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AB139448

MMP9 Inhibitor Screening Assay Kit (Colorimetric)

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(5 Publications)

MMP9 Inhibitor Screening Assay Kit (Colorimetric) ab139448 is a complete assay system designed to screen MMP9 inhibitors using a thiopeptide as a chromogenic substrate (Ac-PLG-[2-mercapto-4-methyl-pentanoyl]-LG-OC2H5).

View Alternative Names

CLG4B, MMP9, Matrix metalloproteinase-9, MMP-9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB

4 Images
Functional Studies - MMP9 Inhibitor Screening Assay Kit (Colorimetric) (AB139448)
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Supplier Data

Functional Studies - MMP9 Inhibitor Screening Assay Kit (Colorimetric) (AB139448)

Example graph for Km and Vmax determination.

Km=56.1 μM

Vmax=1.8 pmol/sec

Functional Studies - MMP9 Inhibitor Screening Assay Kit (Colorimetric) (AB139448)
  • FuncS

Supplier Data

Functional Studies - MMP9 Inhibitor Screening Assay Kit (Colorimetric) (AB139448)

Inhibition of MMP9 by NNGH.

Control slope = 4.69E-03 OD/min

Inhibitor slope = 2.04E-04 OD/min

Inhibitor % activity remaining = (2.04E-04/4.69E-03) x 100 = 4.34%

Functional Studies - MMP9 Inhibitor Screening Assay Kit (Colorimetric) (AB139448)
  • FuncS

Supplier Data

Functional Studies - MMP9 Inhibitor Screening Assay Kit (Colorimetric) (AB139448)

Plot of OD vs time

Functional Studies - MMP9 Inhibitor Screening Assay Kit (Colorimetric) (AB139448)
  • FuncS

Supplier Data

Functional Studies - MMP9 Inhibitor Screening Assay Kit (Colorimetric) (AB139448)

Inhibition of MMP9 by NNGH

Key facts

Detection method

Colorimetric

Sample types

Inhibitor compounds

Assay type

Enzyme activity

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

MMP9 Inhibitor Screening Assay Kit (Colorimetric) (ab139448) is a complete assay system designed to screen MMP9 inhibitors using a thiopeptide as a chromogenic substrate (Ac-PLG-[2-mercapto-4-methyl-pentanoyl]-LG-OC2H5). The MMP cleavage site peptide bond is replaced by a thioester bond in the thiopeptide. Hydrolysis of this bond by an MMP produces a sulfhydryl group, which reacts with DTNB [5,5'-dithiobis(2-nitrobenzoic acid), Ellman's reagent] to form 2-nitro-5-thiobenzoic acid, which can be detected by its absorbance at 412 nm (e=13,600 M-1cm-1 at pH 6.0 and above). The assays are performed in a convenient 96-well microplate format.

This kit is useful to screen inhibitors of MMP9, a potential therapeutic target. The MMP inhibitor NNGH is also included as a prototypic control inhibitor.

Thiol inhibitors should not be used with this kit, as they may interfere with the colorimetric assay.

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab139448-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"96-well Clear Microplate (1/2 Volume)", "number":"AB139448-CMP05", "productcode":"" }, { "size":"1 x 48.5 µL", "name":"MMP9 Enzyme (Human, Recombinant)", "number":"AB139448-CMP02", "productcode":"" }, { "size":"1 x 50 µL", "name":"MMP Substrate", "number":"AB139448-CMP04", "productcode":"" }, { "size":"1 x 50 µL", "name":"MMP Inhibitor", "number":"AB139448-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"Colorimetric Assay Buffer", "number":"AB139448-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MMP-9 protein also known as matrix metalloproteinase-9 or gelatinase B functions as an enzyme involved in the breakdown of extracellular matrix proteins. It consists of a zinc ion at its active site and facilitates the degradation of type IV and V collagens fibronectin and elastin. MMP-9 has a molecular weight of approximately 92 kDa and is expressed in a variety of tissues including the liver lungs and immune cells. This enzyme can exist in monomer and dimer forms with its activity regulated by tissue inhibitors of metalloproteinases (TIMPs).
Biological function summary

Matrix metalloproteinase-9 profoundly influences tissue remodeling inflammation and angiogenesis. It does not form part of a larger protein complex but interacts closely with substrates in the extracellular matrix. MMP-9 plays important roles in processes such as embryogenesis reproduction and wound healing through the remodeling of surrounding tissues. The MMP-9 protein is often measured using assays like ELISA or analyzed via techniques such as Western blotting to determine its expression levels and activity in different biological contexts.

Pathways

MMP-9 participates in the matrix metalloproteinase pathway and the NF-kB signaling pathway. These pathways are essential in orchestrating processes such as tissue remodeling and inflammatory responses. MMP-9 acts alongside other matrix metalloproteinases including MMP-2 and is regulated by factors like cytokines growth factors and hormones ensuring the precision of extracellular matrix degradation during physiological processes.

Matrix metalloproteinase-9 has associations with cancer invasion and metastasis as well as chronic inflammatory diseases like rheumatoid arthritis. In cancer upregulated MMP-9 expression aids tumor cells in breaching the basement membrane facilitating metastasis. The disorder-associated pathways often involve interactions with proteins like vascular endothelial growth factor (VEGF) and transforming growth factor-beta (TGF-beta). Understanding MMP-9's role in these conditions can contribute to developing targeted therapies that inhibit its activity potentially mitigating disease progression.
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Product protocols

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Target data

Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration (PubMed : 12879005, PubMed : 1480034, PubMed : 2551898). Could play a role in bone osteoclastic resorption (By similarity). Cleaves KiSS1 at a Gly-|-Leu bond (PubMed : 12879005). Cleaves NINJ1 to generate the Secreted ninjurin-1 form (PubMed : 32883094). Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (PubMed : 1480034). Degrades fibronectin but not laminin or Pz-peptide.
See full target information MMP9
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of enzyme inhibition and medicinal chemistry 39:2423174 PubMed39513468

2024

Structure optimization and molecular dynamics studies of new tumor-selective -triazines targeting DNA and MMP-10/13 for halting colorectal and secondary liver cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Christine A Morcos,Nesreen S Haiba,Rafik W Bassily,Marwa M Abu-Serie,Amira F El-Yazbi,Omar A Soliman,Sherine N Khattab,Mohamed Teleb

Journal of enzyme inhibition and medicinal chemistry 39:2388209 PubMed39140776

2024

Non-small cell lung cancer sensitisation to platinum chemotherapy via new thiazole-triazole hybrids acting as dual T-type CCB/MMP-9 inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Hassan Gamal,Khadiga A Ismail,A-Mohsen M E Omar,Mohamed Teleb,Marwa M Abu-Serie,Sun Huang,Abdalla S Abdelsattar,Gerald W Zamponi,Hesham Fahmy

Nanomedicine (London, England) 19:979-994 PubMed38578787

2024

Synergistic eradicating impact of 5-fluouracil with FeO nanoparticles-diethyldithiocarbamate in colon cancer spheroids.

Applications

Unspecified application

Species

Unspecified reactive species

Marwa M Abu-Serie

International journal of molecular sciences 22: PubMed34638665

2021

Halting Tumor Progression via Novel Non-Hydroxamate Triazole-Based Mannich Bases MMP-2/9 Inhibitors; Design, Microwave-Assisted Synthesis, and Biological Evaluation.

Applications

Unspecified application

Species

Unspecified reactive species

Fawzia Faleh Albelwi,Mohamed Teleb,Marwa M Abu-Serie,Mohamed Nabil Abd Al Moaty,Mai S Alsubaie,Mohamed A Zakaria,Yeldez El Kilany,Mohamed Reda Aouad,Mohamed Hagar,Nadjet Rezki

Biomolecules 10: PubMed32784891

2020

Discovery of Sulfated Small Molecule Inhibitors of Matrix Metalloproteinase-8.

Applications

Unspecified application

Species

Unspecified reactive species

Shravan Morla,Umesh R Desai
View all publications
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