Neutrophil Elastase Inhibitor Screening Kit (ab118971) is a rapid and simple assay to screen neutrophil elastase inhibitors. Readout on any fluorometric (Ex/Em = 400/505) plate reader.
- Kit includes assay buffer, elastase enzyme, inhibitor control and enzyme substrate
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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- International journal of molecular sciences 24:2023Comparative Study of Cytotoxicity and Antioxidant, Anti-Aging and Antibacterial Properties of Unfermented and Fermented Extract of L.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMartyna Zagórska-Dziok,Aleksandra Ziemlewska,Agnieszka Mokrzyńska,Zofia Nizioł-Łukaszewska,Ireneusz Sowa,Dariusz Szczepanek,Magdalena WójciakPubMed 37686038
- BioFactors (Oxford, England) 49:1038-10602023Skincare potential of a sustainable postbiotic extract produced through sugarcane straw fermentation by Saccharomyces cerevisiae.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarco Duarte,Maria João Carvalho,Nelson Mota de Carvalho,João Azevedo-Silva,Adélia Mendes,Inês Pinto Ribeiro,João Carlos Fernandes,Ana L S Oliveira,Carla Oliveira,Manuela Pintado,Ana Amaro,Ana Raquel MadureiraPubMed 37317790
- Applied and environmental microbiology 89:e00335232023Export of Diverse and Bioactive Small Proteins through a Type I Secretion System.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSun-Young Kim,Jennifer K Parker,Monica Gonzalez-Magaldi,Mady S Telford,Daniel J Leahy,Bryan W DaviesPubMed 37078870
- Molecules (Basel, Switzerland) 28:2023Potential Biological Properties of Lycopene in a Self-Emulsifying Drug Delivery System.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSônia Nair Báo,Manuela Machado,Ana Luisa Da Silva,Adma Melo,Sara Cunha,Sérgio S Sousa,Ana Rita Malheiro,Rui Fernandes,José Roberto S A Leite,Andreanne G Vasconcelos,João Relvas,Manuela PintadoPubMed 36770886
- Molecules (Basel, Switzerland) 28:2023Phenolic Profile and Comparison of the Antioxidant, Anti-Ageing, Anti-Inflammatory, and Protective Activities of Extracts on Skin Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMonika Michalak,Martyna Zagórska-Dziok,Marta Klimek-Szczykutowicz,Agnieszka SzopaPubMed 36677923
- Molecules (Basel, Switzerland) 27:2022Evaluation of Cosmetic and Dermatological Properties of Kombucha-Fermented Berry Leaf Extracts Considered to Be By-Products.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAleksandra Ziemlewska,Zofia Nizioł-Łukaszewska,Martyna Zagórska-Dziok,Tomasz Bujak,Magdalena Wójciak,Ireneusz SowaPubMed 35408743
- Molecules (Basel, Switzerland) 27:2022Flower Extracts as Multifunctional Dyes in the Cosmetics Industry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomasz Bujak,Martyna Zagórska-Dziok,Aleksandra Ziemlewska,Zofia Nizioł-Łukaszewska,Kamila Lal,Tomasz Wasilewski,Zofia Hordyjewicz-BaranPubMed 35164187
- Scientific reports 11:187922021Effect of fermentation time on the content of bioactive compounds with cosmetic and dermatological properties in Kombucha Yerba Mate extracts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAleksandra Ziemlewska,Zofia Nizioł-Łukaszewska,Tomasz Bujak,Martyna Zagórska-Dziok,Magdalena Wójciak,Ireneusz SowaPubMed 34552134
- Molecules (Basel, Switzerland) 26:2021L. Extracts as Valuable Ingredients in Cosmetic and Dermatological Products.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Nowak,Martyna Zagórska-Dziok,Paula Ossowicz-Rupniewska,Edyta Makuch,Wiktoria Duchnik,Łukasz Kucharski,Urszula Adamiak-Giera,Piotr Prowans,Norbert Czapla,Piotr Bargiel,Jan Petriczko,Marta Markowska,Adam KlimowiczPubMed 34200200
- Molecules (Basel, Switzerland) 26:2021Antioxidant and Cytoprotective Properties of Plant Extract from Dry Flowers as Functional Dyes for Cosmetic Products.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomasz Bujak,Martyna Zagórska-Dziok,Aleksandra Ziemlewska,Zofia Nizioł-Łukaszewska,Tomasz Wasilewski,Zofia Hordyjewicz-BaranPubMed 34068613
Key facts
- Detection method
- Fluorescent
- Sample types
- Test Ligands
- Assay type
- Quantitative
- Assay time
- 40m
Target data
Function
Serine protease that modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed:15140022). Promotes cleavage of GSDMB, thereby inhibiting pyroptosis (PubMed:36899106). Promotes blood coagulation (PubMed:20676107). Through the activation of the platelet fibrinogen receptor integrin alpha-IIb/beta-3, potentiates platelet aggregation induced by a threshold concentration of cathepsin G (CTSG) (PubMed:25211214, PubMed:9111081). Cleaves and thus inactivates tissue factor pathway inhibitor (TFPI) (PubMed:20676107, PubMed:25211214). Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae (PubMed:10947984).
Alternative names
ELA2, ELANE, Neutrophil elastase, Bone marrow serine protease, Elastase-2, Human leukocyte elastase, Medullasin, PMN elastase, HLE
What's included?
Recommended products
Neutrophil Elastase Inhibitor Screening Kit (ab118971) is a rapid and simple assay to screen neutrophil elastase inhibitors. Readout on any fluorometric (Ex/Em = 400/505) plate reader.
- Kit includes assay buffer, elastase enzyme, inhibitor control and enzyme substrate
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
- Detection method
- Fluorescent
- Sample types
- Test Ligands
- Assay type
- Quantitative
- Assay time
- 40m
- Assay Platform
- Microplate reader
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
How the assay works
In Neutrophil Elastase Inhibitor Assay Kit protocol, Neutrophil Elastase hydrolyzes a specific fluorescent substrate to release the fluorescent group, which can be detected at Ex/Em = 400/505 nm. In presence of a potent neutrophil elastase inhibitor, the hydrolyzation of substrate will be inhibited or stopped. For comparison of the relative efficacy of test inhibitors, a control inhibitor, SPCK (Ki = 10 µM for human leukocyte elastase) is included.
Neutrophil Elastase Inhibitor Assay Kit protocol summary
- Test inhibitor preparation Neutrophil elastase preparation
- Incubate for 5 mins at 37°C
- Add substrate mix
- Measure fluorescence (Ex/Em = 400/505 nm) in a kinetic mode
- Incubate for 30 mins at 37°C protected from light
- Measure fluorescence (Ex/Em = 400/505 nm) in a kinetic mode
Neutrophil Elastase Inhibitor Assay Kit has been used in a variety of sample type including:
C. mas L. extracts 1
B. officinalis herb extracts 2
Psidium guajava L. extracts 3
References:
1-Zagуrska-Dziok et al. 2023
2-Michalak M al. 2023
3-Bao S et al. 2023
This product is manufactured by BioVision, an Abcam company and was previously called K782 Neutrophil Elastase Inhibitor Screening Kit (Fluorometric). K782-100 is the same size as the 100 test size of ab118971.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Neutrophil Elastase also known as neutrophil proteinase 3 has a molecular mass of approximately 30 kDa. It is a serine protease expressed mainly in azurophilic granules of neutrophils. This enzyme breaks down various proteins including elastin at sites of inflammation. Neutrophil Elastase releases during degranulation or through neutrophil extracellular traps (NETs) to combat pathogens.
- Biological function summary
The target plays a significant role in neutrophil-mediated tissue remodeling and host defense. It leads to the breaking down of extracellular matrix components contributing to tissue remodeling and repair. Neutrophil Elastase is part of a complex interplay with other proteases like matrix metalloproteinases (MMPs). Its activity is regulated by endogenous inhibitors such as alpha-1 antitrypsin to prevent excessive tissue damage.
- Pathways
This protease is important in inflammation and immune response pathways. It plays a role in the complement system by activating C5 a component of the complement cascade. It also acts in conjunction with other proteases such as cathepsin G to modulate immune and inflammatory responses. Neutrophil Elastase's role within these pathways is pivotal for the regulation of inflammation and destruction of pathogens.
- Associated diseases and disorders
Individuals with conditions such as chronic obstructive pulmonary disease (COPD) and cystic fibrosis often exhibit dysregulation or excessive activity of Neutrophil Elastase. This excessive activity leads to tissue damage and exacerbates disease progression. The enzyme also interacts with proteins like elastin and collagen in these diseases further contributing to pathological tissue remodeling and damage.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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4 product images
Functional Studies - Neutrophil Elastase Inhibitor Assay Kit (ab118971)
Neutrophil Elastase Inhibition by SPCK
Functional Studies - Neutrophil Elastase Inhibitor Assay Kit (ab118971)
Neutrophil Elastase Activity Profile at Various Concentrations of Elafin/Skalp (Recombinant Human Elafin/ESI protein ab153768)
Functional Studies - Neutrophil Elastase Inhibitor Assay Kit (ab118971)
Neutrophil Elastase Activity Profile at Various Concentrations of SERPINB1 (Recombinant Human SERPINB1/PI2 protein ab114774)
Functional Studies - Neutrophil Elastase Inhibitor Assay Kit (ab118971)
Neutrophil Elastase Activity Profile at Various Concentrations of SPCK
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