Myeloperoxidase (MPO) Activity Assay Kit ab111749 provides a rapid, simple and reliable fluorometric assay to study MPO activity.
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- Toxics 11:2023Can Maternal Exposure to Air Pollution Affect Post-Natal Liver Development?Applications:Unspecified applicationReactive species:Unspecified reactive speciesYong Song,Ling Chen,Ellen Bennett,Amanda J Wheeler,Katherine Southam,Seiha Yen,Fay Johnston,Graeme R ZoskyPubMed 36668787
- Stem cell research & therapy 13:4792022TNFAIP6 defines the MSC subpopulation with enhanced immune suppression activities.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLingyun Li,Lei Yang,Xian Chen,Xiangjuan Chen,Lianghui Diao,Yong Zeng,Jianyong XuPubMed 36153571
- Journal of healthcare engineering 2022:84506732022Gu-Ben-Zhi-Ke-Zhong-Yao Alleviated PM2.5-Induced Lung Injury via HMGB1/NF-B Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenxiao Hou,Hongchun Zhang,Meng Jiang,Yina Wu,Tao Li,Luhong Cong,Jun DuanPubMed 35399858
- The Journal of biological chemistry 298:1018472022Capsaicin inhibits intestinal Cl secretion and promotes Na absorption by blocking TRPV4 channels in healthy and colitic mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHanxing Wan,Xiong Ying Chen,Fenglian Zhang,Jun Chen,Fenglan Chu,Zachary M Sellers,Feng Xu,Hui DongPubMed 35314195
- mSphere 7:e00847212022Nontypeable Haemophilus influenzae Redox Recycling of Protein Thiols Promotes Resistance to Oxidative Killing and Bacterial Survival in Biofilms in a Smoke-Related Infection Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBenjamin C Hunt,Xin Xu,Amit Gaggar,W Edward SwordsPubMed 35044805
- Nutrients 13:2021Whole and Isolated Protein Fractions Differentially Affect Gastrointestinal Integrity Markers in C57Bl/6 Mice Fed Diets with a Moderate-Fat Content.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTara R Price,Sangeetha A Baskaran,Kristin L Moncada,Yasushi Minamoto,Cory Klemashevich,Arul Jayuraman,Jan S Sucholdoski,Luis O Tedeschi,Jörg M Steiner,Suresh D Pillai,Rosemary L WalzemPubMed 33920187
- American journal of physiology. Endocrinology and metabolism 320:E150-E1592020Myostatin deficiency not only prevents muscle wasting but also improves survival in septic mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasayuki Kobayashi,Shingo Kasamatsu,Shohei Shinozaki,Shingo Yasuhara,Masao KanekiPubMed 33284091
- Journal of innate immunity 13:83-932020PolyADP-Ribosylation of NFATc3 and NF-κB Transcription Factors Modulate Macrophage Inflammatory Gene Expression in LPS-Induced Acute Lung Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunjuan Nie,Teja Srinivas Nirujogi,Ravi Ranjan,Brenda F Reader,Sangwoon Chung,Megan N Ballinger,Joshua A Englert,John W Christman,Manjula KarpurapuPubMed 33045713
- Frontiers in medicine 7:2012020Kidney Ischemia-Reperfusion Elicits Acute Liver Injury and Inflammatory Response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYue Shang et. al.PubMed 32582723
- Nature communications 10:19852019Regeneration of severely damaged lungs using an interventional cross-circulation platform.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrandon A Guenthart et. al.PubMed 31064987
Key facts
- Detection method
- Fluorescent
- Sample types
- Tissue Extracts, Cell culture supernatant
- Assay type
- Enzyme activity
- Reactive species
- Mammals
- Assay time
- 30m
- Sensitivity
- > 0.0005 mU/well
Associated Products
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2 products for Alternative Product
Target data
Function
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed:9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed:25698971).
Alternative names
Myeloperoxidase, MPO
What's included?
Recommended products
Myeloperoxidase (MPO) Activity Assay Kit ab111749 provides a rapid, simple and reliable fluorometric assay to study MPO activity.
Key facts
- Detection method
- Fluorescent
- Sample types
- Tissue Extracts, Cell culture supernatant
- Assay type
- Enzyme activity
- Reactive species
- Mammals
- Assay time
- 30m
- Assay Platform
- Microplate reader
- Sensitivity
- > 0.0005 mU/well
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Myeloperoxidase (MPO) Activity Assay Kit ab111749 provides a rapid, simple and reliable fluorometric assay to study MPO activity.
In the MPO assay protocol, MPO catalyzes the production of sodium hypochlorite (NaClO) from hydrogen peroxide (H2O2) and sodium chloride (NaCl). Subsequently, NaClO reacts stoichiometrically with the free radical sensor Aminophenyl fluorescein (APF) to generate fluorescein, whose fluorescence can be detected at Ex/Em = 485/525 nm.
This MPO assay kit can be used to detect MPO activity as low as 0.5 μU per well.
MPO assay protocol summary:
- add fluoroscein standard to wells and analyze with a microplate reader
- add samples to wells
- add reaction mix
- analyze every 2-3 mins with a microplate reader in kinetic mode for at least 30 mins
This product is manufactured by BioVision, an Abcam company and was previously called K745 Myeloperoxidase (MPO) Fluorometric Activity Assay Kit. K745-100 is the same size as the 100 test size of ab111749.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.
- Biological function summary
The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.
- Pathways
Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.
- Associated diseases and disorders
Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.
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2 product images
Functional Studies - Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) (ab111749)
a. Measurement of MPO activity in WBC lysate (0.1 μg) and MPO Positive Control (3 μL). b. MPO specific activity in WBC lysate.
Functional Studies - Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) (ab111749)
Standard Curve using this kit protocol (Ex/Em = 485/525 nm)
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