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AB139457

MMP10 Inhibitor Screening Assay Kit (Fluorometric)

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Abcam MMP10 Inhibitor Screening Assay Kit (Fluorometric) (ab139457) is a complete assay system designed to screen MMP10 inhibitors using a quenched fluorogenic peptide: MMP Fluorogenic Substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 [Mca=(7-methoxycoumarin-4-yl)-acetyl; Dpa=N-3-(2,4-dinitrophenyl)-L-α-β-diaminopropionyl].
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Functional Studies - MMP10 Inhibitor Screening Assay Kit (Fluorometric) (AB139457)
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Supplier Data

Functional Studies - MMP10 Inhibitor Screening Assay Kit (Fluorometric) (AB139457)

Inhibition of MMP10 by NNGH

Key facts

Detection method

Fluorescent

Sample types

Inhibitor compounds

Assay type

Enzyme activity

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam MMP10 Inhibitor Screening Assay Kit (Fluorometric) (ab139457) is a complete assay system designed to screen MMP10 inhibitors using a quenched fluorogenic peptide: MMP Fluorogenic Substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 [Mca=(7-methoxycoumarin-4-yl)-acetyl; Dpa=N-3-(2,4-dinitrophenyl)-L-α-β-diaminopropionyl]. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties. The assays are performed in a convenient 96-well microplate format.

This kit is useful to screen inhibitors of MMP10, a potential therapeutic target. The MMP inhibitor NNGH is also included as a prototypic control inhibitor.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab139457-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"96-well White Microplate 1/2 Volume", "number":"AB139457-CMP06", "productcode":"" }, { "size":"1 x 30 µL", "name":"MMP10 Enzyme (Human, Recombinant)", "number":"AB139457-CMP01", "productcode":"" }, { "size":"1 x 50 µL", "name":"MMP Inhibitor", "number":"AB139457-CMP04", "productcode":"" }, { "size":"1 x 50 µL", "name":"MMP Calibration Standard", "number":"AB139457-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"MMP Fluorogenic Substrate", "number":"AB139457-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"Fluorometric Assay Buffer", "number":"AB139457-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MMP10 also known as stromelysin-2 is a member of the matrix metalloproteinase (MMP) family. This protein is involved in the degradation of components of the extracellular matrix. MMP10 weighs around 54 kDa in its native form. It gets expressed in various tissues including the skin lung and reproductive organs and plays a part in processes like tissue remodeling and repair. Unlike other MMPs MMP10 does not usually cleave collagen but breaks down proteins like elastin and gelatin. The Luminex MMP assay is a method used to study this protein's expression and regulation.
Biological function summary

MMP10 impacts the restructuring and turnover of the extracellular matrix in both normal and disease conditions. It does not work alone; it operates synergistically with other MMPs in a complex matrix framework. Within tissues MMP10 modifies the integrity of the extracellular environment affecting cell behaviors such as migration and invasion. It steps in during processes like wound healing and inflammation exemplifying its contribution to cellular repair mechanisms and their regulation.

Pathways

The activity of MMP10 intersects with the MAPK and TGF-beta signaling pathways. MMP10 facilitates these pathways by digesting extracellular matrix barriers which then influences cellular responses. MMP10's function is also linked with other metalloproteinases like MMP1 and MMP3 in the pathways. Its regulation and activation play an important role in maintaining cellular homeostasis and responding to environmental cues.

MMP10 is associated with conditions such as cancer and arthritis. In cancer its role in promoting extracellular matrix breakdown facilitates tumor cell invasion and metastasis. In arthritis MMP10 contributes to joint tissue destruction perpetuating the inflammatory cycle. This protein frequently collaborates with MMP13 in these diseases reflecting its significant part in disease progression and pathology.
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Product protocols

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Target data

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websiteProtocolBooklet
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