AB204705

N-Acetylglucosaminidase (beta-NAG) Activity Assay Kit (Colorimetric)

Name: N-Acetylglucosaminidase (beta-NAG) Activity Assay Kit | Abcam
Brand: Abcam Limited
SKU: AB204705
Price: 510 USD
Availability: InStock

Be the first to review this product! Submit a review

(8 Publications)

N-Acetylglucosaminidase (beta-NAG) Activity Assay Kit (Colorimetric) (ab204705) provides a simple and sensitive method for monitoring NAG enzymatic activity.

View Alternative Names

UFHSD1, NAGLU, Alpha-N-acetylglucosaminidase, N-acetyl-alpha-glucosaminidase, NAG

4 Images

Functional Studies - N-Acetylglucosaminidase (beta-NAG) Activity Assay Kit (Colorimetric) (AB204705)

FuncS

Supplier Data

Functional Studies - N-Acetylglucosaminidase (beta-NAG) Activity Assay Kit (Colorimetric) (AB204705)

Typical pNP Standard calibration curve.

FuncS

Supplier Data

Functional Studies - N-Acetylglucosaminidase (beta-NAG) Activity Assay Kit (Colorimetric) (AB204705)

Measurement of NAG activity in human urine from different donors. Undiluted samples (70 μL) were incubated for 30 min. with NAG substrate.

FuncS

Supplier Data

Functional Studies - N-Acetylglucosaminidase (beta-NAG) Activity Assay Kit (Colorimetric) (AB204705)

Measurement of NAG activity in mouse kidney (10 μg) and human serum (20 μL). Samples were incubated for 30 min. with NAG substrate.

Schematic Diagram - N-Acetylglucosaminidase (beta-NAG) Activity Assay Kit (Colorimetric) (AB204705)

Schematic Diagram

Supplier Data

Schematic Diagram - N-Acetylglucosaminidase (beta-NAG) Activity Assay Kit (Colorimetric) (AB204705)

Representative image of N-Acetylglucosaminidase (beta-NAG) Activity Assay Kit (Colorimetric) ab204705

Components shown from left to right :

- Nitrophenol Standard

- NAG Positive Control

- NAG Substrate

- NAG Stop Solution

- NAG Assay Buffer

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Urine, Tissue, Suspension cells, Serum, Adherent cells

Assay type

Enzyme activity

Sensitivity

= 50 µU

Assay time

25m

Assay Platform

Microplate reader

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

N-Acetylglucosaminidase (beta-NAG) Activity Assay Kit (Colorimetric) (ab204705) provides a simple and sensitive method for monitoring NAG enzymatic activity.

How the assay works
In the N-Acetylglucosaminidase (beta-NAG) assay, NAG uses a synthetic substrate, like p-nitrophenyl-N-acetyl-beta-D-glucosaminide (pNP-GlcNAc), to release a detectable product, p-nitrophenol (pNP), which can be measured by absorbance (400 nm).

N-Acetylglucosaminidase (beta-NAG) assay protocol summary
- Add samples and standards to wells
- Add NAG Substrate to sample wells and incubate for 5-30 minutes
- Add Stop Solution and incubate for 10 minutes
- Analyze with a microplate reader to measure optical density (OD 400 nm).

How other researchers are using ab204705
N-Acetylglucosaminidase (beta-NAG) Activity Assay Kit (Colorimetric) has been used with a variety of sample types including:
Human iPSC-derived lacrimal glands ¹
Calf adipocytes ²
Blood and urine sample from diabetic mice ³
References: 1-Asal M et al. 2023, 2-Yu H et al. 2023, 3-de Groot T et al. 2023

Other Notes
This product was previously called K733 Biovision Beta-N-Acetylglucosaminidase Activity Assay Kit (Colorimetric). Biovision was acquired by Abcam in 2021.

Beta-N-Acetylglucosaminidase (NAG, EC 3.2.1.52) is a lysosomal enzyme that is expressed in various tissues, including kidney, liver and lungs. NAG can cleave N-acetyl-glucosamine, a monosaccharide derivative of glucose. Its concentration in urine is minimal due to its inability to cross the glomerular basal membrane. Increased concentration of NAG in urine indicates renal tubular cell breakdown. Acute Kidney Injury (AKI) is the sudden loss of kidney functions, causing electrolyte imbalance, and retention of urea and other nitrogenous products. NAG has become one of the most studied and used biomarkers for the detection and diagnosis of AKI.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

style

left-column

What's included?

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab204705-100Test", "assetComponentDetails": [ { "size":"1 x 400 µL", "name":"Nitrophenol Standard", "number":"AB204705-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"NAG Substrate", "number":"AB204705-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"NAG Positive Control", "number":"AB204705-CMP03", "productcode":"" }, { "size":"1 x 35 mL", "name":"NAG Assay Buffer", "number":"AB204705-CMP02", "productcode":"" }, { "size":"1 x 3 mL", "name":"NAG Stop Solution", "number":"AB204705-CMP04", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N-Acetylglucosaminidase also known as NAG β-NAG and NAG-5 plays an important role in the breakdown of glycoproteins by catalyzing the hydrolysis of terminal N-acetylglucosamine residues in N-acetyl-β-D-glucosaminides. This enzyme has a molecular mass of approximately 55 kDa. It expresses in various tissues with noticeable activity in lysosomes and is critical for maintaining cellular homeostasis by processing glycosylated proteins.

Biological function summary

The enzyme N-acetylglucosaminidase is essential for cell growth signaling and structural integrity. It belongs to a larger group known as glycoside hydrolases which cooperate during the degradation of complex carbohydrates and glycoproteins. NAG's function impacts the lysosomal degradation pathway where it often works with substrates like glycosaminoglycans ensuring the recycling of cellular components in the lysosomal environment.

Pathways

N-acetylglucosaminidase integrates with lysosomal storage pathways and takes part in glycosaminoglycan degradation. Its activity is keenly tied with proteins such as β-glucuronidase working alongside in the sequential breakdown of glycan chains. These pathways are important for cellular waste disposal and recycling contributing to overall metabolic harmony in cells.

N-acetylglucosaminidase links to conditions such as mucopolysaccharidosis and Tay-Sachs disease. Deficiency in NAG enzymatic function leads to improper degradation of glycosaminoglycans or gangliosides contributing to their pathophysiology. The enzyme shares this relation with proteins like Hexosaminidase A in Tay-Sachs disease demonstrating its pivotal role in neurological health.

style

left-column

Product protocols

style

left-column

Target data

Involved in the degradation of heparan sulfate.

See full target information NAGLU

style

left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39684207

2024

Endothelial Dysfunction and Impaired Wound Healing Following Radiation Combined Skin Wound Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Li Wang,Bin Lin,Min Zhai,Lisa Hull,Wanchang Cui,Mang Xiao

Nature communications 14:4890 PubMed37644033

2023

Feasibility of an implantable bioreactor for renal cell therapy using silicon nanopore membranes.

Applications

Unspecified application

Species

Unspecified reactive species

Eun Jung Kim,Caressa Chen,Rebecca Gologorsky,Ana Santandreu,Alonso Torres,Nathan Wright,Mark S Goodin,Jarrett Moyer,Benjamin W Chui,Charles Blaha,Paul Brakeman,Shant Vartanian,Qizhi Tang,H David Humes,William H Fissell,Shuvo Roy

Frontiers in cell and developmental biology 10:1058846 PubMed36684423

2023

Development of lacrimal gland organoids from iPSC derived multizonal ocular cells.

Applications

Unspecified application

Species

Unspecified reactive species

Melis Asal,Gamze Koçak,Vedat Sarı,Tuba Reçber,Emirhan Nemutlu,Canan Aslı Utine,Sinan Güven

Journal of dairy science 105:6997-7010 PubMed35688731

2022

Activated autophagy-lysosomal pathway in dairy cows with hyperketonemia is associated with lipolysis of adipose tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Yu,Minghe Fan,Xiying Chen,Xiuhuan Jiang,Juan J Loor,Ahmad Aboragah,Cai Zhang,Hongxu Bai,Zhiyuan Fang,Taiyu Shen,Zhe Wang,Yuxiang Song,Xinwei Li,Guowen Liu,Xiaobing Li,Xiliang Du

Environmental science and pollution research international 29:52655-52664 PubMed35274206

2022

Sex-specific differences in early renal impairment associated with arsenic, lead, and cadmium exposure among young adults in Taiwan.

Applications

Unspecified application

Species

Unspecified reactive species

Kai-Wei Liao,Ling-Chu Chien,Yang-Ching Chen,Ho-Ching Kao

3 Biotech 9:66 PubMed30729090

2019

Urinary -acetyl-beta-d-glucosaminidase (NAG) with neutrophil gelatinase-associated lipocalin (NGAL) improves the diagnostic value for proximal tubule damage in diabetic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Khalid Siddiqui,Basim Al-Malki,Teena Puthiyaparampil George,Shaik Sarfaraz Nawaz,Khalid Al Rubeaan

PloS one 12:e0189485 PubMed29244860

2017

Lithium reduces blood glucose levels, but aggravates albuminuria in BTBR-ob/ob mice.

Applications

Unspecified application

Species

Unspecified reactive species

Theun de Groot,Lars Damen,Leanne Kosse,Mohammad Alsady,Rosalinda Doty,Ruben Baumgarten,Susan Sheehan,Johan van der Vlag,Ron Korstanje,Peter M T Deen

ASAIO journal (American Society for Artificial Internal Organs : 1992) 64:766-772 PubMed29240625

2017

Orbital Shear Stress Regulates Differentiation and Barrier Function of Primary Renal Tubular Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Ferrell,Jin Cheng,Simeng Miao,Shuvo Roy,William H Fissell

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

N-Acetylglucosaminidase (beta-NAG) Activity Assay Kit (Colorimetric)

Functional Studies - N-Acetylglucosaminidase (beta-NAG) Activity Assay Kit (Colorimetric) (AB204705)

Functional Studies - N-Acetylglucosaminidase (beta-NAG) Activity Assay Kit (Colorimetric) (AB204705)

Functional Studies - N-Acetylglucosaminidase (beta-NAG) Activity Assay Kit (Colorimetric) (AB204705)

Schematic Diagram - N-Acetylglucosaminidase (beta-NAG) Activity Assay Kit (Colorimetric) (AB204705)

Key facts

Detection method

Sample types

Assay type

Sensitivity

Assay time

Assay Platform

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Complementary Products

Secondary Antibodies

Assay Kits

Assay Kits

Assay Kits

Product promise