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Nile red staining kit ab228553 is designed to quantitatively measure intracellular lipid droplet accumulation in cell cultures.

- Suitable for fluorescent microplate reader, microscope and flow cytometry (Ex/Em 550/640 nm)
- Use for live cell or fixed cells

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Images

Fluorescence Microscopy - Nile Red Staining Kit (AB228553), expandable thumbnail

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Key facts

Detection method
Fluorescent
Sample types
Suspension cells, Adherent cells

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Target data

Alternative names

2410064E05Rik, AI195561, ATAD5_HUMAN, ATPase family AAA domain containing 5, ATPase family AAA domain-containing protein 5, BRCA1 DNA repair associated, BRCA1/BRCA2-containing complex, subunit 1, BRCA1_HUMAN, BRCAI, BRCC 1, BROVCA1, Breast Cancer 1, Breast Cancer 1 Early Onset, Breast and ovarian cancer susceptibility protein 1, Breast cancer type 1 susceptibility protein, C17orf41, Chromosome fragility-associated gene 1 protein, DmRab9, ELG1, Enhanced level of genomic instability 1 homolog, FANCS, FRAG1, G-protein coupled receptor 55, GPCR GPR55, GPR55_HUMAN, IRIS, LPIR1, NPC, NPC1_HUMAN, Niemann Pick C1 protein precursor, Niemann Pick disease, type C1, Niemann-Pick C1 protein, PNCA4, PPP1R53, PSCP, Probable G protein coupled receptor 55, Protein phosphatase 1 regulatory subunit 53, RAB9 member RAS oncogene family, RAB9A member RAS oncogene family, RAB9A_HUMAN, RAS ASSOCIATED PROTEIN RAB9, RING finger protein 53, RNF53, Rab 9, Ras-related protein Rab-9A, Sid6061p, Sid99, chromosome fragility associated gene 1

What's included?

200 Test
Components
Nile Red
1 x 40 µL
Staining Buffer
1 x 20 mL

Recommended products

Nile red staining kit ab228553 is designed to quantitatively measure intracellular lipid droplet accumulation in cell cultures.

- Suitable for fluorescent microplate reader, microscope and flow cytometry (Ex/Em 550/640 nm)
- Use for live cell or fixed cells

Key facts

Detection method
Fluorescent
Sample types
Suspension cells, Adherent cells
Assay Platform
Microplate reader, Fluor. microscope, Flow cyt.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Nile Red Staining Kit ab228553 is designed for the quantitative, fluorometric detection and measurement of intracellular lipid droplets using fluorescence microscopy, flow cytometry or a fluorescence microplate reader.

In the assay, Nile Red dye is added to the cell samples and the dye selectively stains lipid droplets within the cells. The dye is minimally fluorescent in solution in water so it is not necessary to wash the cells or remove the dye before analysis.

Excitation / emission: 550 / 640 nm.

Lipid droplets are also referred to as lipid bodies, oil bodies, and adiposomes.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Fluorescence Microscopy - Nile Red Staining Kit (ab228553), expandable thumbnail

    Fluorescence Microscopy - Nile Red Staining Kit (ab228553)

    Fluorescence images of intracellular lipid droplets in 3T3-L1 fibroblasts (left panel) and 3T3-L1 adipocyte cells (right panel) using ab228553 Nile Red Staining Kit. The fluorescence signal was measured using a fluorescence microscope with a TRITC filter.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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