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AB235979

NETosis Assay Kit

5

(1 Review)

|

(2 Publications)

NETosis Assay Kit ab235979 is used to assay NETosis in primary neutrophils.

- Complete kit for stimulation and measurement of NETosis
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Functional Studies - NETosis Assay Kit (AB235979)
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Supplier Data

Functional Studies - NETosis Assay Kit (AB235979)

Human neutrophil elastase standard curve.

Functional Studies - NETosis Assay Kit (AB235979)
  • FuncS

Supplier Data

Functional Studies - NETosis Assay Kit (AB235979)

Measurement of released neutrophil elastase parallels measurement of released dsDNA.

Human neutrophils were treated with PMA for 4 hours, washed and treated with S7 Nuclease for 15 minutes. The supernatant from each well was sampled and assayed for neutrophil elastase according to the protocol booklet. The supernatant was also tested for the prescence of soluble dsDNA using a green fluorescence probe.

Key facts

Detection method

Colorimetric

Sample types

Suspension cells

Reacts with

Human

Assay type

Quantitative

Range

0.56 - 36 mU/well

Assay Platform

Microplate reader

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Product details

NETosis Assay Kit (ab235979) provides a simple and fast method for studying the process of NETosis ex vivo.

In this kit, primary neutrophils are stimulated to release NETs with either PMA or a calcium ionophore (both included). Unbound neutrophil elastase is washed away following NET generation. Following digest of NET DNA by S7 nuclease, the supernatant containing neutrophil elastase is added to a substrate, which is selectively cleaved by elastase to yield a 4-nitroaniline product that adsorbs light at 405 nm. Enough reagents are provided to test 24 sample conditions for NET production, with analysis in duplicate.

Note: This kit does not depend upon the DNA component of neutrophil extracellular traps (NETs), as DNA release can occur independently of NETosis.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Product protocols

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Target data

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Life (Basel, Switzerland) 13: PubMed38137854

2023

Tacrolimus Maintains the Balance of Neutrophil Extracellular Traps by Inducing DNA Methylation of Neutrophils to Reduce Immune Rejection.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Xu,Ming Cai

Haematologica 107:2445-2453 PubMed35385923

2022

The deglycosylated form of 1E12 inhibits platelet activation and prothrombotic effects induced by VITT antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline Vayne,Raghavendra Palankar,Sandra Billy,Stefan Handtke,Thomas Thiele,Charlotte Cordonnier,Claire Pouplard,Andreas Greinacher,Yves Gruel,Jérôme Rollin
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