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AB289834

Mycoplasma PCR Detection Kit

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(13 Publications)

Mycoplasma PCR Detection Kit ab289834 helps to minimize false positives while ensuring coverage of over 200 species/strains of mycoplasmas with a quick protocol for routine screening.

- Results in under 2 hrs
- No DNA extraction required; add cell culture media sample directly to PCR reaction
- detect 200+ Mycoplasma species
- Includes ready-to-use primer mix and positive control
1 Images
Electrophoresis - Mycoplasma PCR Detection Kit (AB289834)
  • Electrophoresis

Lab

Electrophoresis - Mycoplasma PCR Detection Kit (AB289834)

Mycoplasma PCR Detection Kit ab289834 offers rapid and reliable screening for mycoplasma contamination in cell cultures. This gel image shows the assay results, where the positive control (+) confirms successful amplification with a 500 bp band, and samples 2-4 test positive for Mycoplasma with bands in the expected 370-550 bp range; samples 1 and 5 show no contamination. The no-template control (NTC) is clean, validating the specificity of the assay - no DNA isolation required.

Key facts

Sample types

Cell culture media

Results type

Qualitative

Product details

Mycoplasma PCR Detection Kit ab289834 offers rapid and reliable screening for mycoplasma contamination in cell cultures. It gives highly specific and sensitive detection of 200+ strains of Mycoplasma in less than 2 hours with a quick protocol for routine screening. In addition to the short detection process (less than 2 hours), the easy handling and high sensitivity makes this kit a convenient tool for routine examination of cell cultures and media.

Mycoplasma PCR Detection Kit protocol summary
- Prepare the PCR reagents and premix solution
- Add the sample to the PCR reaction mix.
- Perform the PCR cycling

Analyze the PCR products using agarose gel electrophoresis. A band at ~500bp indicates mycoplasma contamination.

What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mycoplasma are a group of bacteria without a cell wall which makes them unique and adaptable to various environments. Known for their small size they have a low genomic mass. They parasitize the cells of a host organism relying on host cells for nutrients and energy. Mycoplasma can express themselves in human animal and plant hosts often found in cultures used in research laboratories. Due to their lack of cell walls they can take on various shapes which complicates their detection and monitoring in lab settings.
Biological function summary

The infection caused by these bacteria can disrupt normal cellular processes of the host. Their evasion of the immune system occurs through frequent genetic changes leading to persistent infections. Mycoplasma are not part of a complex; however they interact closely with cellular structures of the host leading researchers to employ techniques like 'mycoplasma detection kit' or 'mycoplasma PCR test' to identify contamination. These methods are necessary due to the bacteria's ability to impact cellular morphology and function.

Pathways

Various processes are disrupted upon infection by these bacteria affecting essential pathways such as DNA replication and signal transduction. For example they interfere with host cell functions through interactions with proteins like toll-like receptors which are part of the host's immune response. Mycoplasma bacteria may directly alter the metabolic flow within the pathways influencing cellular productivity and viability. The 'mycoplasma testing kit' becomes valuable in identifying these bacteria ensuring accurate experimental outcomes.

Mycoplasma bacteria are associated with respiratory infections and urogenital tract disorders. In humans Mycoplasma pneumoniae causes atypical pneumonia while Mycoplasma genitalium is linked to urogenital tract infections. Their presence in cell cultures necessitates the use of tools such as the 'mycoplasma PCR kit' to prevent compromised research. These bacteria are connected to disease processes through interactions with proteins involved in the host immune response posing challenges in treatment and necessitating continued scientific investigation.

Product protocols

Target data

Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:5547 PubMed40593553

2025

Host glutathione is required for Rickettsia parkeri cell division and intracellular survival.

Applications

Unspecified application

Species

Unspecified reactive species

Han Sun,Anh Phuong Luu,Meggie Danielson,Thomas P Burke

Research (Washington, D.C.) 8:0703 PubMed40416363

2025

Targeting the Exonic Circular RNA/O-GlcNAc Transferase/Forkhead Box C1 Axis Inhibits Asparagine- and Alanine-Mediated Ferroptosis Repression in Neuroblastoma Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Qilan Li,Yang Cheng,Chunhui Yang,Minxiu Tian,Xiaojing Wang,Dan Li,Xinyue Li,Jiaying Qu,Shunchen Zhou,Liduan Zheng,Qiangsong Tong

iScience 27:111385 PubMed39669426

2024

Cytosolic bacterial pathogens activate TLR pathways in tumors that synergistically enhance STING agonist cancer therapies.

Applications

Unspecified application

Species

Unspecified reactive species

Meggie Danielson,Christopher J Nicolai,Thaomy T Vo,Natalie K Wolf,Thomas P Burke

Journal for immunotherapy of cancer 12: PubMed39631847

2024

Natural lung-tropic T9 cells: a sharp weapon for established lung metastases.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Chen,Chenxiao Qiao,Eloy Yinwang,Shengdong Wang,Xuehuan Wen,Yixuan Feng,Xiangang Jin,Shuming Li,Yucheng Xue,Hao Zhou,Wenkan Zhang,Xianchang Zeng,Zenan Wang,Hangxiang Sun,Lifeng Jiang,Hengyuan Li,Binghao Li,Zhijian Cai,Zhaoming Ye,Nong Lin

Frontiers in cellular and infection microbiology 14:1497427 PubMed39624268

2024

KIF20A activated by transcription factor GATA2 promotes cell growth in hepatitis B virus-related hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Xu,Wenhua Cheng,Yi Wang,Yunpeng Zhou,Zhiming Wang,Yunyan Dai,Yaoxuan Li,Pinggui Chen,Ting Liu,Yifan Li,Gaopeng Li,Wenqing Qu,Jing Chen

British journal of cancer 131:589-600 PubMed38886556

2024

Aberrant activation of AXL may drive progression of squamous cell carcinoma in CLL patients: a mechanistic study with clinical implications.

Applications

Unspecified application

Species

Unspecified reactive species

Sutapa Sinha,Ray Guo,Miles D Del Busso,Weiguo Han,Justin Boysen,Linda E Wellik,Asish K Ghosh,Neil E Kay

Epigenetics & chromatin 17:3 PubMed38336688

2024

A low-input high resolution sequential chromatin immunoprecipitation method captures genome-wide dynamics of bivalent chromatin.

Applications

Unspecified application

Species

Unspecified reactive species

Janith A Seneviratne,William W H Ho,Eleanor Glancy,Melanie A Eckersley-Maslin

Toxics 11: PubMed38133399

2023

Perturbations in Osteogenic Cell Fate Following Exposure to Constituents Present in Tobacco: A Combinatorial Study.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph V Madrid,Madeline K M Vera-Colón,Nicole I Zur Nieden

Oncogenesis 12:53 PubMed37949862

2023

Coordinate transcriptional regulation of ErbB2/3 by C-terminal binding protein 2 signals sensitivity to ErbB2 inhibition in pancreatic adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kranthi Kumar Chougoni,Haemin Park,Priyadarshan K Damle,Travis Mason,Bo Cheng,Martin M Dcona,Barbara Szomju,Mikhail G Dozmorov,Michael O Idowu,Steven R Grossman

Cancers 15: PubMed37835514

2023

Taxifolin Inhibits the Growth of Non-Small-Cell Lung Cancer via Downregulating Genes Displaying Novel and Robust Associations with Immune Evasion Factors.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaozeng Lin,Ying Dong,Yan Gu,Fengxiang Wei,Jingyi Peng,Yingying Su,Yanjun Wang,Chengzhi Yang,Sandra Vega Neira,Anil Kapoor,Damu Tang
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