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AB241031

Monoamine Oxidase (MAO) Assay Kit

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(6 Publications)

Monoamine Oxidase (MAO) Assay Kit (ab241031) is a sensitive assay to detect total monoamine oxidase activity as well as MAO-A and MAO-B isoenzyme activities separately in the presence of Clorgyline and Selegiline - specific inhibitors for MAO-A and MAOB, respectively.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

View Alternative Names

Amine oxidase [flavin-containing] A, Monoamine oxidase type A, MAO-A, MAOA

3 Images
Functional Studies - Monoamine Oxidase (MAO) Assay Kit (AB241031)
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Supplier Data

Functional Studies - Monoamine Oxidase (MAO) Assay Kit (AB241031)

MAO-T, MAO-A and MAO-B activities in mouse brain lysate (2.5 µg).

MAO-T, MAO-A and MAO-B activities in mouse brain lysate (2.5 μg).

Functional Studies - Monoamine Oxidase (MAO) Assay Kit (AB241031)
  • FuncS

Supplier Data

Functional Studies - Monoamine Oxidase (MAO) Assay Kit (AB241031)

Specific activities of MAO-A and MAO-B. Assays were performed following the kit protocols.

Functional Studies - Monoamine Oxidase (MAO) Assay Kit (AB241031)
  • FuncS

Supplier Data

Functional Studies - Monoamine Oxidase (MAO) Assay Kit (AB241031)

H2O2 Standard Curve.

H2O2 Standard Curve.

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells, Plasma, Tissue Homogenate, Serum

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Assay Kits

AB284510

Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit (Fluorometric)

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AB284511

Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (Fluorometric)

Functional Studies - Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (Fluorometric) (AB284511)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Monoamine Oxidase (MAO) Assay Kit (ab241031) is a sensitive assay to quantify total monoamine oxidase activity. The kit can also be used to measure MAO-A and MAO-B isoenzyme activities separately by using the specific inhibitors for MAO-A (Clorgyline) and MAOB (Selegiline) that are included in the kit.

The assay is based on the fluorometric detection of H2O2, one of the by-products generated during the oxidative deamination of the MAO substrate (Tyramine). The assay can detect as little as 5 μU of MAO enzymatic activity.

Related MAO assay kits
Use this kit Monoamine Oxidase (MAO) Assay Kit ab241031 to measure total MAO, MAO-A and MAO-B activity in biological samples.
Use Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit (Fluorometric) ab284510, and Monoamine Oxidase B (MAO-B) Inhibitor Screening Kit (Fluorometric) ab284511 to assay MAO inhibitors with purified MAO A and MAO B enzymes

Other notes
This product was previously called K795 Biovision Monoamine Oxidase Activity (Total MAO/MAO-A/MAO-B) Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Monoamine oxidase (MAO) also known as MAO-A (with a mass of approximately 59 kDa) and MAO-B is a mitochondrial enzyme that catalyzes the oxidative deamination of monoamines. It primarily exists in the outer mitochondrial membrane and plays a significant role in the metabolism of neurotransmitters such as serotonin dopamine and norepinephrine. MAO enzymes are majorly found in the brain liver and gastrointestinal tract and their activity regulates neurotransmitter levels that influence mood and behavior.
Biological function summary

MAO activity impacts the regulation of neurotransmitter signaling in the nervous system. MAO does not function as a part of a large complex; it exists as a dimeric protein yet independently performs critical enzymatic tasks. Its primary function is to prevent the accumulation of excessive monoamines which could lead to neurotoxicity and affect cellular homeostasis.

Pathways

MAO plays an important role in the catecholamine and serotonin signaling pathways. In particular it functions in degrading serotonin and catecholamines affecting neurotransmission balance. Through these pathways MAO interacts with other critical proteins such as catechol-O-methyltransferase (COMT) and dopamine beta-hydroxylase which also participate in the modulation of neurotransmitter activity.

MAO influences several conditions including depression and Parkinson's disease. Alterations in MAO activity can affect levels of serotonin and other neurotransmitters linked to these disorders. Additionally in the context of neurological diseases changes in MAO function can interact with proteins like alpha-synuclein potentially contributing to neurodegenerative processes associated with Parkinson's disease.
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Product protocols

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Target data

Catalyzes the oxidative deamination of primary and some secondary amine such as neurotransmitters, with concomitant reduction of oxygen to hydrogen peroxide and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues (PubMed : 18391214, PubMed : 20493079, PubMed : 24169519, PubMed : 8316221). Preferentially oxidizes serotonin (PubMed : 20493079, PubMed : 24169519). Also catalyzes the oxidative deamination of kynuramine to 3-(2-aminophenyl)-3-oxopropanal that can spontaneously condense to 4-hydroxyquinoline (By similarity).
See full target information MAOA
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica 149:21 PubMed40024917

2025

Evidence of COMT dysfunction in the olfactory bulb in Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Leah C Beauchamp,Laura J Ellett,Sydney M A Juan,Xiang M Liu,Cameron P J Hunt,Clare L Parish,Laura H Jacobson,Claire E Shepherd,Glenda M Halliday,Ashley I Bush,Laura J Vella,David I Finkelstein,Kevin J Barnham

Frontiers in bioengineering and biotechnology 12:1420183 PubMed39175618

2024

Assessment of CRISPRa-mediated overexpression in an Parkinson's disease model.

Applications

Unspecified application

Species

Unspecified reactive species

Paula Guzmán-Sastoque,Sebastián Sotelo,Natalia P Esmeral,Sonia Luz Albarracín,Jhon-Jairo Sutachan,Luis H Reyes,Carolina Muñoz-Camargo,Juan C Cruz,Natasha I Bloch

Molecules (Basel, Switzerland) 29: PubMed38893322

2024

Identification and Evaluation of Olive Phenolics in the Context of Amine Oxidase Enzyme Inhibition and Depression: In Silico Modelling and In Vitro Validation.

Applications

Unspecified application

Species

Unspecified reactive species

Tom C Karagiannis,Katherine Ververis,Julia J Liang,Eleni Pitsillou,Siyao Liu,Sarah M Bresnehan,Vivian Xu,Stevano J Wijoyo,Xiaofei Duan,Ken Ng,Andrew Hung,Erik Goebel,Assam El-Osta

Animals : an open access journal from MDPI 13: PubMed37627369

2023

Effects of Diets Based on Hydrolyzed Chicken Liver and Different Protein Concentrations on the Formation and Deamination of Biogenic Amines and Total Antioxidant Capacity of Dogs.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline Fredrich Dourado Pinto,Camila Figueiredo Carneiro Monteiro,Marcelino Bortolo,Fábio Ritter Marx,Jorge Felipe Argenta Model,Anapaula Sommer Vinagre,Luciano Trevizan

Acta pharmacologica Sinica 44:1948-1961 PubMed37225849

2023

Loss of DJ-1 function contributes to Parkinson's disease pathogenesis in mice via RACK1-mediated PKC activation and MAO-B upregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Le-le Liu,Yu Han,Zi-Jia Zhang,Yi-Qi Wang,Yu-Wei Hu,Elena Kaznacheyeva,Jian-Qing Ding,Dong-Kai Guo,Guang-Hui Wang,Bin Li,Hai-Gang Ren

International journal of molecular sciences 24: PubMed37240373

2023

Parkin Precipitates on Mitochondria via Aggregation and Autoubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Mustafa T Ardah,Nada Radwan,Engila Khan,Tohru Kitada,M Emdadul Haque
View all publications
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