P-glycoprotein (MDR1) is a member of the ATP-binding cassette (ABC) ATPase superfamily of transmembrane transporter proteins. P-glycoprotein has an extremely broad substrate specificity and is capable of transporting a vast array of neutral and anionic lipophilic molecules.

P-glycoprotein Ligand Screening Kit (ab284553) (K507) is designed for rapidly screening test compounds for modulation of efflux transporter activity in MDR1-expressing cell lines. The assay uses a lipophilic non-fluorescent P-glycoprotein substrate that readily diffuses through the plasma membrane, where it is hydrolyzed to an active fluorophore by cytosolic esterases. The resulting hydrophilic fluorophore is neither membrane permeable nor a substrate for P-glycoprotein, hence it remains trapped inside the cell. The assay is highly sensitive, has a simple no-wash protocol and is high throughput adaptable. The kit contains a complete set of reagents sufficient for performing 100 reactions in a 96-well plate format.

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Multidrug Efflux Transporter P Glycoprotein (MDR1/P-gp) Ligand Screening Kit (ab284553) is unavailable

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