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AB118972

MMP-3 Activity Assay Kit (Fluorometric)

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(17 Publications)

In Abcam's MMP-3 Activity Assay Kit, MMP-3 hydrolyzes a specific FRET substrate to release the quenched fluorescent group Mca, which can be detected fluorometrically at Ex/Em=325/393 nm.

View Alternative Names

STMY1, MMP3, Stromelysin-1, SL-1, Matrix metalloproteinase-3, Transin-1, MMP-3

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Functional Studies - MMP-3 Activity Assay Kit (Fluorometric) (AB118972)
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Supplier Data

Functional Studies - MMP-3 Activity Assay Kit (Fluorometric) (AB118972)

MMP-3 Positive Control

Functional Studies - MMP-3 Activity Assay Kit (Fluorometric) (AB118972)
  • FuncS

Supplier Data

Functional Studies - MMP-3 Activity Assay Kit (Fluorometric) (AB118972)

MMP-3 Mca Standard

Key facts

Detection method

Fluorescent

Sample types

Urine, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk

Assay type

Enzyme activity

Sensitivity

< 0.05 mU/well

Assay time

1h

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

In Abcam's MMP-3 Activity Assay Kit, MMP-3 hydrolyzes a specific FRET substrate to release the quenched fluorescent group Mca, which can be detected fluorometrically at Ex/Em=325/393 nm. The kit provides a rapid, simple, sensitive and reliable test which can also be used as a high throughput assay of MMP-3 activity. The assay sensitivity is < 50 μU.

Visit our FAQs page for tips and troubleshooting.

This product is manufactured by BioVision, an Abcam company and was previously called K783 MMP-3 Activity Fluorometric Assay Kit. K783-100 is the same size as the 100 test size of ab118972.

The matrix metalloproteinase-3 (MMP-3, stromelysin-1) exhibits a number of activities that would make it a particularly good tumor promoter. Like several other MMPs, MMP-3 was first cloned and later recloned as a cancer-specific gene. In addition to degrading numerous extracellular matrix components, MMP-3 can activate gelatinase B, the collagenases and several serpin-type serine proteinase inhibitors. Moreover, it can release a number of cell surface molecules, including E-cadherin, a known contributor to cancer development.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab118972-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"MMP-3 Positive Control", "number":"AB118972-CMP02", "productcode":"" }, { "size":"1 x 20 µL", "name":"MCA Peptide Standard", "number":"AB118972-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"MMP-1/3 Assay Buffer", "number":"AB118972-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"MMP-3 Substrate", "number":"AB118972-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MMP3 also known as stromelysin-1 is a member of the matrix metalloproteinase (MMP) family. These proteins are zinc-dependent endopeptidases. MMP3 specifically has a molecular weight of approximately 54 kDa and is responsible for degrading extracellular matrix components such as collagen fibronectin and laminin. MMP3 is expressed in various tissues including fibroblasts chondrocytes and endothelial cells. It plays a significant role in tissue remodeling wound healing and potentially aids in the breakdown of cellular matrices.
Biological function summary

MMP3 contributes to multiple physiological and pathological processes. It supports the remodeling of connective tissues and assists in normal developmental processes. MMP3 can work as part of a complex with other MMP proteins which can enhance or inhibit its activity. The balance of MMPs including MMP3 regulates the extracellular matrix turnover and is important for maintaining tissue homeostasis.

Pathways

MMP3 participates in pathways involved in tissue remodeling and inflammatory responses. It is part of cellular pathways regulated by cytokines like interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-alpha). These pathways link MMP3 to other proteins such as MMP1 and MMP9 which perform overlapping and distinct roles in matrix degradation during inflammation and tissue repair.

MMP3 is implicated in conditions like arthritis and cardiovascular diseases. MMP3 contributes to cartilage degradation in rheumatoid arthritis and is associated with the breakdown of vascular structures in atherosclerosis. Proteins like MMP9 and MMP13 often interact with MMP3 in these pathological conditions forming a network that exacerbates tissue damage and disease progression.
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Product protocols

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Target data

Metalloproteinase with a rather broad substrate specificity that can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates different molecules including growth factors, plasminogen or other matrix metalloproteinases such as MMP9 (PubMed : 11029580, PubMed : 1371271). Once released into the extracellular matrix (ECM), the inactive pro-enzyme is activated by the plasmin cascade signaling pathway (PubMed : 2383557). Acts also intracellularly (PubMed : 22265821). For example, in dopaminergic neurons, gets activated by the serine protease HTRA2 upon stress and plays a pivotal role in DA neuronal degeneration by mediating microglial activation and alpha-synuclein/SNCA cleavage (PubMed : 21330369). In addition, plays a role in immune response and possesses antiviral activity against various viruses such as vesicular stomatitis virus, influenza A virus (H1N1) and human herpes virus 1 (PubMed : 35940311). Mechanistically, translocates from the cytoplasm into the cell nucleus upon virus infection to influence NF-kappa-B activities (PubMed : 35940311).
See full target information MMP3
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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Bioengineering & translational medicine 10:e10741 PubMed40385549

2025

Establishment of a chemoresistant laryngeal cancer cell model to study chemoresistance and chemosensitization responses via transcriptomic analysis and a tumor-on-a-chip platform.

Applications

Unspecified application

Species

Unspecified reactive species

Christian R Moya-Garcia,Meghana Munipalle,Alain Pacis,Nader Sadeghi,Maryam Tabrizian,Nicole Y K Li-Jessen

International journal of molecular sciences 26: PubMed40362252

2025

Upregulation of MMP3 Promotes Cisplatin Resistance in Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mariela Rivera-Serrano,Marienid Flores-Colón,Fatima Valiyeva,Loyda M Meléndez,Pablo E Vivas-Mejía

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 44:1102-1116 PubMed38388375

2024

Hemorrhagic stroke-induced subtype of inflammatory reactive astrocytes disrupts blood-brain barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Liu,Yiyan Guo,Shiyu Deng,Shiyi Zhou,Shengju Wu,Tingting Chen,Xiaojing Shi,Muyassar Mamtilahun,Tongtong Xu,Ze Liu,Hanlai Li,Zhijun Zhang,Hengli Tian,Won-Suk Chung,Jixian Wang,Guo-Yuan Yang,Yaohui Tang

Molecules and cells 46:627-636 PubMed37641936

2023

Avenanthramide-C Shows Potential to Alleviate Gingival Inflammation and Alveolar Bone Loss in Experimental Periodontitis.

Applications

Unspecified application

Species

Unspecified reactive species

Su-Jin Kim,Se Hui Lee,Binh Do Quang,Thanh-Tam Tran,Young-Gwon Kim,Jun Ko,Weon-Young Choi,Sun Young Lee,Je-Hwang Ryu

Science advances 9:eadf6537 PubMed37075118

2023

Matrix metalloproteinase-3 (MMP-3)-mediated gene therapy for glaucoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jeffrey O'Callaghan,Conor Delaney,Merissa O'Connor,Joseph van Batenburg-Sherwood,Martin Schicht,Elke Lütjen-Drecoll,Natalie Hudson,Sorcha Ni Dhubhghaill,Peter Humphries,Chris Stanley,Annahita Keravala,Thomas Chalberg,Matthew S Lawrence,Matthew Campbell

International journal of molecular sciences 24: PubMed37108220

2023

Diplacone Isolated from Mature Fruit Induces Ferroptosis-Mediated Cell Death through Mitochondrial Ca Influx and Mitochondrial Permeability Transition.

Applications

Unspecified application

Species

Unspecified reactive species

Myung-Ji Kang,Hyung Won Ryu,Eun Sol Oh,Yu Na Song,Yang Hoon Huh,Ji-Yoon Park,Seon Min Oh,Su-Yeon Lee,Yhun Jung Park,Doo-Young Kim,Hyunju Ro,Sung-Tae Hong,Su Ui Lee,Dong-Oh Moon,Mun-Ock Kim

Nature 605:747-753 PubMed35585241

2022

PHGDH heterogeneity potentiates cancer cell dissemination and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Matteo Rossi,Patricia Altea-Manzano,Margherita Demicco,Ginevra Doglioni,Laura Bornes,Marina Fukano,Anke Vandekeere,Alejandro M Cuadros,Juan Fernández-García,Carla Riera-Domingo,Cristina Jauset,Mélanie Planque,H Furkan Alkan,David Nittner,Dongmei Zuo,Lindsay A Broadfield,Sweta Parik,Antonino Alejandro Pane,Francesca Rizzollo,Gianmarco Rinaldi,Tao Zhang,Shao Thing Teoh,Arin B Aurora,Panagiotis Karras,Ines Vermeire,Dorien Broekaert,Joke Van Elsen,Maximilian M L Knott,Martin F Orth,Sofie Demeyer,Guy Eelen,Lacey E Dobrolecki,Ayse Bassez,Thomas Van Brussel,Karl Sotlar,Michael T Lewis,Harald Bartsch,Manfred Wuhrer,Peter van Veelen,Peter Carmeliet,Jan Cools,Sean J Morrison,Jean-Christophe Marine,Diether Lambrechts,Massimiliano Mazzone,Gregory J Hannon,Sophia Y Lunt,Thomas G P Grünewald,Morag Park,Jacco van Rheenen,Sarah-Maria Fendt

Nature cardiovascular research 1:322-333 PubMed36246779

2022

Smad3 regulates smooth muscle cell fate and mediates adverse remodeling and calcification of the atherosclerotic plaque.

Applications

Unspecified application

Species

Unspecified reactive species

Paul Cheng,Robert C Wirka,Juyong Brian Kim,Hyun-Jung Kim,Trieu Nguyen,Ramendra Kundu,Quanyi Zhao,Disha Sharma,Albert Pedroza,Manabu Nagao,Dharini Iyer,Michael P Fischbein,Thomas Quertermous

The Journal of pharmacy and pharmacology 72:843-851 PubMed32196690

2020

Kruppel-like factor 2 disturb non-small cell lung cancer energy metabolism by inhibited glutamine consumption.

Applications

Unspecified application

Species

Unspecified reactive species

Song Xiao,Yan Jin-Xiang,Tian Long,Lu Xiu-Rong,Gao Hong,Yan Jie-Cheng,Zhang Fei

Scientific reports 10:1386 PubMed31992771

2020

Estradiol/GPER affects the integrity of mammary duct-like structures in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Deng,Yoshio Miki,Akira Nakanishi
View all publications
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