MMP12 Inhibitor Screening Assay Kit (Colorimetric)
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Supplier Data
Functional Studies - MMP12 Inhibitor Screening Assay Kit (Colorimetric) (AB139441)
Example graph for Km and Vmax determination.
Activity of a control sample =
(4.85E-03OD/min x 1E-04L)/(13,600M-1cm-1 x 0.5cm)=
7.13E-11 mol/min at 37°C, 100&mirco;M thiopeptolide
- FuncS
Supplier Data
Functional Studies - MMP12 Inhibitor Screening Assay Kit (Colorimetric) (AB139441)
Plot of OD vs. time.
Slope=V=4.85E-03 OD/min.
- FuncS
Supplier Data
Functional Studies - MMP12 Inhibitor Screening Assay Kit (Colorimetric) (AB139441)
Inhibition of MMP12 by NNGH (100 nM). Example of inhibitor data.
control slope = 5.08E-03 OD/min
inhibitor slope = 4.82E-04 OD/min
inhibitor % activity remaining = (4.82E-04/5.08E-03) x 100 = 9.49%
- FuncS
Supplier Data
Functional Studies - MMP12 Inhibitor Screening Assay Kit (Colorimetric) (AB139441)
Inhibition of MMP12 by NNGH (100 nM)
Reactivity data
Product details
Abcam's MMP12 Inhibitor Screening Assay Kit (Colorimetric) (ab139441) is a complete assay system designed to screen MMP12 inhibitors using a thiopeptide as a chromogenic substrate (Ac-PLG-[2-mercapto-4-methyl-pentanoyl]-LG-OC2H5). The MMP cleavage site peptide bond is replaced by a thioester bond in the thiopeptide. Hydrolysis of this bond by an MMP produces a sulfhydryl group, which reacts with DTNB [5,5'-dithiobis(2-nitrobenzoic acid), Ellman's reagent] to form 2-nitro-5-thiobenzoic acid, which can be detected by its absorbance at 412 nm (e=13,600 M-1cm-1 at pH 6.0 and above). The assays are performed in a convenient 96-well microplate format.
This kit is useful to screen inhibitors of MMP12, a potential therapeutic target. The MMP inhibitor NNGH is also included as a prototypic control inhibitor.
Thiol inhibitors should not be used with this kit, as they may interfere with the colorimetric assay.
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Properties and storage information
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MMP12 plays an important role in tissue remodeling and repair processes. It interacts with other proteases within the extracellular matrix. The degradation of elastin by MMP12 contributes to structural organization and it participates in immune response modulation. As part of the matrix metalloproteinase family MMP12 takes part in the larger proteolytic system which comprises multiple enzymes working in concert.
Pathways
MMP12 is an essential factor in the extracellular matrix degradation pathway. This pathway regulates tissue remodeling and inflammatory response. MMP12 independently breaks down elastin while it interacts with proteins like MMP2 and MMP9 which assist in remodeling processes. It also contributes to the regulation of cytokines that orchestrate immune functions.
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