Mycoplasma PCR Detection Kit
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(13 Publications)
- Results in under 2 hrs
- No DNA extraction required; add cell culture media sample directly to PCR reaction
- detect 200+ Mycoplasma species
- Includes ready-to-use primer mix and positive control
- Electrophoresis
Lab
Electrophoresis - Mycoplasma PCR Detection Kit (AB289834)
Mycoplasma PCR Detection Kit ab289834 offers rapid and reliable screening for mycoplasma contamination in cell cultures. This gel image shows the assay results, where the positive control (+) confirms successful amplification with a 500 bp band, and samples 2-4 test positive for Mycoplasma with bands in the expected 370-550 bp range; samples 1 and 5 show no contamination. The no-template control (NTC) is clean, validating the specificity of the assay - no DNA isolation required.
Product details
Mycoplasma PCR Detection Kit ab289834 offers rapid and reliable screening for mycoplasma contamination in cell cultures. It gives highly specific and sensitive detection of 200+ strains of Mycoplasma in less than 2 hours with a quick protocol for routine screening. In addition to the short detection process (less than 2 hours), the easy handling and high sensitivity makes this kit a convenient tool for routine examination of cell cultures and media.
Mycoplasma PCR Detection Kit protocol summary
- Prepare the PCR reagents and premix solution
- Add the sample to the PCR reaction mix.
- Perform the PCR cycling
Analyze the PCR products using agarose gel electrophoresis. A band at ~500bp indicates mycoplasma contamination.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The infection caused by these bacteria can disrupt normal cellular processes of the host. Their evasion of the immune system occurs through frequent genetic changes leading to persistent infections. Mycoplasma are not part of a complex; however they interact closely with cellular structures of the host leading researchers to employ techniques like 'mycoplasma detection kit' or 'mycoplasma PCR test' to identify contamination. These methods are necessary due to the bacteria's ability to impact cellular morphology and function.
Pathways
Various processes are disrupted upon infection by these bacteria affecting essential pathways such as DNA replication and signal transduction. For example they interfere with host cell functions through interactions with proteins like toll-like receptors which are part of the host's immune response. Mycoplasma bacteria may directly alter the metabolic flow within the pathways influencing cellular productivity and viability. The 'mycoplasma testing kit' becomes valuable in identifying these bacteria ensuring accurate experimental outcomes.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (13)
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Nature communications 16:5547 PubMed40593553
2025
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Research (Washington, D.C.) 8:0703 PubMed40416363
2025
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iScience 27:111385 PubMed39669426
2024
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Journal for immunotherapy of cancer 12: PubMed39631847
2024
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Frontiers in cellular and infection microbiology 14:1497427 PubMed39624268
2024
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British journal of cancer 131:589-600 PubMed38886556
2024
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Epigenetics & chromatin 17:3 PubMed38336688
2024
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Toxics 11: PubMed38133399
2023
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Oncogenesis 12:53 PubMed37949862
2023
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Cancers 15: PubMed37835514
2023
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com