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AB238540

N-epsilon-(Carboxyethyl) Lysine (CEL) Assay Kit

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N-epsilon-(Carboxyethyl) Lysine (CEL) Assay Kit (ab238540) is designed for the rapid detection and quantitation of CEL protein adducts.
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Competitive ELISA - N-epsilon-(Carboxyethyl) Lysine (CEL) Assay Kit (AB238540)
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Supplier Data

Competitive ELISA - N-epsilon-(Carboxyethyl) Lysine (CEL) Assay Kit (AB238540)

Example CEL-BSA ELISA Standard Curve.

Typical CEL ELISA results.

This data is for reference only and should not be used to interpret actual results.

Key facts

Detection method

Colorimetric

Sample types

Purified protein, Plasma, Serum, Cell Lysate

Assay type

Competitive

Range

0.1 - 25 µg/mL

Assay Platform

Microplate reader

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Product details

N-epsilon-(Carboxyethyl) Lysine (CEL) Assay Kit (ab238540) is designed for the rapid detection and quantitation of CEL protein adducts.

First, a CEL conjugate is coated on the ELISA plate. The unknown CEL protein samples or CEL-BSA standards are then added to the CEL conjugate preabsorbed plate. After a brief incubation the anti-CEL monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The content of CEL protein adducts in unknown samples is determined by comparison with the pre-determined CEL-BSA standard curve. Despite the structure similarity between CEL and CML, the anti-CEL specific antibody in this kit will not cross react with CML protein adducts.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N-epsilon-(Carboxyethyl) Lysine (CEL) also known as carboxyethyl lysine stands as a specific type of advanced glycation end product (AGE). It forms through the non-enzymatic glycation of proteins particularly lysine residues. CEL exhibits a variable mass depending on its attached protein matrix. Researchers identify its presence significantly in long-lived proteins such as collagen and in dietary products like dairy and roasted meats. CEL forms through Maillard reaction pathways creating cross-links in proteins which affect their structure and function.
Biological function summary

The modification of proteins by CEL impacts cellular processes. CEL alters the function and lifespan of proteins disrupting homeostasis and contributing to altered cell signaling pathways. It is associated with the alteration of extracellular matrix proteins and redox-sensitive cytoplasmic proteins. CEL is not typically part of a specific complex but its presence can influence the assembly and function of protein complexes within cells. Its influence on protein modification underlies the aging process and other cellular dysfunctions.

Pathways

CEL plays a role in oxidative stress and inflammatory pathways. It associates with the receptor for AGE (RAGE) initiating signaling cascades that contribute to inflammation and oxidative stress. The AGE-RAGE axis represents a fundamental pathway where CEL engages in modifying cellular responses and protein expressions. Other related proteins include glyoxalase enzymes that detoxify reactive carbonyl species thereby potentially counteracting CEL formation and mitigating downstream effects.

CEL contributes to the pathogenesis of diabetes and atherosclerosis among others. In diabetes CEL accumulates in vascular tissues affecting hemodynamic function and promoting complications via RAGE-related pathways. In atherosclerosis CEL-modified proteins in arterial walls favor progression due to heightened inflammation and endothelial cell dysfunction. This interaction with RAGE and other AGE-related proteins assists in understanding CEL's role in disease progression and highlights potential therapeutic targets for managing these chronic conditions.
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Product protocols

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Target data

See full target information N(6)-(1-carboxyethyl)-L-lysine
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websiteProtocolBooklet
en
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Product promise

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