NIR Mitochondrial Membrane Potential Assay Kit (Flow Cytometry)

NIR Mitochondrial Membrane Potential Assay Kit (Flow Cytometry) ab112149 is designed to detect cell apoptosis by measuring the loss of the mitochondrial membrane potential. The collapse of mitochondrial membrane potential coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome C into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.

ab112149 uses our proprietary cationic NIR probe for the detection of apoptosis in cells with the loss of mitochondrial membrane potential. In normal cells, the red fluorescence intensity is increased when the MitoNIR Dye is accumulated in the mitochondria. However, in apoptotic cells, the NIR stain intensity is decreased following the collapse of MMP. Cells stained with NIR Dye can be visualized with a flow cytometer at red excitation and far red emission (FL4 channel).

ab112149 provides all the essential components. ab112149 can be used together with other reagents, such as blue laser excited propidium iodide and for studying cell vitality and apoptosis. ab112149 is optimized for screening apoptosis activators and inhibitors with a flow cytometer.

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