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AB65328

Nitric Oxide Assay Kit (Colorimetric)

4

(2 Reviews)

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(133 Publications)

Nitric Oxide Assay Kit (Colorimetric) ab65328 uses nitrate reductase and the Griess reagent method. It can be used to quantify both total nitrate/nitrite levels and/or nitrate and nitrite levels separately. Readout on any colorimetric (540 nm) plate reader.

- Cited in >120 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
6 Images
Functional Studies - Nitric Oxide Assay Kit (Colorimetric) (AB65328)
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Lab

Functional Studies - Nitric Oxide Assay Kit (Colorimetric) (AB65328)

Nitrite and nitrate measured in biologicals showing concentration (micromolar)

Functional Studies - Nitric Oxide Assay Kit (Colorimetric) (AB65328)
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Lab

Functional Studies - Nitric Oxide Assay Kit (Colorimetric) (AB65328)

Nitrite and nitrate measured in cell lysates showing quantity (micromol) per 106 cells

Functional Studies - Nitric Oxide Assay Kit (Colorimetric) (AB65328)
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Functional Studies - Nitric Oxide Assay Kit (Colorimetric) (AB65328)

Blood NOx concentration measured with ab65328. Blood NOx concentration after application of the NE with NO. The level of blood NOx concentration increased 3-fold after the application of the NE with NO compared with control group.

Nam, Eunryel et al., Scientific reports vol. 8,1 7312., Fig 4 , doi:10.1038/s41598-018-25786-x

Functional Studies - Nitric Oxide Assay Kit (Colorimetric) (AB65328)
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Lab

Functional Studies - Nitric Oxide Assay Kit (Colorimetric) (AB65328)

Standard curve (colorimetric) : mean of duplicates (+/-SD) with background readings subtracted

Functional Studies - Nitric Oxide Assay Kit (Colorimetric) (AB65328)
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Lab

Functional Studies - Nitric Oxide Assay Kit (Colorimetric) (AB65328)

Standard curve (Colorimetric) : mean of duplicates (+/-SD) with background readings subtracted

Schematic Diagram - Nitric Oxide Assay Kit (Colorimetric) (AB65328)
  • Schematic Diagram

Supplier Data

Schematic Diagram - Nitric Oxide Assay Kit (Colorimetric) (AB65328)

Representative image of Nitric Oxide Assay Kit (Colorimetric) ab65328

Components shown from left to right :

- Enzyme Cofactor

- Enhancer II

- Nitrate Standard

- Nitrite Standard

- Griess Reagent I

- Griess Reagent II

- Assay Buffer 17

- AlumaSeal 96 Film (2 films)

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Tissue Extracts, Serum, Other biological fluids, Cell Lysate

Assay type

Quantitative

Sensitivity

= 1 nmol/well

Range

1 - 10 nmol/well

Assay time

1h 30m

Assay Platform

Microplate reader

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Product details

Nitric Oxide Assay Kit (Colorimetric) ab65328 provides an accurate, convenient measure of total nitrate/nitrite in a simple two-step process.

Nitric oxide assay principle
- the first step converts nitrate to nitrite by using nitrate reductase
- the second step uses Griess Reagent to convert nitrite to a deep purple azo compound (540 nm).
The amount of the azochromophore accurately reflects the nitric oxide amount in samples.
The assay measures total nitrate + nitrite levels. To measure nitrate and nitrite levels separately, samples can also be run without the nitrate reductase, to measure nitrite, and nitrate levels are calculated by subtracting nitrite levels from total nitrate + nitrite.

Nitric oxide assay protocol summary
- add samples and standards to wells
- add nitrate reductase and enzyme cofactor and incubate for 1 hr at room temp to convert nitrate to nitrite
- add enhancer and incubate for 10 min at room temp
- add Griess Reagent R1 and Griess Reagent R2
- analyze with microplate reader

Related and recommended products
If you are interested in a fluorometric detection kit, please check Nitric Oxide Assay Kit (Fluorometric) ab65327.
For an assay kit to detect nitrite alone see Griess Reagent Kit ab234044.

Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. NO is rapidly oxidized to nitrite and nitrate which are used to quantitate NO production.

This product was previously called K262 Biovision Nitric Oxide Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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What's included?

{ "values": { "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab65328-2x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Nitrite Standard", "number":"AB65328-CMP09", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Nitrate Standard", "number":"AB65328-CMP08", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Nitrate Reductase Enzyme", "number":"AB65328-CMP07", "productcode":"" }, { "size":"2 x 1 Unit", "name":"Microplate Sealing Film", "number":"AB65328-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"Griess Reagent II", "number":"AB65328-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"Griess Reagent I", "number":"AB65328-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Enzyme Cofactor", "number":"AB65328-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Enhancer II", "number":"AB65328-CMP02", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Buffer 17", "number":"AB65328-CMP01", "productcode":"AB323985" }, { "size":"2 x 1 Unit", "name":"96-Well Clear Plate", "number":"AB65328-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nitric oxide (NO) is a small diatomic molecule with a mass of 30.01 Da. It plays a significant mechanical role as a signaling molecule in numerous systems. Nitric oxide is synthesized by nitric oxide synthases (NOS) and is expressed in endothelial cells neurons and various immune cells. Its production occurs in response to mechanical shear stress calcium influx or immunological stimulation. Nitric oxide exhibits high diffusibility enabling it to cross cellular membranes easily which is key for its function as a signaling messenger.
Biological function summary

Nitric oxide mediates vasodilation neurotransmission and immune response modulation. It is important for maintaining vascular homeostasis where it relaxes smooth muscle tissue promoting blood flow. In the central nervous system nitric oxide acts as a neurotransmitter and neuromodulator influencing synaptic plasticity. Within the immune system it functions to regulate leukocyte adhesion and can mediate microbial destruction. Nitric oxide often works within complexes associating with proteins like guanylate cyclase enhancing its signaling efficacy through nitrosylation.

Pathways

Nitric oxide integrates into key biological processes such as the nitric oxide signaling pathway and the endothelial nitric oxide pathway. It activates soluble guanylate cyclase increasing cyclic GMP levels leading to smooth muscle relaxation and vasodilation. In the endothelial pathway nitric oxide links with other proteins like endothelium-derived relaxing factor and interacts with oxidative stress pathways showing its role in cellular defense mechanisms. These pathways manage critical physiological responses and connect nitric oxide to other cardiovascular regulators.

Nitric oxide dysregulation associates with conditions like cardiovascular disease and neurodegenerative disorders. A deficiency or excess production can contribute to hypertension or atherosclerosis given its role in vascular health. Neurodegenerative diseases such as Alzheimer's disease also show links to nitric oxide largely due to its involvement in neuroinflammation and synaptic dysfunctions. It interacts with proteins like amyloid-beta precursor protein and tau in these contexts highlighting its importance in pathology and therapeutic targets.
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Product protocols

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Target data

See full target information Nitric Oxide
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Publications (133)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39457062

2024

The Interplay of Nitric Oxide and Nitrosative Modifications in Maize: Implications for Aphid Herbivory and Drought Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Hubert Sytykiewicz,Paweł Czerniewicz,Magdalena Ruszczyńska,Katarzyna Kmieć

Cell communication and signaling : CCS 22:452 PubMed39327567

2024

Non-thermal atmospheric pressure plasma induces selective cancer cell apoptosis by modulating redox homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Ju Hyun Yun,Yoon Hee Yang,Chang Hak Han,Sung Un Kang,Chul-Ho Kim

Chemistry & biodiversity 21:e202400959 PubMed39077790

2024

In-Vitro Cytotoxic and Anti-Inflammatory Potential of Asparagus Densiflorus Meyeri and its Phytochemical Investigation.

Applications

Unspecified application

Species

Unspecified reactive species

Yasmin Sobhy,Shahenda Mahgoub,Yasmin Abo-Zeid,Suzan A Mina,Mohamed S Mady

Healthcare (Basel, Switzerland) 12: PubMed38921341

2024

Toloese Generates Nitric Oxide through Natural Radiation of Far Infrared Rays, Reducing Serum Glucose, Cholesterol, and Triglycerides.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Ho Yeo,Young-Hyeon Lee,Mi-Jin Ryu,Yong-Hak Choi,Hye-Sook Kim,Kyung-Soo Chang

Frontiers in pharmacology 15:1389670 PubMed38910880

2024

Sitagliptin ameliorates L-arginine-induced acute pancreatitis via modulating inflammatory cytokines expression and combating oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Heba M Eltahir,Hossein M Elbadawy,Mohannad A Almikhlafi,Ali M Alalawi,Ahmed J Aldhafiri,Yaser M Alahmadi,Sultan S Al Thagfan,Muayad Albadrani,Saber M Eweda,Mekky M Abouzied

Heliyon 10:e30808 PubMed38818176

2024

Tumor cell-derived exosomal miR-193b-3p promotes tumor-associated macrophage activation to facilitate nasopharyngeal cancer cell invasion and radioresistances.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Li,Xing Xing,Chunying Shen,Chaosu Hu

Hypertension research : official journal of the Japanese Society of Hypertension 47:1627-1641 PubMed38605139

2024

The soluble (pro)renin receptor promotes a preeclampsia-like phenotype both in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Lachlan G Schofield,Sarah J Delforce,Jennifer C Pryor,Saije K Endacott,Eugenie R Lumbers,Sarah A Marshall,Kirsty G Pringle

Clinical science (London, England : 1979) 138:251-268 PubMed38362910

2024

Inhibition of the histone methyltransferase EZH2 induces vascular stiffness.

Applications

Unspecified application

Species

Unspecified reactive species

Jaime Ibarrola,Rachel R Xiang,Zhe Sun,Qing Lu,Michael A Hill,Iris Z Jaffe

International journal of molecular sciences 25: PubMed38255863

2024

The Use of Nitrosative Stress Molecules as Potential Diagnostic Biomarkers in Multiple Sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Saskia Räuber,Moritz Förster,Julia Schüller,Alice Willison,Kristin S Golombeck,Christina B Schroeter,Menekse Oeztuerk,Robin Jansen,Niklas Huntemann,Christopher Nelke,Melanie Korsen,Katinka Fischer,Ruth Kerkhoff,Yana Leven,Patricia Kirschner,Tristan Kölsche,Petyo Nikolov,Mohammed Mehsin,Gelenar Marae,Alma Kokott,Duygu Pul,Julius Schulten,Niklas Vogel,Jens Ingwersen,Tobias Ruck,Marc Pawlitzki,Sven G Meuth,Nico Melzer,David Kremer

Nature communications 14:7639 PubMed37993431

2023

Acoustofluidic Interfaces for the Mechanobiological Secretome of MSCs.

Applications

Unspecified application

Species

Unspecified reactive species

Ye He,Shujie Yang,Pengzhan Liu,Ke Li,Ke Jin,Ryan Becker,Jinxin Zhang,Chuanchuan Lin,Jianping Xia,Zhehan Ma,Zhiteng Ma,Ruoyu Zhong,Luke P Lee,Tony Jun Huang
View all publications
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