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AB273326

Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric)

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(3 Publications)

Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric) ab273326 provides a quick, sensitive and easy method to quantify MAGL enzyme activity.

- Kit includes inhibitor and MAGL enzyme positive control
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

Monoglyceride lipase, MGL, HU-K5, Lysophospholipase homolog, Lysophospholipase-like, Monoacylglycerol lipase, MAGL, MGLL

3 Images
Functional Studies - Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric) (AB273326)
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Supplier Data

Functional Studies - Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric) (AB273326)

Kinetic reactions of MAGL.

Reaction kinetics of Monoacylglycerol Lipase (MAGL) positive control and MAGL activity in rat midbrain lysate (6 μg protein) using appropriate background controls.

Functional Studies - Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric) (AB273326)
  • FuncS

Supplier Data

Functional Studies - Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric) (AB273326)

Monoacylglycerol Lipase specific activity was calculated in rat midbrain and rat intestinal lysates.

Functional Studies - Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric) (AB273326)
  • FuncS

Supplier Data

Functional Studies - Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric) (AB273326)

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Fluorescent standard curve.

Key facts

Sample types

Adherent cells, Suspension cells, Tissue

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric) ab273326 provides a quick, sensitive and easy method for measuring MAGL activity.

Monoacylglycerol Lipase (MAGL) assay principle
In the MAGL assay, a fluorescent substrate is cleaved to generate arachidonic acid and a fluorescent dye. The increase in fluorescence is then measured at Ex/Em 360/460 nm using a fluorometric plate reader. To identify the signal generated specifically by MAGL, a specific inhibitor is included that allows the user to differentiate MAGL activity from other sources of fluorescence.

Monoacylglycerol Lipase (MAGL) assay protocol summary
- Prepare all samples, controls and standards.
- add reaction mix to each well
- incubate for 20-30 min at 37°C
- measure fluorescence in kinetic mode for 60 mins at 37°C

Other notes
This product was previously called K561 Biovision Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric). Abcam acquired Biovision in 2021.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab273326-100Test", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"Mutant IDH Lysis Buffer", "number":"AB273326-CMP01", "productcode":"AB323979" }, { "size":"1 x 1 Vial", "name":"MAGL Positive Control", "number":"AB273326-CMP02", "productcode":"" }, { "size":"1 x 100 µL", "name":"MAGL Inhibitor", "number":"AB273326-CMP03", "productcode":"" }, { "size":"1 x 40 µL", "name":"Umbelliferone Standard", "number":"AB273326-CMP05", "productcode":"" }, { "size":"1 x 50 µL", "name":"MAGL Substrate", "number":"AB273326-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Monoacylglycerol lipase (MGL) also known as MAGL or lipase 63 is an enzyme with a mass of approximately 33 kDa. MGL primarily functions in lipid metabolism by hydrolyzing 2-monoacylglycerol (2-MAG) into free fatty acids and glycerol. This enzyme is expressed in many tissues with high expression in the brain liver and adipose tissue. Its activity plays a significant role in the breakdown of monoacylglycerol an important intermediate in the endocannabinoid signaling system.
Biological function summary

MGL contributes to the regulation of energy homeostasis by controlling the levels of 2-monoacylglycerol. It catalyzes the final step in the degradation of the endocannabinoid 2-arachidonoylglycerol (2-AG) which is important for modulating inflammation pain and mood. MGL is not known to be part of a larger protein complex. In the brain its action helps terminate endocannabinoid signaling therefore influencing neurotransmission and synaptic plasticity.

Pathways

MGL plays a critical role in the endocannabinoid and glycerolipid metabolism pathways. In the endocannabinoid system it interacts with the cannabinoid receptors CB1 and CB2 by regulating 2-AG levels. The protein's role in glycerolipid metabolism involves its interaction with long-chain acyl-CoA synthetase and fatty acid oxidation pathways which are essential for maintaining lipid balance and cellular energy.

MGL has been linked to neurodegenerative diseases and cancer. In neurodegenerative diseases like Alzheimer's dysregulation of MGL activity may lead to altered endocannabinoid signaling impacting neuronal health. In cancer increased MGL activity can promote tumor progression by providing cancer cells with fatty acids for growth and survival. MGL's interaction with proteins such as fatty acid synthase highlights its importance in cancer metabolism.
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Product protocols

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Target data

Converts monoacylglycerides to free fatty acids and glycerol (PubMed : 19029917, PubMed : 20079333, PubMed : 21049984, PubMed : 22969151, PubMed : 24368842). Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain (PubMed : 19029917, PubMed : 20079333, PubMed : 21049984, PubMed : 22969151, PubMed : 24368842). Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth (PubMed : 20079333).
See full target information MGLL
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Neuroscience 594:1-10 PubMed41397672

2025

Monoacylglycerol lipase inhibition improves motor function and reduces oxidative stress in hemiparkinsonian mice.

Applications

Unspecified application

Species

Unspecified reactive species

Delia Maciel Mendoza-Camacho,Juan Manuel Viveros-Paredes,Mario Eduardo Flores-Soto,Alma Hortensia Martínez-Preciado,Aldo Rafael Tejeda-Martínez

Nature communications 13:6941 PubMed36396957

2022

The endocannabinoid anandamide is an airway relaxant in health and disease.

Applications

Unspecified application

Species

Unspecified reactive species

Annika Simon,Thomas von Einem,Alexander Seidinger,Michaela Matthey,Laura Bindila,Daniela Wenzel

Frontiers in pharmacology 13:944502 PubMed36016571

2022

Pristimerin, a triterpene that inhibits monoacylglycerol lipase activity, prevents the development of paclitaxel-induced allodynia in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Altaf Al-Romaiyan,Willias Masocha
View all publications
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Product promise

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