MMP14 Inhibitor Screening Assay Kit (Colorimetric)
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Abcam MMP14 Inhibitor Screening Assay Kit (Colorimetric) (ab139454) is a complete assay system designed to screen MMP14 inhibitors using a thiopeptide as a chromogenic substrate (Ac-PLG-[2-mercapto-4-methyl-pentanoyl]-LG-OC2H5).
View Alternative Names
Matrix metalloproteinase-14, MMP-14, MMP-X1, Membrane-type matrix metalloproteinase 1, Membrane-type-1 matrix metalloproteinase, MT-MMP 1, MTMMP1, MT1-MMP, MT1MMP, MMP14
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Supplier Data
Functional Studies - MMP14 Inhibitor Screening Assay Kit (Colorimetric) (AB139454)
Plot of OD vs. time.
Slope=V=2.42E-02 OD/min
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Supplier Data
Functional Studies - MMP14 Inhibitor Screening Assay Kit (Colorimetric) (AB139454)
Inhibition of MMP14 by NNGH. Example of inhibitor data.
- FuncS
Supplier Data
Functional Studies - MMP14 Inhibitor Screening Assay Kit (Colorimetric) (AB139454)
Inhibition of MMP14 by NNGH. Example of inhibitor data.
- FuncS
Supplier Data
Functional Studies - MMP14 Inhibitor Screening Assay Kit (Colorimetric) (AB139454)
Example graph for Km and Vmax determination.
Km=456 μM
Vmax=42.8 pmol/sec
Reactivity data
Product details
Abcam MMP14 Inhibitor Screening Assay Kit (Colorimetric) (ab139454) is a complete assay system designed to screen MMP14 inhibitors using a thiopeptide as a chromogenic substrate (Ac-PLG-[2-mercapto-4-methyl-pentanoyl]-LG-OC2H5). The MMP cleavage site peptide bond is replaced by a thioester bond in the thiopeptide. Hydrolysis of this bond by an MMP produces a sulfhydryl group, which reacts with DTNB [5,5'-dithiobis(2-nitrobenzoic acid), Ellman's reagent] to form 2-nitro-5-thiobenzoic acid, which can be detected by its absorbance at 412 nm (ε=13,600 M-1cm-1 at pH 6.0 and above). The assays are performed in a convenient 96-well microplate format.
This kit is useful to screen inhibitors of MMP14, a potential therapeutic target. The MMP inhibitor NNGH is also included as a prototypic control inhibitor.
Thiol inhibitors should not be used with this kit, as they may interfere with the colorimetric assay.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MMP14 influences extracellular matrix degradation enabling cellular invasion and migration. It exists as a part of macromolecular complexes at the cell surface interacting with other metalloproteinases. This interaction assists in the activation of other MMPs such as pro-MMP2 which further amplify matrix remodeling activities. The hinge region of MMP14 lends flexibility allowing it to effectively engage with substrate proteins.
Pathways
MMP14 significantly affects cell signaling and tissue remodeling pathways. One critical pathway involves the activation of pro-MMP2 which is dependent on MMP14's proteolytic activity. MMP14 also plays a role in the regulation of cell proliferation and apoptosis through its interaction with proteins such as TIMP-2. This relationship between MMP14 and other regulatory proteins emphasizes its functional integration within major biological networks.
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