Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric - Red)

Product details

Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) (ab219925) provides a simple method to measure Myeloperoxidase (MPO) activity in cell lysates. The assay uses our AbRed Indicator to monitor myeloperoxidase (MPO) activity, leading to an increase in fluorescence that can be easily detected at an optimal Ex/Em = 540/590 nm (range: 530-570/590-600 nm). The signal can also be read by absorbance at OD: 576 ± 5 nm, although the sensitivity of the assay is reduced 10-fold.

This assay can detect activity from as low as 0.1 mU/mL Myeloperoxidase (MPO) in solution. The assay can be performed in a convenient 96-well or 384-well plate format and is easily adapted to automation without a separation or wash step.

Myeloperoxidase (MPO), most abundantly present in neutrophils and monocytes, is a green hemoprotein having peroxidase activity. It catalyzes the reaction of hydrogen peroxide and halide ions to form cytotoxic acids and other intermediates; and plays an important role in the oxygen-dependent killing of tumor cells and microorganisms. MPO deficiency is a hereditary deficiency of the enzyme, which predisposes to immune deficiency. However, elevated MPO levels significantly increases the risk for cardiovascular mortality. The fact that circulating levels of MPO have been shown to predict risks for major adverse cardiac events and that levels of MPO derived chlorinated compounds are specific biomarkers for disease progression, has attracted considerable interest in the development of therapeutic MPO inhibitors.