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AB284510

Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit (Fluorometric)

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(2 Publications)

Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit ab284510 offers a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of MAO-A inhibitors.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

Amine oxidase [flavin-containing] A, Monoamine oxidase type A, MAO-A, MAOA

3 Images
Functional Studies - Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit (Fluorometric) (AB284510)
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Supplier Data

Functional Studies - Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit (Fluorometric) (AB284510)

Monoamine Oxidase A (MOA-A) Inhibitor Screening Kit.

Clorgyline does not inhibit MAO-B activity (an isozyme of MAO-A).

Functional Studies - Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit (Fluorometric) (AB284510)
  • FuncS

Supplier Data

Functional Studies - Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit (Fluorometric) (AB284510)

Monoamine Oxidase A (MOA-A) Inhibitor Screening Kit.

Inhibition of MAO-A Activity with Clorgyline.

Other - Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit (Fluorometric) (AB284510)
  • Other

Supplier Data

Other - Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit (Fluorometric) (AB284510)

Representative image of Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit (Fluorometric) ab284510

Components shown from left to right :

- OxiRed Probe

- MAO Substrate

- Developer Solution V

- MAO-A Enzyme

- MAO-A Inhibitor

- Assay Buffer 18

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Fluorescent

Sample types

Inhibitor compounds

Assay type

Enzyme activity

Assay Platform

Microplate (12 x 8 well strips)

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Complementary Products

Assay Kits

AB241031

Monoamine Oxidase (MAO) Assay Kit

Functional Studies - Monoamine Oxidase (MAO) Assay Kit (AB241031)

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Assay Kits

AB284511

Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (Fluorometric)

Functional Studies - Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (Fluorometric) (AB284511)

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  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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  • 20
Primary Antibodies

AB126751

Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase A/MAO-A antibody [EPR7101] (AB126751)

  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit (ab284510) (K796) offers a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of MAO-A inhibitors.

The assay is based on the fluorometric detection of H2O2, one of the byproducts generated during the oxidative deamination of MAO substrate (Tyramine).

Related MAO assay kits
Use this kit Monoamine Oxidase (MAO) Assay Kit ab241031 to measure total MAO, MAO-A and MAO-B activity in biological samples.
Use Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit (Fluorometric) ab284510, and Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (Fluorometric) ab284511 to assay MAO inhibitors with purified MAO A and MAO B enzymes

Other notes
This product was previously called K796 Biovision Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit (Fluorometric). Biovision was acquired by Abcam in 2021.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab284510-100Test", "assetComponentDetails": [ { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB284510-CMP03" }, { "size":"1 x 1 Vial", "name":"MAO-A Inhibitor", "number":"AB284510-CMP02" }, { "size":"1 x 1 Vial", "name":"MAO-A Enzyme", "number":"AB284510-CMP05" }, { "size":"1 x 1 Vial", "name":"MAO Substrate", "number":"AB284510-CMP06" }, { "size":"1 x 1 Vial", "name":"Developer Solution V", "number":"AB284510-CMP01" }, { "size":"1 x 25 mL", "name":"Assay Buffer 18", "number":"AB284510-CMP04" } ] }, "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab284510-100Test", "assetComponentDetails": [ { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB284510-CMP03", "productcode":"AB309437" }, { "size":"1 x 1 Vial", "name":"MAO-A Inhibitor", "number":"AB284510-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"MAO-A Enzyme", "number":"AB284510-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"MAO Substrate", "number":"AB284510-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Solution V", "number":"AB284510-CMP01", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 18", "number":"AB284510-CMP04", "productcode":"AB323954" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Monoamine Oxidase A (MAO-A) also known as MAO protein is an enzyme that degrades neurotransmitters through oxidative deamination. It possesses a mass of approximately 59 kDa. This enzyme is found localized in the outer mitochondrial membrane of cells specifically in high concentrations in the brain liver and intestine. MAO-A plays an important role in the breakdown of monoamine neurotransmitters such as serotonin norepinephrine and dopamine which are critical for normal brain function.
Biological function summary

MAO-A acts to maintain proper neurotransmitter levels by catalyzing the oxidative deamination of monoamines. It is not part of a larger protein complex but functions independently to influence monoamine balance. Through its enzymatic activity MAO-A regulates several physiological processes including mood and emotional responses by controlling neurotransmitter availability and preventing their accumulation to neurotoxic levels.

Pathways

MAO-A operates within the catabolic pathways that reduce the bioavailability of monoamines. It pairs with proteins like Catechol-O-methyltransferase (COMT) in the metabolic degradation of catecholamines. This pathway is integral to managing the equilibrium of mood-related neurotransmitters and involves complex interactions with other enzymatic systems including MAO-B which targets different substrates.

MAO-A is closely associated with psychiatric conditions such as depression and anxiety. Altered MAO-A activity can dysregulate monoamine levels contributing to these disorders. Additionally research links MAO-A dysregulation to neurodegenerative diseases like Parkinson's disease where imbalances in monoamine levels play a role. This enzyme interacts with proteins involved in early stages of these diseases highlighting its potential as a target for therapeutic interventions using MAO inhibitors.
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Product protocols

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Target data

Catalyzes the oxidative deamination of primary and some secondary amine such as neurotransmitters, with concomitant reduction of oxygen to hydrogen peroxide and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues (PubMed : 18391214, PubMed : 20493079, PubMed : 24169519, PubMed : 8316221). Preferentially oxidizes serotonin (PubMed : 20493079, PubMed : 24169519). Also catalyzes the oxidative deamination of kynuramine to 3-(2-aminophenyl)-3-oxopropanal that can spontaneously condense to 4-hydroxyquinoline (By similarity).
See full target information MAOA
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

ACS pharmacology & translational science 7:150-160 PubMed38230273

2024

Monoamine Oxidase-A (MAO-A) Inhibitors Screened from the Autodisplayed Fv-Antibody Library.

Applications

Unspecified application

Species

Unspecified reactive species

Jeong Soo Sung,Seunghwan Kim,Jaeyong Jung,Tae-Hun Kim,Soonil Kwon,Hyung Eun Bae,Min-Jung Kang,Joachim Jose,Misu Lee,Jae-Chul Pyun

Chemistry & biodiversity 20:e202301271 PubMed37806964

2023

Benzothiazole-Propanamide Linker Pyrrolidine (Morpholine) as Monoamine Oxidase-B and Butyrylcholinesterase Inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Wen He,B S Kai-Yin Jiang,Xin-Hao Sun,Min Tang,Ya-Wen Liu,Li-Ping Guan,Di Wu
View all publications
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Product promise

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