Skip to main content

NFkB p65 Transcription Factor Assay Kit ab133112 is a non-radioactive, sensitive ELISA-based method for detecting specific transcription factor DNA binding activity in nuclear extracts.

Detection method

Colorimetric

Sample types

Nuclear Extracts

Assay type

Semi-quantitative

Reactive species

Mouse, Rat, Human

Assay time

3h 30m

Images

Target data

What's included?

96 Test
Components
96-Well Plate Cover
1 x 1 Unit
Polysorbate 20
1 x 1 Vial
Transcription Factor Antibody Binding Buffer (10X)
1 x 3 mL
Transcription Factor Binding Assay Buffer (4X)
1 x 3 mL
Transcription Factor Developing Solution
1 x 12 mL
Transcription Factor Goat Anti-Rabbit HRP Conjugate
1 x 100 µL
Transcription Factor NF-kB 96-Well Strip Plate
1 x 1 Unit
Transcription Factor NFkB (Human p65) Positive Control
1 x 1 Vial
Transcription Factor NFkB (p65) Primary Antibody
1 x 1 Vial
Transcription Factor NFkB Competitor dsDNA
1 x 1 Vial
Transcription Factor Reagent A
1 x 120 µL
Transcription Factor Stop Solution
1 x 12 mL
Wash Buffer Concentrate (400X)
1 x 5 mL

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

NFkB p65 Transcription Factor Assay Kit ab133112 is a non-radioactive, sensitive ELISA-based method for detecting specific transcription factor DNA binding activity in nuclear extracts.

Key facts

Detection method

Colorimetric

Sample types

Nuclear Extracts

Assay type

Semi-quantitative

Reactive species

Mouse, Rat, Human

Assay time

3h 30m

Assay Platform

Microplate reader

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

Multi

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

NFkB p65 Transcription Factor Assay Kit ab133112 is a non-radioactive, sensitive ELISA-based method for detecting specific transcription factor DNA binding activity in nuclear extracts.

In the NFkB p65 assay, a double stranded DNA sequence containing the NFkB response element is immobilized onto the bottom of the wells of a 96-well plate. NFkB contained in a nuclear extract, binds to the NFkB response element, and is detected using an anti NFkB p65 antibody. A secondary antibody conjugated to HRP is added to provide a colorimetric readout at 450 nm.

NFkB p65 transcription factor assay protocol summary:
- prepare nuclear extracts from cells
- add samples to wells
- incubate for 1 hr or o/n
- wash with wash buffer
- add NFkB antibody and incubate for 1 hr , then wash
- add HRP-conjugated secondary antibody and incubate for 1 hr, then wash
- add developing solution and incubate for 15-45 min
- add stop solution
- analyze with microplate reader

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com