kits

AB109909

Complex IV Human Enzyme Activity Microplate Assay Kit

Functional Studies - Complex IV Human Enzyme Activity Microplate Assay Kit (AB109909)

  • 5
  • 2
Assay Kits

AB109905

MitoTox™ Complex II + III OXPHOS Activity Assay Kit

Functional Studies - MitoTox™ Complex II + III OXPHOS Activity Assay Kit (AB109905)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

MitoTox™ Complex V OXPHOS Activity Assay Kit (ab109907) is designed for testing the direct inhibitory effect of compounds on Complex V activity in only 4 hours. Complex V extracted from the provided bovine heart mitochondria (a rich source of Complex V) is immunocaptured by specific antibodies on the plate. Complex V activity can be observed as decrease in absorbance at OD 340 nm. The intra-assay and inter-assay variation of this assay are both < 10%.

Inhibitory effects of compounds on Complex I activity can be tested in two different ways: 1. Screening format, where up to 23 compounds can be tested at a single concentration in triplicate; 2. Dose response (IC50) format, where two compounds known to affect Complex V activity can be tested at 11 different data points in triplicate.

Testing for mitochondrial function has become a key aspect of drug discovery. Mitochondria can be affected by drug treatment, resulting into cardio- and hepatotoxic side effects that can lead to drug withdrawal from the market. Therefore, there is increasing emphasis on testing the impact on mitochondria early on in the drug development process to reduce failure rates during preclinical and clinical phases.

Store Phospholipids, Bovine heart mitochondria and Activity Buffer at -80°C. Store all other components at 4°C.

Related products

Review the mitochondrial assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.

style
left-column

What's included?

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab109907-96Test", "assetComponentDetails": [ { "size":"1 x 360 µL", "name":"Bovine heart mitochondria", "number":"AB109907-CMP07", "productcode":"" }, { "size":"2 x 1 Unit", "name":"12-channel reagent reservoirs", "number":"AB109907-CMP03", "productcode":"" }, { "size":"1 x 5 mL", "name":"1X Mito Buffer", "number":"AB109907-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-coated 96-well microplate", "number":"AB109907-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Phospholipids", "number":"AB109907-CMP08", "productcode":"" }, { "size":"1 x 5 mL", "name":"20X Wash Buffer", "number":"AB109907-CMP05", "productcode":"" }, { "size":"1 x 100 µL", "name":"Detergent", "number":"AB109907-CMP02", "productcode":"" }, { "size":"1 x 24 mL", "name":"Complex V Activity Buffer", "number":"AB109907-CMP01", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Complex V commonly called ATP synthase plays an important role in cellular energy production. This enzyme complex exists in the inner mitochondrial membrane and is approximately 600 kDa in size. Complex V facilitates the conversion of ADP and inorganic phosphate into ATP during oxidative phosphorylation. It consists of multiple subunits including Fo and F1 components where Fo functions as a proton channel while F1 performs ATP synthesis. Complex V is ubiquitously expressed across various tissues reflecting its essential role in energy generation.
Biological function summary

Complex V is important for maintaining cellular energy homeostasis by generating ATP the cell's energy currency. It operates as part of the mitochondrial respiratory chain complex essential for efficient energy transfer and storage. This complex plays a significant role in cellular metabolism by linking the electrochemical gradient formed by protons across the inner mitochondrial membrane to the mechanical rotation of its subunits leading to ATP formation. The ATP synthase products are imperative for processes demanding high energy such as muscle contraction and biosynthetic pathways.

Pathways

Complex V integrates into major metabolic routes primarily oxidative phosphorylation and the broader mitochondrial electron transport chain. It works in conjunction with complexes I to IV facilitating energy conversion via a proton gradient and ATP generation. Complex V's role complements proteins such as cytochrome c and ubiquinone which shuttle electrons within the electron transport chain ensuring a seamless energy production process essential for aerobic respiration.

Complex V dysfunction can cause mitochondrial diseases and disorders related to energy metabolism including Leigh syndrome and mitochondrial myopathy. These conditions often result from mutations in the ATP synthase subunits leading to impaired ATP production and altered cellular functions. Additional related proteins in these diseases include NADH dehydrogenase and succinate dehydrogenase which when dysfunctional further contribute to defects in the electron transport chain compounding the energy production challenges in affected individuals.
style
left-column

Product protocols

style
left-column

Target data

style
left-column

Publications (28)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 11:15 PubMed39828731

2025

CRAT downregulation promotes ovarian cancer progression by facilitating mitochondrial metabolism through decreasing the acetylation of PGC-1α.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Zhang,Shuhua Zhao,Xiaohui Lv,Yan Gao,Qian Guo,Yanjie Ren,Yuanyuan He,Yihua Jin,Hong Yang,Shujuan Liu,Xiaohong Zhang

Clinical and experimental pharmacology & physiology 51:e13860 PubMed38584327

2024

NPAS2, transcriptionally activated by ARRB1, promotes the malignant behaviours of lung adenocarcinoma cells and regulates the reprogramming of glucose metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Shenglan Wang,Chunhong Huang,Yanbin Zheng,Xinjie Wu,Yutong Zhong

Cell reports 43:114067 PubMed38583150

2024

Bacterial muropeptides promote OXPHOS and suppress mitochondrial stress in mammals.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Tian,Mingxue Cui,Min Han

Cell communication and signaling : CCS 21:192 PubMed37537600

2023

Decreased MFN2 activates the cGAS-STING pathway in diabetic myocardial ischaemia-reperfusion by triggering the release of mitochondrial DNA.

Applications

Unspecified application

Species

Unspecified reactive species

Yonghong Xiong,Yan Leng,Hao Tian,Xinqi Deng,Wenyuan Li,Wei Li,Zhongyuan Xia

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2301071 PubMed37401167

2023

Adamantaniline Derivatives Target ATP5B to Inhibit Translation of Hypoxia Inducible Factor-1α.

Applications

Unspecified application

Species

Unspecified reactive species

Huiti Li,Yali Liu,Zian Xue,Li Zhang,Xiaoxue Ruan,Jintong Yang,Zhongjiao Fan,Hongfang Zhao,Yu Cao,Guoqiang Chen,Ying Xu,Lu Zhou

Function (Oxford, England) 4:zqac065 PubMed36654930

2023

Integration of High-Throughput Imaging and Multiparametric Metabolic Profiling Reveals a Mitochondrial Mechanism of Tenofovir Toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Adam Pearson,Dominik Haenni,Jamal Bouitbir,Matthew Hunt,Brendan A I Payne,Ashwin Sachdeva,Rachel K Y Hung,Frank A Post,John Connolly,Stellor Nlandu-Khodo,Nevena Jankovic,Milica Bugarski,Andrew M Hall

PLoS genetics 19:e1010565 PubMed36656833

2023

Altered energy metabolism in Fatal Familial Insomnia cerebral organoids is associated with astrogliosis and neuronal dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Simote T Foliaki,Anna Smith,Benjamin Schwarz,Eric Bohrnsen,Catharine M Bosio,Katie Williams,Christina D Orrú,Hailey Lachenauer,Bradley R Groveman,Cathryn L Haigh

Research (Washington, D.C.) 2022:0001 PubMed39285950

2022

PGAM5-Mediated PHB2 Dephosphorylation Contributes to Diabetic Cardiomyopathy by Disrupting Mitochondrial Quality Surveillance.

Applications

Unspecified application

Species

Unspecified reactive species

Rongjun Zou,Jun Tao,Jie He,Chaojie Wang,Songtao Tan,Yu Xia,Xing Chang,Ruibing Li,Ge Wang,Hao Zhou,Xiaoping Fan

Frontiers in endocrinology 13:895593 PubMed35957832

2022

plays an important role in the pathogenesis of metabolic-associated fatty liver disease by regulating hepatic lipid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Yongqiang Ma,Guangshun Chen,Junfang Yi,Qiang Li,Zhi Tan,Wenling Fan,Xiaohua Luo,Zhiyong He,Zhongzhou Si,Jiequn Li

Communications biology 4:775 PubMed34163008

2021

Cyanocobalamin prevents cardiomyopathy in type 1 diabetes by modulating oxidative stress and DNMT-SOCS1/3-IGF-1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Masao Kakoki,Purushotham V Ramanathan,John R Hagaman,Ruriko Grant,Jennifer C Wilder,Joan M Taylor,J Charles Jennette,Oliver Smithies,Nobuyo Maeda-Smithies
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com