MMP3 Inhibitor Screening Assay Kit (Colorimetric)
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Abcam's MMP3 Inhibitor Screening Assay Kit (Colorimetric) (ab139439) is a complete assay system designed to screen MMP3 inhibitors using a thiopeptide as a chromogenic substrate (Ac-PLG-[2-mercapto-4-methyl-pentanoyl]-LG-OC2H5).
View Alternative Names
STMY1, MMP3, Stromelysin-1, SL-1, Matrix metalloproteinase-3, Transin-1, MMP-3
- FuncS
Supplier Data
Functional Studies - MMP3 Inhibitor Screening Assay Kit (Colorimetric) (AB139439)
Plot of OD vs. time.
Slope = V= 1.185E-02 OD/min
- FuncS
Supplier Data
Functional Studies - MMP3 Inhibitor Screening Assay Kit (Colorimetric) (AB139439)
Example graph for Km and Vmax determination.
Km = 276 μM
Vmax = 10.6 pmol/sec
- FuncS
Supplier Data
Functional Studies - MMP3 Inhibitor Screening Assay Kit (Colorimetric) (AB139439)
Inhibiton of MMP3 by NNGH
- FuncS
Supplier Data
Functional Studies - MMP3 Inhibitor Screening Assay Kit (Colorimetric) (AB139439)
Inhibition of MMP3 by NNGH (1.3µM).
control slope = 1.4E-02 OD/min
inhibitor slope = 1.3E-04 OD/min
inhibitor % activity remaining = (1.3E-04/1.4E-02) x 100 = 0.9%
Reactivity data
Product details
Abcam's MMP3 Inhibitor Screening Assay Kit (Colorimetric) (ab139439) is a complete assay system designed to screen MMP3 inhibitors using a thiopeptide as a chromogenic substrate (Ac-PLG-[2-mercapto-4-methyl-pentanoyl]-LG-OC2H5). The MMP cleavage site peptide bond is replaced by a thioester bond in the thiopeptide. Hydrolysis of this bond by an MMP produces a sulfhydryl group, which reacts with DTNB [5,5'-dithiobis(2-nitrobenzoic acid), Ellman's reagent] to form 2-nitro-5-thiobenzoic acid, which can be detected by its absorbance at 412 nm (ε=13,600 M-1cm-1 at pH 6.0 and above). The assays are performed in a convenient 96-well microplate format
This kit is useful to screen inhibitors of MMP3, a potential therapeutic target. The MMP inhibitor NNGH is also included as a prototypic control inhibitor.
Thiol inhibitors should not be used with this kit, as they may interfere with the colorimetric assay.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MMP3 contributes to multiple physiological and pathological processes. It supports the remodeling of connective tissues and assists in normal developmental processes. MMP3 can work as part of a complex with other MMP proteins which can enhance or inhibit its activity. The balance of MMPs including MMP3 regulates the extracellular matrix turnover and is important for maintaining tissue homeostasis.
Pathways
MMP3 participates in pathways involved in tissue remodeling and inflammatory responses. It is part of cellular pathways regulated by cytokines like interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-alpha). These pathways link MMP3 to other proteins such as MMP1 and MMP9 which perform overlapping and distinct roles in matrix degradation during inflammation and tissue repair.
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