AB228553

Nile Red Staining Kit

Name: Nile red staining kit (fluorescent) (ab228553) | Abcam
Brand: Abcam Limited
SKU: AB228553
Price: 285 USD
Availability: InStock

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(15 Publications)

Nile red staining kit ab228553 is designed to quantitatively measure intracellular lipid droplet accumulation in cell cultures.

- Suitable for fluorescent microplate reader, microscope and flow cytometry (Ex/Em 550/640 nm)
- Use for live cell or fixed cells

1 Images

Fluorescence Microscopy - Nile Red Staining Kit (AB228553)

Fluorescence Microscopy

Supplier Data

Fluorescence Microscopy - Nile Red Staining Kit (AB228553)

Fluorescence images of intracellular lipid droplets in 3T3-L1 fibroblasts (left panel) and 3T3-L1 adipocyte cells (right panel) using ab228553 Nile Red Staining Kit. The fluorescence signal was measured using a fluorescence microscope with a TRITC filter.

Key facts

Detection method

Fluorescent

Sample types

Suspension cells, Adherent cells

Assay Platform

Microplate reader, Fluor. microscope, Flow cyt.

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Product details

Nile Red Staining Kit ab228553 is designed for the quantitative, fluorometric detection and measurement of intracellular lipid droplets using fluorescence microscopy, flow cytometry or a fluorescence microplate reader.

In the assay, Nile Red dye is added to the cell samples and the dye selectively stains lipid droplets within the cells. The dye is minimally fluorescent in solution in water so it is not necessary to wash the cells or remove the dye before analysis.

Excitation / emission: 550 / 640 nm.

Lipid droplets are also referred to as lipid bodies, oil bodies, and adiposomes.

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What's included?

{ "values": { "200Test": { "sellingSize": "200 Test", "publicAssetCode":"ab228553-200Test", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"Staining Buffer", "number":"AB228553-CMP02", "productcode":"" }, { "size":"1 x 40 µL", "name":"Nile Red", "number":"AB228553-CMP01", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Product protocols

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Target data

See full target information Nile red

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Environmental technology & innovation 38: PubMed40575715

2025

Assessment of household settled dust via silicon nanomembrane analysis pipeline (SNAP).

Applications

Unspecified application

Species

Unspecified reactive species

Samantha S Romanick,Gregory Madejski,Garrett Cashion,Andrew J Berger,Alison Elder,James McGrath

Plants (Basel, Switzerland) 14: PubMed40219059

2025

Nettle Leaf Water Extracts for Hepatoprotection: Insights into Bioactivity and Mitochondrial Function.

Applications

Unspecified application

Species

Unspecified reactive species

Ruta Muceniece,Beatrise Luize Revina,Jorens Kviesis,Aris Jansons,Kirills Kopiks,Kaspars Jekabsons,Kristine Saleniece,Jana Namniece,Zane Grigale-Sorocina,Baiba Jansone

PLoS genetics 21:e1011617 PubMed40048486

2025

A novel role for Friend of GATA1 (FOG-1) in regulating cholesterol transport in murine erythropoiesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ioannis-Marios Roussis,David J Pearton,Umar Niazi,Grigorios Tsaknakis,Giorgio L Papadopoulos,Riley Cook,Mansoor Saqi,Jiannis Ragoussis,John Strouboulis

Cells 13: PubMed39120332

2024

Enhanced In Vitro Recapitulation of In Vivo Liver Regeneration by Co-Culturing Hepatocyte Organoids with Adipose-Derived Mesenchymal Stem Cells, Alleviating Steatosis and Apoptosis in Acute Alcoholic Liver Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Sun A Ock,Seo-Yeon Kim,Young-Im Kim,Won Seok Ju,Poongyeon Lee

Neurobiology of disease 199:106593 PubMed38971480

2024

Reducing the lipase LIPE in mutant α-synuclein mice improves Parkinson-like deficits and reveals sex differences in fatty acid metabolism.

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Unspecified application

Species

Unspecified reactive species

M A Adom,W N Hahn,T D McCaffery,T E Moors,X Zhang,P Svenningsson,D J Selkoe,S Fanning,S Nuber

Autophagy 20:2529-2539 PubMed38869076

2024

CCDC50 mediates the clearance of protein aggregates to prevent cellular proteotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Ye,Penghui Jia,Jiafan Miao,Yicheng Wang,Zibo Li,Yuxin Lin,Miao He,Shurui Liu,Bi-Rong Zheng,Junyu Wu,Ji'an Pan,Chun-Mei Li,Panpan Hou,Deyin Guo

Respiratory research 24:314 PubMed38098035

2023

CD36/Lyn kinase interactions within macrophages promotes pulmonary fibrosis in response to oxidized phospholipid.

Applications

Unspecified application

Species

Unspecified reactive species

Doyun Kwak,Patrick B Bradley,Natalia Subbotina,Song Ling,Seagal Teitz-Tennenbaum,John J Osterholzer,Thomas H Sisson,Kevin K Kim

ACS nano 16:7309-7322 PubMed35504018

2022

Peptide Amphiphile Supramolecular Nanofibers Designed to Target Abdominal Aortic Aneurysms.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin T Ledford,Adam W Akerman,Kui Sun,David C Gillis,Jenna M Weiss,Johnny Vang,Smaranda Willcox,Tristan D Clemons,Hiroaki Sai,Ruomeng Qiu,Mark R Karver,Jack D Griffith,Nick D Tsihlis,Samuel I Stupp,John S Ikonomidis,Melina R Kibbe

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 19:1018-1036 PubMed35445353

2022

A Brain-Penetrant Stearoyl-CoA Desaturase Inhibitor Reverses α-Synuclein Toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Silke Nuber,Chee Yeun Chung,Daniel F Tardiff,Pascal A Bechade,Thomas D McCaffery,Kazuma Shimanaka,Jeonghoon Choi,Belle Chang,Waseem Raja,Esther Neves,Christopher Burke,Xin Jiang,Ping Xu,Vikram Khurana,Ulf Dettmer,Saranna Fanning,Kenneth J Rhodes,Dennis J Selkoe,Robert H Scannevin

Cell research 32:54-71 PubMed34561619

2021

Nuclear UHRF1 is a gate-keeper of cellular AMPK activity and function.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Xu,Guangjin Ding,Caizhi Liu,Yuhan Ding,Xiaoxin Chen,Xiaoli Huang,Chen-Song Zhang,Shanxin Lu,Yunpeng Zhang,Yuanyong Huang,Zhaosu Chen,Wei Wei,Lujian Liao,Shu-Hai Lin,Jingya Li,Wei Liu,Jiwen Li,Sheng-Cai Lin,Xinran Ma,Jiemin Wong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Nile Red Staining Kit

Fluorescence Microscopy - Nile Red Staining Kit (AB228553)

Key facts

Detection method

Sample types

Assay Platform

Complementary Products

Secondary Antibodies

Primary Antibodies

Assay Kits

Biochemicals

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (15)

Product promise