N-Acetylglucosaminidase (beta-NAG) Activity Assay Kit (Colorimetric) (ab204705) provides a simple and sensitive method for monitoring NAG enzymatic activity.N-Acetylglucosaminidase (beta-NAG) Activity Assay Kit (Colorimetric)designed for success:
- Can be used with a variety of sample types, including cell lysates, tissue extracts, body fluids
- Highly sensitive, of which the detection limit can be as low as 50 µU of beta-NAG activity
- Simple protocol and fast results obtained in less than one hour
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Publications
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- Nature communications 14:48902023Feasibility of an implantable bioreactor for renal cell therapy using silicon nanopore membranes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEun Jung Kim,Caressa Chen,Rebecca Gologorsky,Ana Santandreu,Alonso Torres,Nathan Wright,Mark S Goodin,Jarrett Moyer,Benjamin W Chui,Charles Blaha,Paul Brakeman,Shant Vartanian,Qizhi Tang,H David Humes,William H Fissell,Shuvo RoyPubMed 37644033
- Frontiers in cell and developmental biology 10:10588462023Development of lacrimal gland organoids from iPSC derived multizonal ocular cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMelis Asal,Gamze Koçak,Vedat Sarı,Tuba Reçber,Emirhan Nemutlu,Canan Aslı Utine,Sinan GüvenPubMed 36684423
- Journal of dairy science 105:6997-70102022Activated autophagy-lysosomal pathway in dairy cows with hyperketonemia is associated with lipolysis of adipose tissues.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao Yu,Minghe Fan,Xiying Chen,Xiuhuan Jiang,Juan J Loor,Ahmad Aboragah,Cai Zhang,Hongxu Bai,Zhiyuan Fang,Taiyu Shen,Zhe Wang,Yuxiang Song,Xinwei Li,Guowen Liu,Xiaobing Li,Xiliang DuPubMed 35688731
- Environmental science and pollution research international 29:52655-526642022Sex-specific differences in early renal impairment associated with arsenic, lead, and cadmium exposure among young adults in Taiwan.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai-Wei Liao,Ling-Chu Chien,Yang-Ching Chen,Ho-Ching KaoPubMed 35274206
- 3 Biotech 9:662019Urinary -acetyl-beta-d-glucosaminidase (NAG) with neutrophil gelatinase-associated lipocalin (NGAL) improves the diagnostic value for proximal tubule damage in diabetic kidney disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKhalid Siddiqui et. al.PubMed 30729090
- PloS one 12:e01894852017Lithium reduces blood glucose levels, but aggravates albuminuria in BTBR-ob/ob mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTheun de Groot et. al.PubMed 29244860
- ASAIO journal (American Society for Artificial Internal Organs : 1992) 64:766-7722017Orbital Shear Stress Regulates Differentiation and Barrier Function of Primary Renal Tubular Epithelial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicholas Ferrell,Jin Cheng,Simeng Miao,Shuvo Roy,William H FissellPubMed 29240625
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Tissue, Suspension cells, Serum, Adherent cells
- Assay type
- Enzyme activity
- Reactive species
- Mammals
- Assay time
- 25m
- Sensitivity
- = 50 µU
Alternative names
UFHSD1, NAGLU, Alpha-N-acetylglucosaminidase, N-acetyl-alpha-glucosaminidase, NAG
What's included?
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N-Acetylglucosaminidase (beta-NAG) Activity Assay Kit (Colorimetric) (ab204705) provides a simple and sensitive method for monitoring NAG enzymatic activity.N-Acetylglucosaminidase (beta-NAG) Activity Assay Kit (Colorimetric)designed for success:
- Can be used with a variety of sample types, including cell lysates, tissue extracts, body fluids
- Highly sensitive, of which the detection limit can be as low as 50 µU of beta-NAG activity
- Simple protocol and fast results obtained in less than one hour
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Tissue, Suspension cells, Serum, Adherent cells
- Assay type
- Enzyme activity
- Reactive species
- Mammals
- Assay time
- 25m
- Assay Platform
- Microplate reader
- Sensitivity
- = 50 µU
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
How the assay works
In N-Acetylglucosaminidase
(beta-NAG) Activity Assay Kit, NAG uses a synthetic substrate, like p-nitrophenyl-N-acetyl-beta-D-glucosaminide (pNP-GlcNAc), to release a detectable product, p-nitrophenol (pNP), which can be measured at absorbance (OD 400 nm) based on its absorbance change.
N-Acetylglucosaminidase (beta-NAG) Activity Assay Kit (Colorimetric) protocol summary:
- Add samples and standards to wells
- Add NAG Substrate to sample wells and incubate for 5 – 30 minutes
- Add Stop Solution and incubate for 10 minutes
- Analyze with a microplate reader to measure optical density (OD 400 nm).
How other researchers are using
N-Acetylglucosaminidase (beta-NAG) Activity Assay Kit (Colorimetric) has been used in a variety of sample type including:
Human iPSC-derived lacrimal glands 1
Calf adipocytes 2
Blood and urine sample from diabetic mice 3
References:
1-Asal M et al. 2023
2-Yu H et al. 2023
3-de Groot T et al. 2023
This product is manufactured by BioVision, an Abcam company and was previously called K733 β-N-Acetylglucosaminidase Activity Assay Kit (Colorimetric). K733-100 is the same size as the 100 test size of ab204705.
β-N-Acetylglucosaminidase (NAG, EC 3.2.1.52) is a lysosomal enzyme that is expressed in various tissues, including kidney, liver and lungs. NAG can cleave N-acetyl-glucosamine, a monosaccharide derivative of glucose. Its concentration in urine is minimal due to its inability to cross the glomerular basal membrane. Increased concentration of NAG in urine indicates renal tubular cell breakdown. Acute Kidney Injury (AKI) is the sudden loss of kidney functions, causing electrolyte imbalance, and retention of urea and other nitrogenous products. NAG has become one of the most studied and used biomarkers for the detection and diagnosis of AKI.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
N-Acetylglucosaminidase also known as NAG β-NAG and NAG-5 plays an important role in the breakdown of glycoproteins by catalyzing the hydrolysis of terminal N-acetylglucosamine residues in N-acetyl-β-D-glucosaminides. This enzyme has a molecular mass of approximately 55 kDa. It expresses in various tissues with noticeable activity in lysosomes and is critical for maintaining cellular homeostasis by processing glycosylated proteins.
- Biological function summary
The enzyme N-acetylglucosaminidase is essential for cell growth signaling and structural integrity. It belongs to a larger group known as glycoside hydrolases which cooperate during the degradation of complex carbohydrates and glycoproteins. NAG's function impacts the lysosomal degradation pathway where it often works with substrates like glycosaminoglycans ensuring the recycling of cellular components in the lysosomal environment.
- Pathways
N-acetylglucosaminidase integrates with lysosomal storage pathways and takes part in glycosaminoglycan degradation. Its activity is keenly tied with proteins such as β-glucuronidase working alongside in the sequential breakdown of glycan chains. These pathways are important for cellular waste disposal and recycling contributing to overall metabolic harmony in cells.
- Associated diseases and disorders
N-acetylglucosaminidase links to conditions such as mucopolysaccharidosis and Tay-Sachs disease. Deficiency in NAG enzymatic function leads to improper degradation of glycosaminoglycans or gangliosides contributing to their pathophysiology. The enzyme shares this relation with proteins like Hexosaminidase A in Tay-Sachs disease demonstrating its pivotal role in neurological health.
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3 product images
Functional Studies - N-Acetylglucosaminidase (beta-NAG) Activity Assay Kit (Colorimetric) (ab204705)
Typical pNP Standard calibration curve.
Functional Studies - N-Acetylglucosaminidase (beta-NAG) Activity Assay Kit (Colorimetric) (ab204705)
Measurement of NAG activity in human urine from different donors. Undiluted samples (70 μL) were incubated for 30 min. with NAG substrate.
Functional Studies - N-Acetylglucosaminidase (beta-NAG) Activity Assay Kit (Colorimetric) (ab204705)
Measurement of NAG activity in mouse kidney (10 μg) and human serum (20 μL). Samples were incubated for 30 min. with NAG substrate.
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