Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric) ab273326 provides a quick, sensitive and easy way for measuring MAGL activity in various samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Nature communications 13:69412022The endocannabinoid anandamide is an airway relaxant in health and disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnnika Simon,Thomas von Einem,Alexander Seidinger,Michaela Matthey,Laura Bindila,Daniela WenzelPubMed 36396957
- Frontiers in pharmacology 13:9445022022Pristimerin, a triterpene that inhibits monoacylglycerol lipase activity, prevents the development of paclitaxel-induced allodynia in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAltaf Al-Romaiyan,Willias MasochaPubMed 36016571
Key facts
- Sample types
Adherent cells, Suspension cells, Tissue
Target data
Function
Converts monoacylglycerides to free fatty acids and glycerol (PubMed:19029917, PubMed:20079333, PubMed:21049984, PubMed:22969151, PubMed:24368842). Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain (PubMed:19029917, PubMed:20079333, PubMed:21049984, PubMed:22969151, PubMed:24368842). Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth (PubMed:20079333).
Alternative names
Monoglyceride lipase, MGL, HU-K5, Lysophospholipase homolog, Lysophospholipase-like, Monoacylglycerol lipase, MAGL, MGLL
What's included?
Recommended products
Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric) ab273326 provides a quick, sensitive and easy way for measuring MAGL activity in various samples.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Alternative names
Monoglyceride lipase, MGL, HU-K5, Lysophospholipase homolog, Lysophospholipase-like, Monoacylglycerol lipase, MAGL, MGLL
Key facts
- Sample types
Adherent cells, Suspension cells, Tissue
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric) (ab273326) provides a quick, sensitive and easy way for measuring MAGL activity in various samples. In this assay, a fluorescent substrate is cleaved to generate arachidonic acid and fluorescent metabolite and the increased fluorescence is measured at Ex/Em 360/460 nm. To identify the signal generated specifically by MAGL, a specific inhibitor is included that allows the user to differentiate MAGL activity from other sources of fluorescence.
The assay is simple to perform, high throughput adaptable and can detect as low as 0.1 mU of MAGL activity.
This product is manufactured by BioVision, an Abcam company and was previously called K561 Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric). K561-100 is the same size as the 100 test size of ab273326.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Monoacylglycerol lipase (MGL) also known as MAGL or lipase 63 is an enzyme with a mass of approximately 33 kDa. MGL primarily functions in lipid metabolism by hydrolyzing 2-monoacylglycerol (2-MAG) into free fatty acids and glycerol. This enzyme is expressed in many tissues with high expression in the brain liver and adipose tissue. Its activity plays a significant role in the breakdown of monoacylglycerol an important intermediate in the endocannabinoid signaling system.
- Biological function summary
MGL contributes to the regulation of energy homeostasis by controlling the levels of 2-monoacylglycerol. It catalyzes the final step in the degradation of the endocannabinoid 2-arachidonoylglycerol (2-AG) which is important for modulating inflammation pain and mood. MGL is not known to be part of a larger protein complex. In the brain its action helps terminate endocannabinoid signaling therefore influencing neurotransmission and synaptic plasticity.
- Pathways
MGL plays a critical role in the endocannabinoid and glycerolipid metabolism pathways. In the endocannabinoid system it interacts with the cannabinoid receptors CB1 and CB2 by regulating 2-AG levels. The protein's role in glycerolipid metabolism involves its interaction with long-chain acyl-CoA synthetase and fatty acid oxidation pathways which are essential for maintaining lipid balance and cellular energy.
- Associated diseases and disorders
MGL has been linked to neurodegenerative diseases and cancer. In neurodegenerative diseases like Alzheimer’s dysregulation of MGL activity may lead to altered endocannabinoid signaling impacting neuronal health. In cancer increased MGL activity can promote tumor progression by providing cancer cells with fatty acids for growth and survival. MGL's interaction with proteins such as fatty acid synthase highlights its importance in cancer metabolism.
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3 product images
Functional Studies - Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric) (ab273326)
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Fluorescent standard curve.
Functional Studies - Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric) (ab273326)
Kinetic reactions of MAGL.
Reaction kinetics of Monoacylglycerol Lipase (MAGL) positive control and MAGL activity in rat midbrain lysate (6 μg protein) using appropriate background controls.
Functional Studies - Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric) (ab273326)
Monoacylglycerol Lipase specific activity was calculated in rat midbrain and rat intestinal lysates.
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