MTS Assay Kit (Cell Proliferation) (Colorimetric)

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Biochemical assay - MTS Assay Kit (Cell Proliferation) (Colorimetric) (AB197010)

Diagram showing the principles of the MTS assay method.

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PubMed

Functional Studies - MTS Assay Kit (Cell Proliferation) (Colorimetric) (AB197010)

Fibroblast Cell Viability MTS Assay.

Leipnitz G et al. used MTS assay kit ab197010 in their study of mitochondrial complex I deficiency.

They used the MTS assay to examine the reduction in the cell viability of ND6 deficient and ACAD9 deficient fibroblasts, compared to wild type, when cultured in the absence and presence of glucose.

Leipnitz G et al. Sci Rep. 2018; 8: 1165. doi: 10.1038/s41598-018-19543-3 Reproduced under the creative commons license http://creativecommons.org/licenses/by/4.0/.

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Functional Studies - MTS Assay Kit (Cell Proliferation) (Colorimetric) (AB197010)

Absorbance at OD=490 nm is proportional to cell density.

Jurkat cells were cultured at different densities overnight at 37°C in a final volume of 200 μL/well. MTS reagent was added and absorbance at OD=490 nm was recorded using ELISA plate reader. Each point represents a mean of 3 replicates. Assay was performed according to the kit protocol.

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PubMed

Functional Studies - MTS Assay Kit (Cell Proliferation) (Colorimetric) (AB197010)

Hepatocyte Cell Viability studied with MTS assay.

Cabrera et al. used MTS assay kit ab197010 as part of studying ANDRO (Andrographolide), an anti-inflammatory compound with potential for use in the treatment of nonalcoholic steatohepatitis.

They used the MTS assay to demonstrate that ANDRO did not affect cell viability at the concentrations used in their study.

Cabrera D et al. Sci Rep. 2017; 7: 3491. doi: 10.1038/s41598-017-03675-z. Reproduced under the creative commons license http://creativecommons.org/licenses/by/4.0/.

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PubMed

Functional Studies - MTS Assay Kit (Cell Proliferation) (Colorimetric) (AB197010)

Viability of 4T1 cells using MTS assay.

Amirshaghaghi A et al. used MTS assay kit ab197010 in their study of a new preparation of the Chlorin e6 photosensitizer (Ce6). Ce6 is used in photodynamic tumor therapy : photosensitizers localized to tumor cells produce cytotoxic reactive oxygen species on exposure to specific wavelengths of light.

They used the MTS assay kit to measure the viability of 4T1 cells treated with different concentrations of their Ce6 preparation, with and without laser irradiation (665 nm, 5 J/cm²).

Amirshaghaghi A et al. Sci Rep. 2019; 9: 2613. doi: 10.1038/s41598-019-39036-1. Reproduced under the creative commons license http://creativecommons.org/licenses/by/4.0/.