This Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (ab284511) (K797) offers a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of MAO-B inhibitors.
Images
Key facts
- Detection method
Fluorescent
- Sample types
Inhibitor compounds
- Assay type
Enzyme activity (quantitative)
Target data
Function
Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine.
Alternative names
Amine oxidase [flavin-containing] B, Monoamine oxidase type B, MAO-B, MAOB
What's included?
Recommended products
This Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (ab284511) (K797) offers a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of MAO-B inhibitors.
Alternative names
Amine oxidase [flavin-containing] B, Monoamine oxidase type B, MAO-B, MAOB
Key facts
- Detection method
Fluorescent
- Sample types
Inhibitor compounds
- Assay type
Enzyme activity (quantitative)
- Assay Platform
Microplate (12 x 8 well strips)
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
This Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (ab284511) (K797) offers a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of MAO-B inhibitors.
The assay is based on the fluorometric detection of H2O2, one of the byproducts generated during the oxidative deamination of MAO substrate (Tyramine).
This product is manufactured by BioVision, an Abcam company and was previously called K797 Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (Fluorometric). K797-100 is the same size as the 100 test size of ab284511.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Monoamine Oxidase B (MAO-B) also known as MAOB or MAO-B inhibitor is an enzyme that is a part of the flavin-containing amine oxidases group. It exists as a homodimer with each monomer having a mass of about 58 kDa. This enzyme plays an important role in the catabolism of monoamines by oxidizing neurotransmitters such as dopamine in the brain. MAO-B is expressed mainly in the outer membrane of mitochondria in neuronal and glial cells and to a lesser extent in other tissues like the liver.
- Biological function summary
The enzyme contributes to the breakdown of biogenic and dietary amines supporting neurotransmitter regulation and signal modulation. It does not work as part of a larger protein complex but has important interactions within its local cellular environment. MAO-B helps maintain the proper levels of monoamine neurotransmitters preventing their accumulation and potential neurotoxicity.
- Pathways
Monoamine Oxidase B functions in the dopamine and serotonin metabolic pathways where it actively participates in the oxidative deamination of monoamines. In these pathways MAO-B acts alongside other proteins such as Monoamine Oxidase A (MAO-A) which shares similar functions and substrate preferences. This enzyme activity helps modulate mood and emotional behaviors which are critical for normal brain function.
- Associated diseases and disorders
Monoamine Oxidase B has a significant connection with neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. In Parkinson's disease MAO-B intensifies the oxidative stress by promoting dopamine breakdown which is linked to neuronal degeneration. In Alzheimer's disease elevated MAO-B activity may contribute to the overproduction of neurotoxic metabolites. Through these conditions MAO-B is often targeted by MAO-B inhibitors to reduce its activity alleviating symptoms and slowing disease progression.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Functional Studies - Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (Fluorometric) (ab284511)
Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit.
Inhibition of MAO-B Activity with Selegiline.
Functional Studies - Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (Fluorometric) (ab284511)
Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit.
Selegiline does not inhibit MAO-A activity (an isozyme of MAO-B).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com