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AB284511

Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (Fluorometric)

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(1 Publication)

This Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (ab284511) (K797) offers a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of MAO-B inhibitors.

View Alternative Names

Amine oxidase [flavin-containing] B, Monoamine oxidase type B, MAO-B, MAOB

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Functional Studies - Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (Fluorometric) (AB284511)
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Supplier Data

Functional Studies - Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (Fluorometric) (AB284511)

Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit.

Inhibition of MAO-B Activity with Selegiline.

Functional Studies - Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (Fluorometric) (AB284511)
  • FuncS

Supplier Data

Functional Studies - Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (Fluorometric) (AB284511)

Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit.

Selegiline does not inhibit MAO-A activity (an isozyme of MAO-B).

Key facts

Detection method

Fluorescent

Sample types

Inhibitor compounds

Assay type

Enzyme activity

Results type

Quantitative

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (ab284511) (K797) offers a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of MAO-B inhibitors.

The assay is based on the fluorometric detection of H2O2, one of the byproducts generated during the oxidative deamination of MAO substrate (Tyramine).

This product is manufactured by BioVision, an Abcam company and was previously called K797 Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (Fluorometric). K797-100 is the same size as the 100 test size of ab284511.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab284511-100Test", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"Assay Buffer 18", "number":"AB284511-CMP04", "productcode":"AB323954" }, { "size":"1 x 1 Vial", "name":"MAO-B Inhibitor", "number":"AB284511-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Solution V", "number":"AB284511-CMP01", "productcode":"" }, { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB284511-CMP03", "productcode":"AB309437" }, { "size":"1 x 1 Vial", "name":"MAO-B Enzyme", "number":"AB284511-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"MAO Substrate", "number":"AB284511-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Monoamine Oxidase B (MAO-B) also known as MAOB or MAO-B inhibitor is an enzyme that is a part of the flavin-containing amine oxidases group. It exists as a homodimer with each monomer having a mass of about 58 kDa. This enzyme plays an important role in the catabolism of monoamines by oxidizing neurotransmitters such as dopamine in the brain. MAO-B is expressed mainly in the outer membrane of mitochondria in neuronal and glial cells and to a lesser extent in other tissues like the liver.
Biological function summary

The enzyme contributes to the breakdown of biogenic and dietary amines supporting neurotransmitter regulation and signal modulation. It does not work as part of a larger protein complex but has important interactions within its local cellular environment. MAO-B helps maintain the proper levels of monoamine neurotransmitters preventing their accumulation and potential neurotoxicity.

Pathways

Monoamine Oxidase B functions in the dopamine and serotonin metabolic pathways where it actively participates in the oxidative deamination of monoamines. In these pathways MAO-B acts alongside other proteins such as Monoamine Oxidase A (MAO-A) which shares similar functions and substrate preferences. This enzyme activity helps modulate mood and emotional behaviors which are critical for normal brain function.

Monoamine Oxidase B has a significant connection with neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. In Parkinson's disease MAO-B intensifies the oxidative stress by promoting dopamine breakdown which is linked to neuronal degeneration. In Alzheimer's disease elevated MAO-B activity may contribute to the overproduction of neurotoxic metabolites. Through these conditions MAO-B is often targeted by MAO-B inhibitors to reduce its activity alleviating symptoms and slowing disease progression.
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Product protocols

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Target data

Catalyzes the oxidative deamination of primary and some secondary amines such as neurotransmitters, and exogenous amines including the tertiary amine, neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), with concomitant reduction of oxygen to hydrogen peroxide and participates in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues (PubMed : 11049757, PubMed : 11134050, PubMed : 20493079, PubMed : 8316221, PubMed : 8665924). Preferentially degrades benzylamine and phenylethylamine (PubMed : 11049757, PubMed : 11134050, PubMed : 20493079, PubMed : 8316221, PubMed : 8665924).
See full target information MAOB
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceuticals (Basel, Switzerland) 17: PubMed39204166

2024

Neuroprotective Effects of Phenolic Constituents from Drynariae Rhizoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Sung Ahn,Chung Hyeon Lee,Xiang-Qian Liu,Kwang Woo Hwang,Mi Hyune Oh,So-Young Park,Wan Kyunn Whang
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websiteProtocolBooklet
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Product promise

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