Myeloperoxidase (MPO) Activity Assay Kit ab105136 is a rapid, simple, sensitive, and reliable colorimetric assay suitable for use as a high throughput MPO activity assay. Readout is on any colorimetric (412 nm) plate reader.
- Cited in > 90 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- Bioactive materials 34:138-1492024Oral TNF-α siRNA delivery via milk-derived exosomes for effective treatment of inflammatory bowel disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGeonhee Han,Hyosuk Kim,Hochung Jang,Eun Sun Kim,Sun Hwa Kim,Yoosoo YangPubMed 38223538
- Investigative ophthalmology & visual science 64:12023Interleukin-11 Suppresses Ocular Surface Inflammation and Accelerates Wound Healing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAastha Singh,WonKyung J Cho,Vinay K Pulimamidi,Sharad K Mittal,Sunil K ChauhanPubMed 37910094
- Animals : an open access journal from MDPI 13:2023Melatonin Alleviates Lipopolysaccharide-Induced Endometritis by Inhibiting the Activation of NLRP3 Inflammasome through Autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYujin Gao,Yina Li,Jiamian Wang,Xijun Zhang,Dan Yao,Xuanpan Ding,Xingxu Zhao,Yong ZhangPubMed 37570258
- PloS one 18:e02888132023Rosmarinic acid ameliorates HCl-induced cystitis in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNaoya Kitamura,Yasunori Yamamoto,Naoki Yamamoto,Takatoshi MurasePubMed 37463180
- Cells 12:2023Sex-Based Disparities in Leukocyte Migration and Activation in Response to Inhalation Lung Injury: Role of SDF-1/CXCR4 Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTanima Chatterjee,Terry L Lewis,Itika Arora,Anastasiia E Gryshyna,Lilly Underwood,Juan Xavier Masjoan Juncos,Saurabh AggarwalPubMed 37443753
- Communications medicine 3:622023Myeloperoxidase inhibition may protect against endothelial glycocalyx shedding induced by COVID-19 plasma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrew Teo,Louisa L Y Chan,Christine Cheung,Po Ying Chia,Sean Wei Xiang Ong,Siew Wai Fong,Lisa F P Ng,Laurent Renia,David Chien Lye,Barnaby Edward Young,Tsin Wen YeoPubMed 37147421
- International journal of molecular sciences 24:2023Essential Involvement of Neutrophil Elastase in Acute Acetaminophen Hepatotoxicity Using BALB/c Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuko Ishida,Siying Zhang,Yumi Kuninaka,Akiko Ishigami,Mizuho Nosaka,Isui Harie,Akihiko Kimura,Naofumi Mukaida,Toshikazu KondoPubMed 37175553
- Journal of inflammation (London, England) 20:132023Efficacy of CU06-1004 via regulation of inflammation and endothelial permeability in LPS-induced acute lung injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYeomyeong Kim,Cho-Rong Bae,Dongyeop Kim,Hyejeong Kim,Sunghye Lee,Haiying Zhang,Minyoung Noh,Young-Myeong Kim,Naoki Mochizuki,Young-Guen KwonPubMed 37024954
- Journal of cardiothoracic surgery 18:922023Protective role of FBXL19 in Streptococcus pneumoniae-induced lung injury in pneumonia immature mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiqiang Chen,Bijuan Zheng,Zhiwei Zhang,Zhiyong HuangPubMed 36964598
- International journal of molecular sciences 24:2023Uridine Alleviates Sepsis-Induced Acute Lung Injury by Inhibiting Ferroptosis of Macrophage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai Lai,Congkuan Song,Minglang Gao,Yu Deng,Zilong Lu,Ning Li,Qing GengPubMed 36982166
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Homogenate, Cell culture supernatant, Serum, Other biological fluids, Urine (UTI)
- Assay type
- Enzyme activity
- Reactive species
- Mammals
- Assay time
- 1h
Associated Products
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2 products for Alternative Product
Target data
Function
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed:9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed:25698971).
Alternative names
Myeloperoxidase, MPO
What's included?
Recommended products
Myeloperoxidase (MPO) Activity Assay Kit ab105136 is a rapid, simple, sensitive, and reliable colorimetric assay suitable for use as a high throughput MPO activity assay. Readout is on any colorimetric (412 nm) plate reader.
- Cited in > 90 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Homogenate, Cell culture supernatant, Serum, Other biological fluids, Urine (UTI)
- Assay type
- Enzyme activity
- Reactive species
- Mammals
- Assay time
- 1h
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
How the assay works
In the Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) protocol, myeloperoxidase produces HClO from H2O2 and Cl-. The HClO reacts with taurine to generate the taurine chloramine, which subsequently reacts with the DTNB probe to eliminate color (absorbance at 412 nm). The absorbance is inversely proportional to the amount of MPO enzyme.
Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) protocol summary:
- Add samples to wells (and assay buffer only to wells for standards)
- Add reaction mix to sample wells and incubate for 30 min to 2 hrs
- Add stop mix and incubate for 10 min
- Add TNB reagent/standard to all wells and incubate for 5-10 min
- Analyze with a microplate reader
Technical Note: MPO is unlikely to be detectable in healthy urine but may be elevated in the presence of infection.
How other researchers are using
Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) has been used in a variety of sample type including:
HEK293T cells 1
Mice corneal lysates 2
Mouse uterus samples 3
References:
1-Han G et al. 2023
2-Singh A et al. 2023
3-Gao Y J et al. 2023
Related and recommended products
For fluorometric detection, we recommend the following kits:
- Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) ab111749
- Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric - Red) Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric - Red) ab219925
- Myeloperoxidase (MPO) Peroxidation Activity Assay Kit (Fluorometric) Myeloperoxidase (MPO) Peroxidation Activity Assay Kit (Fluorometric) ab273334
This product is manufactured by BioVision, an Abcam company and was previously called K744 Myeloperoxidase (MPO) Colorimetric Activity Assay Kit. K744-100 is the same size as the 100 test size of ab105136.
Myeloperoxidase (MPO) is a peroxidase enzyme (EC 1.11.1.7) most abundantly expressed in neutrophil granulocytes. It is a lysosomal protein stored in the azurophilic granules of the neutrophil. Myeloperoxidase contains a heme pigment which causes its green color in secretions rich in neutrophils, such as pus and some forms of mucus. Myeloperoxidase catalyzes the production of hypochlorous acid (HClO) from hydrogen peroxide (H2O2) and chloride anion (Cl-, or the equivalent from a non-chlorine halide). Myeloperoxidase also oxidizes tyrosine to a tyrosyl radical using hydrogen peroxide as an oxidizing agent.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.
- Biological function summary
The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.
- Pathways
Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.
- Associated diseases and disorders
Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.
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3 product images
Functional Studies - Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) (ab105136)
a. Measurement of MPO activity using WBC lysate (3 μg) and MPO Positive Control (5 and 10 μL). b. MPO specific activity in WBC lysate.
Namkoong, Ho et al., PLoS pathogens: vol. 14,4 e1006955., S4 fig, doi:10.1371/journal.ppat.1006955Functional Studies - Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) (ab105136)
MPO activity in indicated cells was measured using the commercially available MPO Activity Assay Kit (n = 3).
Functional Studies - Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) (ab105136)
Typical standard curve for ab105136.
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