MMP Activity Assay Kit (Fluorometric Red) ab112147 uses a fluorescence resonance energy transfer (FRET) peptide as a MMP substrate.
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- Scientific reports 13:101482023Enteral administration of the protease inhibitor gabexate mesilate preserves vascular function in experimental trauma/hemorrhagic shock.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNathalia J D Moreira,Fernando Dos Santos,Joyce B Li,Federico Aletti,Maria Claudia C Irigoyen,Erik B KistlerPubMed 37349360
- Metabolites 12:2022Cryoprotective Metabolites Are Sourced from Both External Diet and Internal Macromolecular Reserves during Metabolic Reprogramming for Freeze Tolerance in Drosophilid Fly, .Applications:Unspecified applicationReactive species:Unspecified reactive speciesMartin Moos,Jaroslava Korbelová,Tomáš Štětina,Stanislav Opekar,Petr Šimek,Robert Grgac,Vladimír KoštálPubMed 35208237
- Translational psychiatry 11:5542021A human iPSC-astroglia neurodevelopmental model reveals divergent transcriptomic patterns in schizophrenia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAttila Szabo,Ibrahim A Akkouh,Matthieu Vandenberghe,Jordi Requena Osete,Timothy Hughes,Vivi Heine,Olav B Smeland,Joel C Glover,Ole A Andreassen,Srdjan DjurovicPubMed 34716291
- Scientific reports 11:66552021Identification of extracellular matrix proteins secreted by human dermal fibroblasts cultured in 3D electrospun scaffolds.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAtena Malakpour-Permlid,Irina Buzzi,Cecilia Hegardt,Fredrik Johansson,Stina OredssonPubMed 33758206
- Circulation research 126:25-372019Rapamycin-Loaded Biomimetic Nanoparticles Reverse Vascular Inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristian Boada,Assaf Zinger,Christopher Tsao,Picheng Zhao,Jonathan O Martinez,Kelly Hartman,Tomoyuki Naoi,Roman Sukhoveshin,Manuela Sushnitha,Roberto Molinaro,Barry Trachtenberg,John P Cooke,Ennio TasciottiPubMed 31647755
- Scientific reports 9:144362019Normal mammary gland development after MMTV-Cre mediated conditional PAK4 gene depletion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesParisa Rabieifar et. al.PubMed 31594963
- Acta biomaterialia 100:292-3052019Hyaluronic acid as a macromolecular crowding agent for production of cell-derived matrices.Applications:Unspecified applicationReactive species:Unspecified reactive speciesD Shendi et. al.PubMed 31568877
- Cancer cell 32:639-653.e62017Organelle Specific O-Glycosylation Drives MMP14 Activation, Tumor Growth, and Metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnh Tuan Nguyen,Joanne Chia,Manon Ros,Kam Man Hui,Frederic Saltel,Frederic BardPubMed 29136507
- Drug delivery 24:942-9512017Assessment of solid microneedle rollers to enhance transmembrane delivery of doxycycline and inhibition of MMP activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbbie Omolu,Maryse Bailly,Richard M DayPubMed 28618841
- International journal of nanomedicine 12:3433-34462017Induction of mitophagy-mediated antitumor activity with folate-appended methyl-β-cyclodextrin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKazuhisa Kameyama et. al.PubMed 28496320
Key facts
- Detection method
- Fluorescent
- Sample types
- Purified protein, Tissue Lysate
- Assay type
- Direct
- Reactive species
- Mammals
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MMP Activity Assay Kit (Fluorometric Red) ab112147 uses a fluorescence resonance energy transfer (FRET) peptide as a MMP substrate.
Key facts
- Detection method
- Fluorescent
- Sample types
- Purified protein, Tissue Lysate
- Assay type
- Direct
- Reactive species
- Mammals
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
MMP Activity Assay Kit (Fluorometric Red) ab112147 uses a fluorescence resonance energy transfer (FRET) peptide as a MMP substrate. In the intact FRET peptide, the fluorescence of one part is quenched by the other. Upon cleavage into two separate fragments by MMPs, the fluorescence is recovered.
The MMP assy is designed to check the general activity of a MMP enzyme in a tissue sample. It can also be used to screen MMP inhibitors when a purified MMP enzyme is used.
With excellent fluorescence quantum yield and longer wavelength, the substrate used in this assay shows less interference from autofluorescence of test compounds and cellular components and is much more sensitive than an EDANS/Dabcyl FRET substrate.
The MMP assay signal can be easily read by a fluorescence microplate reader at Ex/Em = 540/590 nm. The pH-independent fluorescence makes the assay reading available for the whole physiological pH range.
The high photostability of this FRET peptide provides a useful imaging probe. Many labs have used this kit for the high throughput screening of MMP inhibitors as potential anticancer drug candidates. This assay might be also used for monitoring cancer cells.
ab112147 should be stored Desiccated
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Matrix Metalloproteinases (MMPs) are a group of enzymes that break down extracellular matrix components. These are sometimes called matrixins. MMPs have a mass typically ranging from 19 to 120 kDa depending on the specific MMP enzyme. They are expressed in various tissues throughout the body including the skin lung liver and in the context of cancer cells. MMP activity is important for normal physiological processes like tissue remodeling and wound healing. Researchers frequently use MMP activity assay kits to measure the activity levels of these enzymes in biological samples for both research and clinical diagnostics.
- Biological function summary
MMPs play significant roles in tissue remodeling and cell signaling. MMPs are not usually found as part of a complex but individual enzymes can be regulated through inhibitor proteins such as TIMPs (tissue inhibitors of metalloproteinases) which bind to MMPs and modulate their enzymatic activity. This regulation is essential to maintain tissue homeostasis and any imbalance may result in pathological conditions. MMPs influence processes such as morphogenesis angiogenesis and metastasis highlighting their importance in developmental biology and cancer research.
- Pathways
MMPs are integral to processes involving the breakdown of the extracellular matrix. Notably MMPs are central components of the degradative pathways facilitating cell migration during immune responses and metastasis. MMPs interact closely with protein inhibitors like TIMPs and these interactions play key roles in the balance of proteolytic activity. Additionally MMPs relate to other proteins like integrins and cytokines linking them to pathways such as the TGF-beta signaling pathway and the Wnt signaling pathway.
- Associated diseases and disorders
MMPs have been implicated in conditions such as cancer and arthritis. In cancer abnormal MMP activity can elevate tumor invasion and metastasis where MMPs collaborate with proteins like E-cadherin and VEGF modulating the tumor microenvironment. In arthritis MMPs contribute to the degradation of cartilage intensifying inflammatory responses and tissue damage. Understanding MMP function and its inhibition offers therapeutic potential for managing these diseases.
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Functional Studies - MMP Activity Assay Kit (Fluorometric - Red) (ab112147)
Tissues were lysed with RIPA buffer and activated with 2 mM APMA (1:1) for 1,2 and 3 hours at 37°C. Samples were then diluted to 5 mg/mL and 10 mg/mL with Assay Buffer. 50 μl of MMP containing sample was mixed with MMP Red Substrate. The fluorescence signal was monitored 30 min after the start of the reaction by using a microplate reader with a filter set of Ex/Em = 540/595 nm. The reading from all wells was subtracted with the reading from substrate control, which contains MMP Red Substrate but no MMPs.
Functional Studies - MMP Activity Assay Kit (Fluorometric - Red) (ab112147)
Tissues were lysed with RIPA buffer and activated with 2 mM APMA (1:1) for 1,2 and 3 hours at 37°C. Samples were then diluted to 5 mg/mL and 10 mg/mL with Assay Buffer. 50 μl of MMP containing sample was mixed with MMP Red Substrate. The fluorescence signal was monitored 30 min after the start of the reaction by using a microplate reader with a filter set of Ex/Em = 540/595 nm. The reading from all wells was subtracted with the reading from substrate control, which contains MMP Red Substrate but no MMPs.
Functional Studies - MMP Activity Assay Kit (Fluorometric - Red) (ab112147)
MMPs were activated with 2 mM APMA (1:1). Samples were then diluted to 0.6 μg/mL (30 ng per well) with Assay Buffer. 50 μl of MMP containing sample was mixed with MMP Red Substrate. The fluorescence signal was monitored 30 min after the start of the reaction by using a microplate reader with a filter set of Ex/Em = 540/595 nm. The reading from all wells was subtracted with the reading from substrate control, which contains MMP Red Substrate but no MMPs.
Functional Studies - MMP Activity Assay Kit (Fluorometric - Red) (ab112147)
MMPs were activated with 2 mM APMA (1:1). Samples were then diluted to 0.6 μg/mL (30 ng per well) with Assay Buffer. 50 μl of MMP containing sample was mixed with MMP Red Substrate. The fluorescence signal was monitored 30 min after the start of the reaction by using a microplate reader with a filter set of Ex/Em = 540/595 nm. The reading from all wells was subtracted with the reading from substrate control, which contains MMP Red Substrate but no MMPs.
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