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AB139442

MMP12 Inhibitor Screening Assay Kit (Fluorometric)

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Abcam MMP12 Inhibitor Screening Assay Kit (Fluorometric) (ab139442) Kit is a complete assay system designed to screen MMP12 inhibitors using a quenched fluorogenic peptide: MMP Fluorogenic Substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 [Mca = (7-methoxycoumarin-4-yl)-acetyl; Dpa=N-3-(2,4-dinitrophenyl)-L-α-β-diaminopropionyl].
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Functional Studies - MMP12 Inhibitor Screening Assay Kit (Fluorometric) (AB139442)
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Functional Studies - MMP12 Inhibitor Screening Assay Kit (Fluorometric) (AB139442)

Inhibition of MMP12 by NNGH (100 nM). Example of inhibitor data.

Key facts

Detection method

Fluorescent

Sample types

Inhibitor compounds

Assay type

Enzyme activity

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam MMP12 Inhibitor Screening Assay Kit (Fluorometric) (ab139442) Kit is a complete assay system designed to screen MMP12 inhibitors using a quenched fluorogenic peptide: MMP Fluorogenic Substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 [Mca = (7-methoxycoumarin-4-yl)-acetyl; Dpa=N-3-(2,4-dinitrophenyl)-L-α-ß-diaminopropionyl]. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties. The assays are performed in a convenient 96-well microplate format.

The kit is useful to screen inhibitors of MMP12, a potential therapeutic target. The compound NNGH is also included as a prototypic control inhibitor.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab139442-1x96Tests", "assetComponentDetails": [ { "size":"1 x 14 µL", "name":"MMP12 Enzyme (Human, Recombinant)", "number":"AB139442-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"96-well White Microplate 1/2 Volume", "number":"AB139442-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"Fluorometric Assay Buffer", "number":"AB139442-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"MMP Fluorogenic Substrate", "number":"AB139442-CMP03", "productcode":"" }, { "size":"1 x 50 µL", "name":"MMP Inhibitor", "number":"AB139442-CMP04", "productcode":"" }, { "size":"1 x 50 µL", "name":"MMP Calibration Standard", "number":"AB139442-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Matrix metallopeptidase 12 commonly known as MMP12 or macrophage elastase is an enzyme with a molecular mass of approximately 54 kDa. Found mainly in macrophages MMP12 belongs to the matrix metallopeptidase family. It functions to break down extracellular matrix proteins specifically elastin. Besides macrophages it sees expression in other tissues including the lungs and skin but it holds particular importance in immune cell-related environments.
Biological function summary

MMP12 plays an important role in tissue remodeling and repair processes. It interacts with other proteases within the extracellular matrix. The degradation of elastin by MMP12 contributes to structural organization and it participates in immune response modulation. As part of the matrix metalloproteinase family MMP12 takes part in the larger proteolytic system which comprises multiple enzymes working in concert.

Pathways

MMP12 is an essential factor in the extracellular matrix degradation pathway. This pathway regulates tissue remodeling and inflammatory response. MMP12 independently breaks down elastin while it interacts with proteins like MMP2 and MMP9 which assist in remodeling processes. It also contributes to the regulation of cytokines that orchestrate immune functions.

Overexpression of MMP12 is strongly linked to chronic obstructive pulmonary disease (COPD) and atherosclerosis. In COPD MMP12's breakdown of elastin contributes to tissue damage in the lung. In atherosclerosis the enzyme accelerates plaque destabilization impacting cardiovascular health. Additionally MMP12 shares functional relationships with proteins such as TIMPs (tissue inhibitors of metalloproteinases) which regulate its activity and play roles in disease pathogenesis.
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Product protocols

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Target data

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websiteProtocolBooklet
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