AB111749

Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric)

Name: Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) (ab111749) | Abcam
Brand: Abcam Limited
SKU: AB111749
Price: 745 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(18 Publications)

Myeloperoxidase (MPO) Activity Assay Kit ab111749 provides a rapid, simple and reliable fluorometric assay to study MPO activity.

View Alternative Names

Myeloperoxidase, MPO

2 Images

FuncS

Supplier Data

Functional Studies - Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) (AB111749)

Standard Curve using this kit protocol (Ex/Em = 485/525 nm)

FuncS

Supplier Data

Functional Studies - Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) (AB111749)

a. Measurement of MPO activity in WBC lysate (0.1 μg) and MPO Positive Control (3 μL). b. MPO specific activity in WBC lysate.

Key facts

Detection method

Fluorescent

Sample types

Tissue Extracts, Cell culture supernatant

Assay type

Enzyme activity

Sensitivity

> 0.0005 mU/well

Assay time

30m

Assay Platform

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Myeloperoxidase (MPO) Activity Assay Kit ab111749 provides a rapid, simple and reliable fluorometric assay to study MPO activity.

In the MPO assay protocol, MPO catalyzes the production of sodium hypochlorite (NaClO) from hydrogen peroxide (H₂O₂) and sodium chloride (NaCl). Subsequently, NaClO reacts stoichiometrically with the free radical sensor Aminophenyl fluorescein (APF) to generate fluorescein, whose fluorescence can be detected at Ex/Em = 485/525 nm.

This MPO assay kit can be used to detect MPO activity as low as 0.5 μU per well.

MPO assay protocol summary:
- add fluoroscein standard to wells and analyze with a microplate reader
- add samples to wells
- add reaction mix
- analyze every 2-3 mins with a microplate reader in kinetic mode for at least 30 mins

This product is manufactured by BioVision, an Abcam company and was previously called K745 Myeloperoxidase (MPO) Fluorometric Activity Assay Kit. K745-100 is the same size as the 100 test size of ab111749.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab111749-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"MPO Positive Control", "number":"AB111749-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 54", "number":"AB111749-CMP01", "productcode":"" }, { "size":"1 x 50 µL", "name":"Hydrogen Peroxide Solution", "number":"AB111749-CMP05", "productcode":"" }, { "size":"1 x 50 µL", "name":"Fluorescence Standard IV", "number":"AB111749-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"MPO Chlorination Probe", "number":"AB111749-CMP04", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.

Biological function summary

The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.

Pathways

Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.

Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.

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Product protocols

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Target data

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed : 9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed : 25698971).

See full target information MPO

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Toxics 11: PubMed36668787

2023

Can Maternal Exposure to Air Pollution Affect Post-Natal Liver Development?

Applications

Unspecified application

Species

Unspecified reactive species

Yong Song,Ling Chen,Ellen Bennett,Amanda J Wheeler,Katherine Southam,Seiha Yen,Fay Johnston,Graeme R Zosky

Stem cell research & therapy 13:479 PubMed36153571

2022

TNFAIP6 defines the MSC subpopulation with enhanced immune suppression activities.

Applications

Unspecified application

Species

Unspecified reactive species

Lingyun Li,Lei Yang,Xian Chen,Xiangjuan Chen,Lianghui Diao,Yong Zeng,Jianyong Xu

Journal of healthcare engineering 2022:8450673 PubMed35399858

2022

Gu-Ben-Zhi-Ke-Zhong-Yao Alleviated PM2.5-Induced Lung Injury via HMGB1/NF-B Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenxiao Hou,Hongchun Zhang,Meng Jiang,Yina Wu,Tao Li,Luhong Cong,Jun Duan

The Journal of biological chemistry 298:101847 PubMed35314195

2022

Capsaicin inhibits intestinal Cl secretion and promotes Na absorption by blocking TRPV4 channels in healthy and colitic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hanxing Wan,Xiong Ying Chen,Fenglian Zhang,Jun Chen,Fenglan Chu,Zachary M Sellers,Feng Xu,Hui Dong

mSphere 7:e0084721 PubMed35044805

2022

Nontypeable Haemophilus influenzae Redox Recycling of Protein Thiols Promotes Resistance to Oxidative Killing and Bacterial Survival in Biofilms in a Smoke-Related Infection Model.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin C Hunt,Xin Xu,Amit Gaggar,W Edward Swords

Nutrients 13: PubMed33920187

2021

Whole and Isolated Protein Fractions Differentially Affect Gastrointestinal Integrity Markers in C57Bl/6 Mice Fed Diets with a Moderate-Fat Content.

Applications

Unspecified application

Species

Unspecified reactive species

Tara R Price,Sangeetha A Baskaran,Kristin L Moncada,Yasushi Minamoto,Cory Klemashevich,Arul Jayuraman,Jan S Sucholdoski,Luis O Tedeschi,Jörg M Steiner,Suresh D Pillai,Rosemary L Walzem

American journal of physiology. Endocrinology and metabolism 320:E150-E159 PubMed33284091

2020

Myostatin deficiency not only prevents muscle wasting but also improves survival in septic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Masayuki Kobayashi,Shingo Kasamatsu,Shohei Shinozaki,Shingo Yasuhara,Masao Kaneki

Journal of innate immunity 13:83-93 PubMed33045713

2020

PolyADP-Ribosylation of NFATc3 and NF-κB Transcription Factors Modulate Macrophage Inflammatory Gene Expression in LPS-Induced Acute Lung Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yunjuan Nie,Teja Srinivas Nirujogi,Ravi Ranjan,Brenda F Reader,Sangwoon Chung,Megan N Ballinger,Joshua A Englert,John W Christman,Manjula Karpurapu

Frontiers in medicine 7:201 PubMed32582723

2020

Kidney Ischemia-Reperfusion Elicits Acute Liver Injury and Inflammatory Response.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Shang,Susara Madduma Hewage,Charith U B Wijerathne,Yaw L Siow,Cara K Isaak,Karmin O

Nature communications 10:1985 PubMed31064987

2019

Regeneration of severely damaged lungs using an interventional cross-circulation platform.

Applications

Unspecified application

Species

Unspecified reactive species

Brandon A Guenthart,John D O'Neill,Jinho Kim,Dawn Queen,Scott Chicotka,Kenmond Fung,Michael Simpson,Rachel Donocoff,Michael Salna,Charles C Marboe,Katherine Cunningham,Susan P Halligan,Holly M Wobma,Ahmed E Hozain,Alexander Romanov,Gordana Vunjak-Novakovic,Matthew Bacchetta

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric)

Functional Studies - Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) (AB111749)

Functional Studies - Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) (AB111749)

Key facts

Detection method

Sample types

Assay type

Sensitivity

Assay time

Assay Platform

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Assay Kits

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (18)

Product promise