In Abcam's MMP-3 Activity Assay Kit, MMP-3 hydrolyzes a specific FRET substrate to release the quenched fluorescent group Mca, which can be detected fluorometrically at Ex/Em=325/393 nm.
Images
Publications
Filter by
- Molecules and cells 46:627-6362023Avenanthramide-C Shows Potential to Alleviate Gingival Inflammation and Alveolar Bone Loss in Experimental Periodontitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSu-Jin Kim,Se Hui Lee,Binh Do Quang,Thanh-Tam Tran,Young-Gwon Kim,Jun Ko,Weon-Young Choi,Sun Young Lee,Je-Hwang RyuPubMed 37641936
- Science advances 9:eadf65372023Matrix metalloproteinase-3 (MMP-3)-mediated gene therapy for glaucoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeffrey O'Callaghan,Conor Delaney,Merissa O'Connor,Joseph van Batenburg-Sherwood,Martin Schicht,Elke Lütjen-Drecoll,Natalie Hudson,Sorcha Ni Dhubhghaill,Peter Humphries,Chris Stanley,Annahita Keravala,Thomas Chalberg,Matthew S Lawrence,Matthew CampbellPubMed 37075118
- International journal of molecular sciences 24:2023Diplacone Isolated from Mature Fruit Induces Ferroptosis-Mediated Cell Death through Mitochondrial Ca Influx and Mitochondrial Permeability Transition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMyung-Ji Kang,Hyung Won Ryu,Eun Sol Oh,Yu Na Song,Yang Hoon Huh,Ji-Yoon Park,Seon Min Oh,Su-Yeon Lee,Yhun Jung Park,Doo-Young Kim,Hyunju Ro,Sung-Tae Hong,Su Ui Lee,Dong-Oh Moon,Mun-Ock KimPubMed 37108220
- Nature 605:747-7532022PHGDH heterogeneity potentiates cancer cell dissemination and metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatteo Rossi,Patricia Altea-Manzano,Margherita Demicco,Ginevra Doglioni,Laura Bornes,Marina Fukano,Anke Vandekeere,Alejandro M Cuadros,Juan Fernández-García,Carla Riera-Domingo,Cristina Jauset,Mélanie Planque,H Furkan Alkan,David Nittner,Dongmei Zuo,Lindsay A Broadfield,Sweta Parik,Antonino Alejandro Pane,Francesca Rizzollo,Gianmarco Rinaldi,Tao Zhang,Shao Thing Teoh,Arin B Aurora,Panagiotis Karras,Ines Vermeire,Dorien Broekaert,Joke Van Elsen,Maximilian M L Knott,Martin F Orth,Sofie Demeyer,Guy Eelen,Lacey E Dobrolecki,Ayse Bassez,Thomas Van Brussel,Karl Sotlar,Michael T Lewis,Harald Bartsch,Manfred Wuhrer,Peter van Veelen,Peter Carmeliet,Jan Cools,Sean J Morrison,Jean-Christophe Marine,Diether Lambrechts,Massimiliano Mazzone,Gregory J Hannon,Sophia Y Lunt,Thomas G P Grünewald,Morag Park,Jacco van Rheenen,Sarah-Maria FendtPubMed 35585241
- Nature cardiovascular research 1:322-3332022Smad3 regulates smooth muscle cell fate and mediates adverse remodeling and calcification of the atherosclerotic plaque.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPaul Cheng,Robert C Wirka,Juyong Brian Kim,Hyun-Jung Kim,Trieu Nguyen,Ramendra Kundu,Quanyi Zhao,Disha Sharma,Albert Pedroza,Manabu Nagao,Dharini Iyer,Michael P Fischbein,Thomas QuertermousPubMed 36246779
- The Journal of pharmacy and pharmacology 72:843-8512020Kruppel-like factor 2 disturb non-small cell lung cancer energy metabolism by inhibited glutamine consumption.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSong Xiao,Yan Jin-Xiang,Tian Long,Lu Xiu-Rong,Gao Hong,Yan Jie-Cheng,Zhang FeiPubMed 32196690
- Scientific reports 10:13862020Estradiol/GPER affects the integrity of mammary duct-like structures in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Deng et. al.PubMed 31992771
- Phytomedicine : international journal of phytother 62:1529642019Phloretin suppresses metastasis by targeting protease and inhibits cancer stemness and angiogenesis in human cervical cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi-Hsuan Hsiao et. al.PubMed 31153059
- Nature communications 9:50222018Macrophage-derived netrin-1 promotes abdominal aortic aneurysm formation by activating MMP3 in vascular smooth muscle cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTarik Hadi,Ludovic Boytard,Michele Silvestro,Dornazsadat Alebrahim,Samson Jacob,Jordyn Feinstein,Krista Barone,Wes Spiro,Susan Hutchison,Russell Simon,Debra Rateri,Florence Pinet,David Fenyo,Mark Adelman,Kathryn J Moore,Holger K Eltzschig,Alan Daugherty,Bhama RamkhelawonPubMed 30479344
- Nucleic acids research 46:1912-19262018Interplay between RNASEH2 and MOV10 controls LINE-1 retrotransposition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJongsu Choi,Sung-Yeon Hwang,Kwangseog AhnPubMed 29315404
Key facts
- Detection method
- Fluorescent
- Sample types
- Urine, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk
- Assay type
- Enzyme activity
- Reactive species
- Mammals
- Assay time
- 1h
- Sensitivity
- < 0.05 mU/well
Target data
Function
Metalloproteinase with a rather broad substrate specificity that can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates different molecules including growth factors, plasminogen or other matrix metalloproteinases such as MMP9 (PubMed:11029580, PubMed:1371271). Once released into the extracellular matrix (ECM), the inactive pro-enzyme is activated by the plasmin cascade signaling pathway (PubMed:2383557). Acts also intracellularly (PubMed:22265821). For example, in dopaminergic neurons, gets activated by the serine protease HTRA2 upon stress and plays a pivotal role in DA neuronal degeneration by mediating microglial activation and alpha-synuclein/SNCA cleavage (PubMed:21330369). In addition, plays a role in immune response and possesses antiviral activity against various viruses such as vesicular stomatitis virus, influenza A virus (H1N1) and human herpes virus 1 (PubMed:35940311). Mechanistically, translocates from the cytoplasm into the cell nucleus upon virus infection to influence NF-kappa-B activities (PubMed:35940311).
Alternative names
STMY1, MMP3, Stromelysin-1, SL-1, Matrix metalloproteinase-3, Transin-1, MMP-3
What's included?
Recommended products
In Abcam's MMP-3 Activity Assay Kit, MMP-3 hydrolyzes a specific FRET substrate to release the quenched fluorescent group Mca, which can be detected fluorometrically at Ex/Em=325/393 nm.
Key facts
- Detection method
- Fluorescent
- Sample types
- Urine, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk
- Assay type
- Enzyme activity
- Reactive species
- Mammals
- Assay time
- 1h
- Assay Platform
- Microplate reader
- Sensitivity
- < 0.05 mU/well
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
In Abcam's MMP-3 Activity Assay Kit, MMP-3 hydrolyzes a specific FRET substrate to release the quenched fluorescent group Mca, which can be detected fluorometrically at Ex/Em=325/393 nm. The kit provides a rapid, simple, sensitive and reliable test which can also be used as a high throughput assay of MMP-3 activity. The assay sensitivity is < 50 μU.
Visit our FAQs page for tips and troubleshooting.
This product is manufactured by BioVision, an Abcam company and was previously called K783 MMP-3 Activity Fluorometric Assay Kit. K783-100 is the same size as the 100 test size of ab118972.
The matrix metalloproteinase-3 (MMP-3, stromelysin-1) exhibits a number of activities that would make it a particularly good tumor promoter. Like several other MMPs, MMP-3 was first cloned and later recloned as a cancer-specific gene. In addition to degrading numerous extracellular matrix components, MMP-3 can activate gelatinase B, the collagenases and several serpin-type serine proteinase inhibitors. Moreover, it can release a number of cell surface molecules, including E-cadherin, a known contributor to cancer development.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MMP3 also known as stromelysin-1 is a member of the matrix metalloproteinase (MMP) family. These proteins are zinc-dependent endopeptidases. MMP3 specifically has a molecular weight of approximately 54 kDa and is responsible for degrading extracellular matrix components such as collagen fibronectin and laminin. MMP3 is expressed in various tissues including fibroblasts chondrocytes and endothelial cells. It plays a significant role in tissue remodeling wound healing and potentially aids in the breakdown of cellular matrices.
- Biological function summary
MMP3 contributes to multiple physiological and pathological processes. It supports the remodeling of connective tissues and assists in normal developmental processes. MMP3 can work as part of a complex with other MMP proteins which can enhance or inhibit its activity. The balance of MMPs including MMP3 regulates the extracellular matrix turnover and is important for maintaining tissue homeostasis.
- Pathways
MMP3 participates in pathways involved in tissue remodeling and inflammatory responses. It is part of cellular pathways regulated by cytokines like interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-alpha). These pathways link MMP3 to other proteins such as MMP1 and MMP9 which perform overlapping and distinct roles in matrix degradation during inflammation and tissue repair.
- Associated diseases and disorders
MMP3 is implicated in conditions like arthritis and cardiovascular diseases. MMP3 contributes to cartilage degradation in rheumatoid arthritis and is associated with the breakdown of vascular structures in atherosclerosis. Proteins like MMP9 and MMP13 often interact with MMP3 in these pathological conditions forming a network that exacerbates tissue damage and disease progression.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Functional Studies - MMP-3 Activity Assay Kit (Fluorometric) (ab118972)
MMP-3 Positive Control
Functional Studies - MMP-3 Activity Assay Kit (Fluorometric) (ab118972)
MMP-3 Mca Standard
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com