Skip to main content

NAD/NADH Assay Kit (Colorimetric) ab65348 provides a convenient and sensitive tool to quantify NAD+ and NADH, and measure their ratio, in samples from mammals and other species.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

This product has no reviews yet! Submit a review
Detection method

Colorimetric

Sample types

Tissue Lysate, Urine, Serum, Cell Lysate

Assay type

Quantitative

Range

400 - 2000 nM

Assay time

2h

Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

Images

What's included?

2000 Test
Components
Cycling Buffer I
20 x 15 mL
Developer Solution II
20 x 1 Vial
Extraction Buffer II
20 x 50 mL
NAD Cycling Enzyme Mix
20 x 1 Vial
NADH Standard II
20 x 1 Vial
Stop Solution II
20 x 1.2 mL

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

NAD/NADH Assay Kit (Colorimetric) ab65348 provides a convenient and sensitive tool to quantify NAD+ and NADH, and measure their ratio, in samples from mammals and other species.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate, Urine, Serum, Cell Lysate

Assay type

Quantitative

Range

400 - 2000 nM

Assay time

2h

Assay Platform

Microplate reader

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

NAD/NADH Assay Kit (Colorimetric) ab65348 provides a convenient and sensitive tool to quantify NAD+ and NADH, and measure their ratio, in samples from mammals and other species.

The NAD cycling enzyme mix in the kit specifically acts on NADH/NAD in an enzyme cycling reaction which significantly increases sensitivity and specificity. There is no requirement to purify NADH/NAD from samples.

The levels of both NADt (total NAD+ and NADH) and NADH can be easily measured; the level of NAD+ can be easily calculated by subtracting NADH from NADt. The assay is read by absorbance at 450 nm.

NAD / NADH assay protocol summary:
- extract samples from cells / tissues with extraction buffer and deproteinize with spin column
- for NADH measurement, heat samples to 60°C for 30 min to decompose NAD+, cool on ice (this step not necessary for measurement of total NAD+/NADH)
- add samples and standards to wells
- add reaction mix and incubate for 5 min at room temp to convert NAD to NADH
- add NADH developer and incubate for 1-4 hrs while reaction cycles
- analyze with microplate reader multiple times during the 1-4 hr incubation
- reaction can be stopped with stop solution.

This assay specifically detects NAD and NADH, but not NADP nor NADPH. If you would like to use a fluorometric reading, please refer to NAD/NADH Assay Kit (Fluorometric) (ab176723). NAD/NADH Assay kit ab221821 uses an alternative assay method that relies on purification of NAD and NADH from samples and may be more sensitive in some samples. Review our to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader. **How other researchers have used NAD/NADH Assay Kit ab65348** This NAD/NADH assay kit has been used in publications in a variety of sample types, including: - Human: primary blood mononuclear cells1, epithelial ovarian cancer cells2, Jurkat cells3 - Mouse: cell culture lysates4, cardiomyocyte cell culture lysates5, liver6, liver and muscle7, primary hepatocyte cell cultures8, aorta tissue9 - Rat: brain tissue10 - Locust: thoraic muscle11 Bacteria: Z mobilis12, E coli13 References: 1 - Castro-Marrero J et al 2015; 2 - Zhu J et al 2019, Xia H et al 2015; 3 - Miller TW et al 2015; 4 - Mekala NK et al 2019, Ling S et al 2017; 5 - Zhang D et al 2019; 6 - Shao D et al 2017, Mukherji A et al 2015, Yu JH et al 2016; 7 - Karandrea S et al 2017; 8 - Traboulsi H et al 2014; 9 - Liu Y et al 2016; 10 - Rao G et al 2016; 11 - Ding D et al 2018; 12 - Wu B et al 2019; 13 - Long YM et al 2017

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com