Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit ab284510 offers a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of MAO-A inhibitors.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Chemistry & biodiversity 20:e2023012712023Benzothiazole-Propanamide Linker Pyrrolidine (Morpholine) as Monoamine Oxidase-B and Butyrylcholinesterase Inhibitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhi-Wen He,B S Kai-Yin Jiang,Xin-Hao Sun,Min Tang,Ya-Wen Liu,Li-Ping Guan,Di WuPubMed 37806964
Key facts
- Detection method
- Fluorescent
- Sample types
- Inhibitor compounds
- Assay type
- Enzyme activity
Target data
Function
Catalyzes the oxidative deamination of primary and some secondary amine such as neurotransmitters, with concomitant reduction of oxygen to hydrogen peroxide and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues (PubMed:18391214, PubMed:20493079, PubMed:24169519, PubMed:8316221). Preferentially oxidizes serotonin (PubMed:20493079, PubMed:24169519). Also catalyzes the oxidative deamination of kynuramine to 3-(2-aminophenyl)-3-oxopropanal that can spontaneously condense to 4-hydroxyquinoline (By similarity).
Alternative names
Amine oxidase [flavin-containing] A, Monoamine oxidase type A, MAO-A, MAOA
What's included?
Recommended products
Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit ab284510 offers a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of MAO-A inhibitors.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
- Fluorescent
- Sample types
- Inhibitor compounds
- Assay type
- Enzyme activity
- Assay Platform
- Microplate (12 x 8 well strips)
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
This Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit (ab284510) (K796) offers a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of MAO-A inhibitors.
The assay is based on the fluorometric detection of H2O2, one of the byproducts generated during the oxidative deamination of MAO substrate (Tyramine).
Related MAO assay kits
Use this kit Monoamine Oxidase (MAO) Assay Kit Monoamine Oxidase (MAO) Assay Kit ab241031 to measure total MAO, MAO-A and MAO-B activity in biological samples.
Use Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit (Fluorometric) ab284510, and Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (Fluorometric) Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (Fluorometric) ab284511 to assay MAO inhibitors with purified MAO A and MAO B enzymes
Other notes
This product was previously called K796 Biovision Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit (Fluorometric). Biovision was acquired by Abcam in 2021.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Monoamine Oxidase A (MAO-A) also known as MAO protein is an enzyme that degrades neurotransmitters through oxidative deamination. It possesses a mass of approximately 59 kDa. This enzyme is found localized in the outer mitochondrial membrane of cells specifically in high concentrations in the brain liver and intestine. MAO-A plays an important role in the breakdown of monoamine neurotransmitters such as serotonin norepinephrine and dopamine which are critical for normal brain function.
- Biological function summary
MAO-A acts to maintain proper neurotransmitter levels by catalyzing the oxidative deamination of monoamines. It is not part of a larger protein complex but functions independently to influence monoamine balance. Through its enzymatic activity MAO-A regulates several physiological processes including mood and emotional responses by controlling neurotransmitter availability and preventing their accumulation to neurotoxic levels.
- Pathways
MAO-A operates within the catabolic pathways that reduce the bioavailability of monoamines. It pairs with proteins like Catechol-O-methyltransferase (COMT) in the metabolic degradation of catecholamines. This pathway is integral to managing the equilibrium of mood-related neurotransmitters and involves complex interactions with other enzymatic systems including MAO-B which targets different substrates.
- Associated diseases and disorders
MAO-A is closely associated with psychiatric conditions such as depression and anxiety. Altered MAO-A activity can dysregulate monoamine levels contributing to these disorders. Additionally research links MAO-A dysregulation to neurodegenerative diseases like Parkinson's disease where imbalances in monoamine levels play a role. This enzyme interacts with proteins involved in early stages of these diseases highlighting its potential as a target for therapeutic interventions using MAO inhibitors.
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2 product images
Functional Studies - Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit (Fluorometric) (ab284510)
Monoamine Oxidase A (MOA-A) Inhibitor Screening Kit.
Inhibition of MAO-A Activity with Clorgyline.
Functional Studies - Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit (Fluorometric) (ab284510)
Monoamine Oxidase A (MOA-A) Inhibitor Screening Kit.
Clorgyline does not inhibit MAO-B activity (an isozyme of MAO-A).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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