MTT Assay Kit ab211091 is an easy-to-use, non-radioactive, and high-throughput assay for measuring cell proliferation, cell viability and cytotoxicity.
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- Communications biology 7:4862024A viral assembly inhibitor blocks SARS-CoV-2 replication in airway epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Du,Fred Deiter,Mohamed S Bouzidi,Jean-Noël Billaud,Graham Simmons,Prerna Dabral,Suganya Selvarajah,Anuradha F Lingappa,Maya Michon,Shao Feng Yu,Kumar Paulvannan,Balaji Manicassamy,Vishwanath R Lingappa,Homer Boushey,John R Greenland,Satish K PillaiPubMed 38649430
- Chemical biology & drug design 103:e145192024Kaempferol attenuates inflammation in lipopolysaccharide-induced gallbladder epithelial cells by inhibiting the MAPK/NF-κB signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHai-Tao Wu,Xin-Xing Lin,Xiao-Lei Yang,Yong Ding,Jia-Liang Wang,Chen-Lu Liu,Wei-Zhou YuPubMed 38570708
- Haematologica 109:725-7392024Aberrant MNX1 expression associated with t(7;12)(q36;p13) pediatric acute myeloid leukemia induces the disease through altering histone methylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAhmed Waraky,Anders Östlund,Tina Nilsson,Dieter Weichenhan,Pavlo Lutsik,Marion Bähr,Joschka Hey,Gürcan Tunali,Jenni Adamsson,Susanna Jacobsson,Mohammad Hamdy Abdelrazak Morsy,Susann Li,Linda Fogelstrand,Christoph Plass,Lars PalmqvistPubMed 37317878
- ImmunoHorizons 8:198-2132024Erdr1 Drives Macrophage Programming via Dynamic Interplay with YAP1 and Mid1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuhang WangPubMed 38392560
- Nature communications 15:5412024Apoptosis-mediated ADAM10 activation removes a mucin barrier promoting T cell efferocytosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinnea Z Drexhage,Shengpan Zhang,Maeva Dupont,Franziska Ragaller,Ellen Sjule,Jose Cabezas-Caballero,Lachlan P Deimel,Helen Robertson,Rebecca A Russell,Omer Dushek,Erdinc Sezgin,Niloofar Karaji,Quentin J SattentauPubMed 38225245
- JHEP reports : innovation in hepatology 6:1009592024Adenosine A2a receptor inhibition increases the anti-tumor efficacy of anti-PD1 treatment in murine hepatobiliary cancers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuta Myojin,Justin D McCallen,Chi Ma,Kylynda C Bauer,Benjamin Ruf,Mohamed-Reda Benmebarek,Benjamin L Green,Simon Wabitsch,John C McVey,Claude Fu,Changqing Xie,Tim F GretenPubMed 38192537
- Nucleic acids research 52:1173-11872023The protein phosphatase EYA4 promotes homologous recombination (HR) through dephosphorylation of tyrosine 315 on RAD51.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBárbara de la Peña Avalos,Nicolas Paquet,Romain Tropée,Yan Coulombe,Hannah Palacios,Justin W Leung,Jean-Yves Masson,Pascal H G Duijf,Eloïse DrayPubMed 38084915
- Aging 15:13646-136542023Cabozantinib prevents AGEs-induced degradation of type 2 collagen and aggrecan in human chondrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Dong,Lianfang Lin,Yuan Ji,Xu Cheng,Zhiwu ZhangPubMed 38059882
- International journal of molecular sciences 24:2023A New Bioactive Fibrin Formulation Provided Superior Cartilage Regeneration in a Caprine Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElif Vardar,Hui Yin Nam,Ganesh Vythilingam,Han Ling Tan,Haryanti Azura Mohamad Wali,Eva-Maria Engelhardt,Tunku Kamarul,Pierre-Yves Zambelli,Eleftheria SamaraPubMed 38069268
- Frontiers in immunology 14:12121672023Hepatocyte-specific regulation of autophagy and inflammasome activation via MyD88 during lethal infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOmid Teymournejad,Aditya Kumar Sharma,Mohammed Abdelwahed,Muhamuda Kader,Ibrahim Ahmed,Hoda Elkafas,Nahed IsmailPubMed 38022511
Key facts
- Detection method
- Colorimetric
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Quantitative
- Reactive species
- Mammals
- Assay time
- 3h 15m
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MTT Assay Kit ab211091 is an easy-to-use, non-radioactive, and high-throughput assay for measuring cell proliferation, cell viability and cytotoxicity.
Key facts
- Detection method
- Colorimetric
- Sample types
- Suspension cells, Adherent cells
- Assay type
- Quantitative
- Reactive species
- Mammals
- Assay time
- 3h 15m
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
MTT Assay Kit ab211091 is an easy-to-use, non-radioactive, and high-throughput assay for measuring cell proliferation, cell viability and cytotoxicity.
How the assay works
The MTT assay protocol is based on the conversion of water soluble MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) compound to an insoluble formazan product. NB: MTT is also available as free molecule as Thiazolyl blue tetrazolium bromide (MTT), membrane-permeable dye ab146345 (Thiazolyl blue tetrazolium bromide).
Viable cells with active metabolism convert MTT into formazan. Dead cells lose this ability and therefore show no signal. Thus color formation serves as a useful and convenient marker of only the viable cells. The measured absorbance at OD 590 nm is proportional to the number of viable cells.
The MTT assay is a measure of the metabolic activity of the cells analyzed; the more metabolic activity in the sample, the higher the signal. This should be considered when interpreting the results of the assay.
MTT assay protocol summary
- - Replace serum-containing media with serum-free media and MTT reagent in cell cultures
- - Incubate for 3 hr at 37°C
- - Add MTT solvent and incubate for 15 min
- - Analyze with microplate reader
Related cell proliferation assay products
MTS assay kit MTS Assay Kit (Cell Proliferation) (Colorimetric) ab197010, uses a similar principle to this kit, but without the need for the MTT solvent step.
How other researchers are using MTT Assay Kit ab211091
This MTT assay kit has been used in publications with a variety of cell types, including: HUVEC cells1, U2OS cells and Saos2 cells2, human PBMCs3, rat primary hepatic stellate cells4, DBTRG glioblastoma cells5, SKOV3 human ovarian cancer cells6, mouse vascular smooth muscle cells7, human iPSC-derived neuronal cultures8, primary bladder cancer cells9
References: 1-De Felice F et al 2019, 2-Zhang X et al 2019, 3-Schotterl S et al 2018, 4-Huang W et al 2015, 5-Tang XJ et al 2016, 6-Zhu Q et al 2017, 7-Ma S et al 2018, 8-Rane A et al 2018, 9-Maj M et al 2018
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
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3 product images
Image courtesy of an anonymous customer reviewFunctional Studies - MTT Assay Kit (Cell Proliferation) (ab211091)
MTT assay of Bortezomib in multiple myeloma cells.
MTT assay kit ab211091 was used by an Abcam customer (see product review) to assess the cell viability of myeloma cells which have acquired resistance to bortezomib (a proteasome inhibitor used as a cancer therapeutic).
Cells previously treated with 10 nM bortezomib for 1.5 months (RPMI8226-Bortezomib) had significantly improved viability, compared to the parental untreated cell line, when treated for 72 hours with varying concentrations of bortezomib.
Functional Studies - MTT Assay Kit (Cell Proliferation) (ab211091)
HeLa cells were grown in DMEM media supplemented with 10% FBS, harvested using trypsin and counted using Trypan blue and a hemocytometer. Cells were then serially diluted in a clear cell culture plate and incubated for 3 hours with MTT Reagent at 37°C. After incubation, cells were treated with MTT Solvent for 15 minutes at room temperature. Absorbance was measured at OD = 590 nm. Inset graph is an expanded segment of the assay data at lower cell number per well.
Biochemical assay - MTT Assay Kit (Cell Proliferation) (ab211091)
Diagram showing the principles of the MTT assay method.
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