JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB139450

MMP13 Inhibitor Screening Assay Kit (Colorimetric)

Be the first to review this product! Submit a review

|

(3 Publications)

Abcam MMP13 Inhibitor Screening Assay Kit (Colorimetric) (ab139450) is a complete assay system designed to screen MMP13 inhibitors using a thiopeptide as a chromogenic substrate (Ac-PLG-[2-mercapto-4-methyl-pentanoyl]-LG-OC2H5).

View Alternative Names

Collagenase 3, Matrix metalloproteinase-13, MMP-13, MMP13

4 Images
Functional Studies - MMP13 Inhibitor Screening Assay Kit (Colorimetric) (AB139450)
  • FuncS

Supplier Data

Functional Studies - MMP13 Inhibitor Screening Assay Kit (Colorimetric) (AB139450)

Inhibition of MMP13 by NNGH

Functional Studies - MMP13 Inhibitor Screening Assay Kit (Colorimetric) (AB139450)
  • FuncS

Unknown

Functional Studies - MMP13 Inhibitor Screening Assay Kit (Colorimetric) (AB139450)

Inhibition of MMP13 by NNGH.

control slope = 7.00E-03 OD/min

inhibitor (100nM) slope = 4.50E-04 OD/min

inhibitor % activity remaining = (4.50E-04 / 7.00E-03) x 100 = 6.4%

Functional Studies - MMP13 Inhibitor Screening Assay Kit (Colorimetric) (AB139450)
  • FuncS

Supplier Data

Functional Studies - MMP13 Inhibitor Screening Assay Kit (Colorimetric) (AB139450)

Example graph for Km and Vmax determination

Functional Studies - MMP13 Inhibitor Screening Assay Kit (Colorimetric) (AB139450)
  • FuncS

Supplier Data

Functional Studies - MMP13 Inhibitor Screening Assay Kit (Colorimetric) (AB139450)

Plot of OD vs. time.

Slope=V=7.00E-03 OD/min

Key facts

Detection method

Colorimetric

Sample types

Inhibitor compounds

Assay type

Enzyme activity

Assay Platform

Microplate reader

style
left-column

Complementary Products

Assay Kits

AB139449

MMP9 Inhibitor Screening Assay Kit (Fluorometric)

Functional Studies - MMP9 Inhibitor Screening Assay Kit (Fluorometric) (AB139449)

  • 0
  • 0
Assay Kits

AB139446

MMP2 Inhibitor Screening Assay Kit (Colorimetric)

Functional Studies - MMP2 Inhibitor Screening Assay Kit (Colorimetric) (AB139446)

  • 0
  • 0
Assay Kits

AB139457

MMP10 Inhibitor Screening Assay Kit (Fluorometric)

Functional Studies - MMP10 Inhibitor Screening Assay Kit (Fluorometric) (AB139457)

  • 0
  • 0
Assay Kits

AB112146

MMP Activity Assay Kit (Fluorometric - Green)

Functional Studies - MMP Activity Assay Kit (Fluorometric - Green) (AB112146)

  • 4
  • 6
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam MMP13 Inhibitor Screening Assay Kit (Colorimetric) (ab139450) is a complete assay system designed to screen MMP13 inhibitors using a thiopeptide as a chromogenic substrate (Ac-PLG-[2-mercapto-4-methyl-pentanoyl]-LG-OC2H5). The MMP cleavage site peptide bond is replaced by a thioester bond in the thiopeptide. Hydrolysis of this bond by an MMP produces a sulfhydryl group, which reacts with DTNB [5,5'-dithiobis(2-nitrobenzoic acid), Ellman's reagent] to form 2-nitro-5-thiobenzoic acid, which can be detected by its absorbance at 412 nm (ε=13,600 M-1 cm-1 at pH 6.0 and above). The assays are performed in a convenient 96-well microplate format.

This kit is useful to screen inhibitors of MMP13, a potential therapeutic target. The MMP inhibitor NNGH is also included as a prototypic control inhibitor.

Thiol inhibitors should not be used with this kit, as they may interfere with the colorimetric assay.

style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab139450-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"96-well Clear Microplate 1/2 Volume", "number":"AB139450-CMP05", "productcode":"" }, { "size":"1 x 53 µL", "name":"MMP13 Enzyme (Human, Recombinant)", "number":"AB139450-CMP02", "productcode":"" }, { "size":"1 x 50 µL", "name":"MMP Substrate", "number":"AB139450-CMP04", "productcode":"" }, { "size":"1 x 50 µL", "name":"MMP Inhibitor", "number":"AB139450-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"Colorimetric Assay Buffer", "number":"AB139450-CMP01", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Matrix metallopeptidase 13 (MMP-13) also called collagenase 3 is an enzyme with an approximate molecular mass of 54 kDa. It plays a significant role in the breakdown of extracellular matrix components particularly collagen. MMP-13 is expressed in several tissues including cartilage skin and bone. Its expression sees an increase during tissue remodeling and in pathological conditions. MMP-13 also participates in processes like wound healing and embryonic development making it a focal point for understanding tissue dynamics.
Biological function summary

Matrix metallopeptidase 13 contributes extensively to the degradation of collagen type II a major component of cartilage. As a zinc-dependent endopeptidase MMP-13 is part of the MMP family which facilitates the remodeling of the extracellular matrix. MMP-13 is involved in the proteolytic cascade working in conjunction with other MMPs and elastase to mediate tissue repair and turnover. It does not form complexes but acts in concert with other enzymes to execute its physiological functions effectively.

Pathways

Matrix metallopeptidase 13 significance is most noted in the cartilage degradation pathway. It interacts with other MMPs such as MMP-1 and MMP-9 to efficiently break down extracellular matrix components. These interactions highlight its role in the regulation of matrix metalloproteinase activity within the connective tissue degradation pathway. These pathways are key during normal connective tissue remodeling and in pathological processes illustrating the essential roles of MMPs in maintaining tissue homeostasis.

Matrix metallopeptidase 13 has strong associations with osteoarthritis and rheumatoid arthritis. It contributes to cartilage destruction intensifying the progression of these disorders due to its potent collagenolytic activity. Other proteins like MMP-9 and MMP-14 are also involved in these processes potentially amplifying tissue damage in affected joints. Understanding how MMP-13 and related proteins drive these diseases offers potential therapeutic targets for slowing disease progression and enhancing joint health.
style
left-column

Product protocols

style
left-column

Target data

Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.
See full target information MMP13
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of enzyme inhibition and medicinal chemistry 39:2423174 PubMed39513468

2024

Structure optimization and molecular dynamics studies of new tumor-selective -triazines targeting DNA and MMP-10/13 for halting colorectal and secondary liver cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Christine A Morcos,Nesreen S Haiba,Rafik W Bassily,Marwa M Abu-Serie,Amira F El-Yazbi,Omar A Soliman,Sherine N Khattab,Mohamed Teleb

Journal of enzyme inhibition and medicinal chemistry 39:2388209 PubMed39140776

2024

Non-small cell lung cancer sensitisation to platinum chemotherapy via new thiazole-triazole hybrids acting as dual T-type CCB/MMP-9 inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Hassan Gamal,Khadiga A Ismail,A-Mohsen M E Omar,Mohamed Teleb,Marwa M Abu-Serie,Sun Huang,Abdalla S Abdelsattar,Gerald W Zamponi,Hesham Fahmy

Saudi pharmaceutical journal : SPJ : the official 27:446-452 PubMed30976190

2019

A new perspective on evaluation of medicinal plant biological activities: The correlation between phytomics and matrix metalloproteinases activities of some medicinal plants.

Applications

Unspecified application

Species

Unspecified reactive species

Ekrem-Murat Gonulalan,Emirhan Nemutlu,Lutfiye-Omur Demirezer
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com