Monoamine Oxidase (MAO) Assay Kit (ab241031) is a sensitive assay to detect total monoamine oxidase activity as well as MAO-A and MAO-B isoenzyme activities separately in the presence of Clorgyline and Selegiline - specific inhibitors for MAO-A and MAOB, respectively.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Publications
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- International journal of molecular sciences 24:2023Parkin Precipitates on Mitochondria via Aggregation and Autoubiquitination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMustafa T Ardah,Nada Radwan,Engila Khan,Tohru Kitada,M Emdadul HaquePubMed 37240373
Key facts
- Detection method
Fluorescent
- Sample types
Suspension cells, Adherent cells, Plasma, Tissue Homogenate, Serum
Target data
Function
Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine.
Alternative names
Amine oxidase [flavin-containing] A, Monoamine oxidase type A, MAO-A, MAOA
What's included?
Recommended products
Monoamine Oxidase (MAO) Assay Kit (ab241031) is a sensitive assay to detect total monoamine oxidase activity as well as MAO-A and MAO-B isoenzyme activities separately in the presence of Clorgyline and Selegiline - specific inhibitors for MAO-A and MAOB, respectively.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Alternative names
Amine oxidase [flavin-containing] A, Monoamine oxidase type A, MAO-A, MAOA
Key facts
- Detection method
Fluorescent
- Sample types
Suspension cells, Adherent cells, Plasma, Tissue Homogenate, Serum
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Monoamine Oxidase (MAO) Assay Kit (ab241031) is a sensitive assay to detect total monoamine oxidase activity as well as MAO-A and MAO-B isoenzyme activities separately in the presence of Clorgyline and Selegiline - specific inhibitors for MAO-A and MAOB, respectively. The assay is based on the fluorometric detection of H2O2, one of the by-products generated during the oxidative deamination of the MAO substrate (Tyramine). The assay can detect as little as 5 μU of MAO enzymatic activity.
This product is manufactured by BioVision, an Abcam company and was previously called K795 Monoamine Oxidase Activity (Total MAO/MAO-A/MAO-B) Fluorometric Assay Kit. K795-100 is the same size as the 100 test size of ab241031.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Monoamine oxidase (MAO) also known as MAO-A (with a mass of approximately 59 kDa) and MAO-B is a mitochondrial enzyme that catalyzes the oxidative deamination of monoamines. It primarily exists in the outer mitochondrial membrane and plays a significant role in the metabolism of neurotransmitters such as serotonin dopamine and norepinephrine. MAO enzymes are majorly found in the brain liver and gastrointestinal tract and their activity regulates neurotransmitter levels that influence mood and behavior.
- Biological function summary
MAO activity impacts the regulation of neurotransmitter signaling in the nervous system. MAO does not function as a part of a large complex; it exists as a dimeric protein yet independently performs critical enzymatic tasks. Its primary function is to prevent the accumulation of excessive monoamines which could lead to neurotoxicity and affect cellular homeostasis.
- Pathways
MAO plays an important role in the catecholamine and serotonin signaling pathways. In particular it functions in degrading serotonin and catecholamines affecting neurotransmission balance. Through these pathways MAO interacts with other critical proteins such as catechol-O-methyltransferase (COMT) and dopamine beta-hydroxylase which also participate in the modulation of neurotransmitter activity.
- Associated diseases and disorders
MAO influences several conditions including depression and Parkinson's disease. Alterations in MAO activity can affect levels of serotonin and other neurotransmitters linked to these disorders. Additionally in the context of neurological diseases changes in MAO function can interact with proteins like alpha-synuclein potentially contributing to neurodegenerative processes associated with Parkinson's disease.
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3 product images
Functional Studies - Monoamine Oxidase (MAO) Assay Kit (ab241031)
H2O2 Standard Curve.
H2O2 Standard Curve.
Functional Studies - Monoamine Oxidase (MAO) Assay Kit (ab241031)
MAO-T, MAO-A and MAO-B activities in mouse brain lysate (2.5 µg).
MAO-T, MAO-A and MAO-B activities in mouse brain lysate (2.5 μg).
Functional Studies - Monoamine Oxidase (MAO) Assay Kit (ab241031)
Specific activities of MAO-A and MAO-B. Assays were performed following the kit protocols.
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