Monoamine oxidase B Activity Assay Kit ((MAOB Assay)
Be the first to review this product! Submit a review
|
(1 Publication)
ab109912 (MS747) is a novel assay that uses a high affinity monoclonal capture antibody to selectively isolate MAOB from all other peroxidases and oxidases (including MAOA) in a tissue or cultured cell sample.
View Alternative Names
Amine oxidase [flavin-containing] B, Monoamine oxidase type B, MAO-B, MAOB
- FuncS
Supplier Data
Functional Studies - Monoamine oxidase B Activity Assay Kit ((MAOB Assay) (AB109912)
With a HepG2 cell lysate MAOB activity was clearly measurable in the 16-1000 µg/ mL range and quantity in the range 1-1000 µg/mL. The MAOB specific inhibitor pargyline inhibited activity 90% while not affecting quantity.
- FuncS
Supplier Data
Functional Studies - Monoamine oxidase B Activity Assay Kit ((MAOB Assay) (AB109912)
MAOB is selectively inhibited by selegiline and pargyline, but not clorgyline. In this example, raw data was exported to Graph Pad Prism for 4-parameter fit analysis and IC50 determination.
- Schematic Diagram
Supplier Data
Schematic Diagram - Monoamine oxidase B Activity Assay Kit ((MAOB Assay) (AB109912)
Abcam's protein quantity microplate assays use the well-established sandwich ELISA format, whereby capture and detector antibodies are used to immobilize and then quantify a target protein or enzyme. All of our microplate assays utilize our highly-validated immunocapture antibodies, which are able to capture large, multi-subunit enzyme complexes in their fully intact state. Capture antibodies are pre-coated in the wells of premium Nunc MaxiSorp™ modular microplates, which can be broken into 8-well strips. After the target has been immobilized in the well, a second monoclonal antibody, against a different epitope on the target, is added to the well. This detector antibody is either directly labeled with biotin, or a biotin-labeled goat anti-mouse secondary is added. Substrate plus HRP or AP conjugated to streptavidin provide a colorimetric signal that is readable by any plate readers capable of standard ELISA absorbance measurements.
Reactivity data
Product details
ab109912 (MS747) is a novel assay that uses a high affinity monoclonal capture antibody to selectively isolate MAOB from all other peroxidases and oxidases (including MAOA) in a tissue or cultured cell sample. After isolation and subsequent measurement of the enzyme's functional activity, the quantity of isolated MAOB is measured in the same well by adding a second monoclonal detector antibody, which is quantified using a colorimetric label (HRP). Both reactions take place in time-dependent manners proportional to the amount of enzyme captured in each well. By combining activity and quantity measurements, the enzyme's relative specific activity can be determined. Specific activity is useful for measuring up or down regulation of activity by site-specific modification or damage, and in response to specific inhibitors.
Store Fluorophore and benzylamine at -80°C. Store all other components store at 4°C.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The enzyme contributes to the breakdown of biogenic and dietary amines supporting neurotransmitter regulation and signal modulation. It does not work as part of a larger protein complex but has important interactions within its local cellular environment. MAO-B helps maintain the proper levels of monoamine neurotransmitters preventing their accumulation and potential neurotoxicity.
Pathways
Monoamine Oxidase B functions in the dopamine and serotonin metabolic pathways where it actively participates in the oxidative deamination of monoamines. In these pathways MAO-B acts alongside other proteins such as Monoamine Oxidase A (MAO-A) which shares similar functions and substrate preferences. This enzyme activity helps modulate mood and emotional behaviors which are critical for normal brain function.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Toxicology and applied pharmacology 358:10-22 PubMed30195017
2018
Applications
Unspecified application
Species
Unspecified reactive species
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com