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AB139452

MMP8 Inhibitor Screening Assay Kit (Colorimetric)

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(1 Publication)

Abcam MMP8 Inhibitor Screening Assay Kit (Colorimetric) (ab139452) is a complete assay system designed to screen MMP8 inhibitors using a thiopeptide as a chromogenic substrate (Ac-PLG-[2-mercapto-4-methyl-pentanoyl]-LG-OC2H5).

View Alternative Names

CLG1, MMP8, Neutrophil collagenase, Matrix metalloproteinase-8, PMNL collagenase, MMP-8, PMNL-CL

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Functional Studies - MMP8 Inhibitor Screening Assay Kit (Colorimetric) (AB139452)
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Supplier Data

Functional Studies - MMP8 Inhibitor Screening Assay Kit (Colorimetric) (AB139452)

Inhibition of MMP8 by NNGH

Functional Studies - MMP8 Inhibitor Screening Assay Kit (Colorimetric) (AB139452)
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Supplier Data

Functional Studies - MMP8 Inhibitor Screening Assay Kit (Colorimetric) (AB139452)

Plot of OD vs. time.

Slope = V = 1.39E-02 OD/min

Functional Studies - MMP8 Inhibitor Screening Assay Kit (Colorimetric) (AB139452)
  • FuncS

Supplier Data

Functional Studies - MMP8 Inhibitor Screening Assay Kit (Colorimetric) (AB139452)

Inhibition of MMP8 by NNGH.

control slope = 1.39E-02 OD/min

inhibitor slope (100nM) = 2.73E-03 OD/min

inhibitor % activity remaining = (2.73E-03/1.39E-02) x 100 = 19.6%

Functional Studies - MMP8 Inhibitor Screening Assay Kit (Colorimetric) (AB139452)
  • FuncS

Supplier Data

Functional Studies - MMP8 Inhibitor Screening Assay Kit (Colorimetric) (AB139452)

Example graph for Km and Vmax determination.

Km = 185 μM

Vmax = 8.90 pmol/sec

Key facts

Detection method

Colorimetric

Sample types

Inhibitor compounds

Assay type

Enzyme activity

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam MMP8 Inhibitor Screening Assay Kit (Colorimetric) (ab139452) is a complete assay system designed to screen MMP8 inhibitors using a thiopeptide as a chromogenic substrate (Ac-PLG-[2-mercapto-4-methyl-pentanoyl]-LG-OC2H5). The MMP cleavage site peptide bond is replaced by a thioester bond in the thiopeptide. Hydrolysis of this bond by an MMP produces a sulfhydryl group, which reacts with DTNB [5,5'-dithiobis(2-nitrobenzoic acid), Ellman's reagent] to form 2-nitro-5-thiobenzoic acid, which can be detected by its absorbance at 412 nm (e=13,600 M-1cm-1 at pH 6.0 and above). The assays are performed in a convenient 96-well microplate format.

This kit is useful to screen inhibitors of MMP8, a potential therapeutic target. The MMP inhibitor NNGH is also included as a prototypic control inhibitor.

Thiol inhibitors should not be used with this kit, as they may interfere with the colorimetric assay.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab139452-1x96Tests", "assetComponentDetails": [ { "size":"1 x 22 µL", "name":"MMP8 Enzyme (Human, Recombinant)", "number":"AB139452-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"96-well Clear Microplate (1/2 Volume)", "number":"AB139452-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"Colorimetric Assay Buffer", "number":"AB139452-CMP01", "productcode":"" }, { "size":"1 x 50 µL", "name":"MMP Substrate", "number":"AB139452-CMP04", "productcode":"" }, { "size":"1 x 50 µL", "name":"MMP Inhibitor", "number":"AB139452-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Matrix metalloproteinase-8 (MMP-8) also known as neutrophil collagenase is an enzyme with a molecular weight of approximately 57 kDa. It belongs to the matrix metalloproteinase family which is involved in the breakdown of extracellular matrix components. MMP-8 mainly expresses in neutrophils but can also be found in other tissues such as the skin lungs and liver. The expression of MMP-8 increases during inflammation and infection where it plays a critical role in tissue remodeling and repair processes.
Biological function summary

MMP-8 participates in the degradation of collagen types I II and III contributing to extracellular matrix remodeling. It acts independently rather than being part of a larger enzyme complex. Its activity is tightly regulated by tissue inhibitors of metalloproteinases (TIMPs) to prevent excessive tissue damage. By modulating the extracellular matrix MMP-8 influences processes like wound healing cell migration and angiogenesis indicating its essential role in maintaining tissue homeostasis.

Pathways

MMP-8 plays a role in the inflammatory response and wound healing pathways. It interacts with and processes other proteins like pro-inflammatory cytokines and growth factors which are important for regulating these pathways. MMP-8 collaborates with matrix metalloproteinase-9 (MMP-9) in the inflammatory pathway to facilitate leukocyte migration and rapid tissue remodeling during acute inflammatory responses. Its regulation involves complex interactions among proteolytic enzymes emphasizing its function in balancing matrix degradation and formation.

MMP-8 is associated with periodontal disease and rheumatoid arthritis. In periodontal disease elevated MMP-8 levels can lead to breakdown of connective tissue and bone contributing to tooth loss. In rheumatoid arthritis MMP-8 alongside MMP-1 degrades joint cartilage collagen exacerbating the inflammation and joint damage. These associations underline MMP-8's potential as a biomarker for these diseases and highlight the need for MMP-8 testing tools such as MMP8 ELISA kits to monitor disease progression and treatment efficacy.
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Product protocols

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Target data

Can degrade fibrillar type I, II, and III collagens.
See full target information MMP8
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Saudi pharmaceutical journal : SPJ : the official 27:446-452 PubMed30976190

2019

A new perspective on evaluation of medicinal plant biological activities: The correlation between phytomics and matrix metalloproteinases activities of some medicinal plants.

Applications

Unspecified application

Species

Unspecified reactive species

Ekrem-Murat Gonulalan,Emirhan Nemutlu,Lutfiye-Omur Demirezer
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