JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB228548

NADP Regeneration Kit

Be the first to review this product! Submit a review

|

(0 Publication)

NADP Regeneration Kit (ab228548) provides two ready to use solutions to regenerate NADP by a simple mixing.

Key facts

style
left-column

Product details

NADP Regeneration Kit (ab228548) provides two ready to use solutions to regenerate NADP by a simple mixing. This kit can be used for all NADP-requiring systems (e.g. 12-Ketochenodeoxycholic Acid synthesis, Ferredoxin Reductase System). About 300-500 enzyme assays can be performed using this kit. The total number of assays that can be performed depends on the experimental design.

NADP is the electron acceptor for biosynthetic reactions and dehydrogenation reactions. NADP is used for anabolic pathways, such as lipid synthesis, cholesterol synthesis and fatty acid chain elongation. It is also a necessary cofactor in many xenobiotic metabolism reactions. In chloroplasts, NADP is reduced to NADPH by ferredoxin-NADP reductase in the last step of the electron chain in photosynthesis reactions. Many oxidoreductases and all ligases use NADP/NADPH as coenzyme. NADP can also be used in the determination of amylase, creatine kinase, glucose-6-phosphate dehydrogenase, isocitrate dehydrogenase.

style
left-column

What's included?

{ "values": { "200Test": { "sellingSize": "200 Test", "publicAssetCode":"ab228548-200Test", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"NADP Regenerating Buffer II", "number":"AB228548-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"NADP Regenerating Buffer I", "number":"AB228548-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"NADP Regenerating Enzyme Mix", "number":"AB228548-CMP04", "productcode":"" }, { "size":"1 x 2 Vial", "name":"NADPH", "number":"AB228548-CMP01", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NADP/NADPH also known as Nicotinamide Adenine Dinucleotide Phosphate and its reduced form plays an essential role in cellular metabolism. It is a coenzyme with a molecular mass of around 743.4 g/mol. NADP/NADPH is ubiquitously expressed in cells due to its integral role in metabolic processes. It is found in high concentrations within the cytoplasm and chloroplasts where it functions in various biochemical reactions.
Biological function summary

NADPH facilitates reductive biosynthesis and maintains cellular redox status. It acts as a major electron donor in various anabolic reactions including lipid and nucleic acid synthesis. NADPH also contributes to the detoxification of reactive oxygen species and serves in the process of oxidative phosphorylation. Its cofactor activity associates it predominantly with enzymatic complexes such as the cytochrome P450 system.

Pathways

NADPH is fundamental in both the pentose phosphate pathway and the Calvin cycle. In the pentose phosphate pathway it provides reducing power important for fatty acid and cholesterol biosynthesis. In photosynthetic organisms NADPH functions in the Calvin cycle facilitating carbon fixation. The enzyme glucose-6-phosphate dehydrogenase related to NADPH production is integral in NADPH recycling.

Impaired NADPH function links to conditions like chronic granulomatous disease and diabetes. Chronic granulomatous disease occurs due to defects in the NADPH oxidase complex impairing the body’s ability to combat pathogens effectively. Diabetes has associations with oxidative stress influenced by imbalance in NADPH levels. In these diseases NADPH's interaction with proteins like superoxide dismutase affects oxidative damage management and overall immune response.
style
left-column

Product protocols

style
left-column

Target data

See full target information Triphosphopyridine nucleotide
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB228547

NADPH Regeneration Kit

  • 0
  • 0
Assay Kits

AB65349

NADP/NADPH Assay Kit

Functional Studies - NADP/NADPH Assay Kit (AB65349)

  • 4
  • 1
Assay Kits

AB186033

Total NADP and NADPH Assay Kit (Colorimetric)

Functional Studies - Total NADP and NADPH Assay Kit (Colorimetric) (AB186033)

  • 0
  • 0
Assay Kits

AB204718

Succinate Assay Kit (Colorimetric)

Functional Studies - Succinate Assay Kit (Colorimetric) (AB204718)

  • 5
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com