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Nitric Oxide Assay Kit (Colorimetric) ab65328 provides an accurate, convenient measure of total nitrate/nitrite in a simple two-step process.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.


Images

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Tissue Extracts, Serum, Other biological fluids, Cell Lysate

Assay type

Quantitative

Reactive species

Mammals

Range

1 - 10 nmol/well

Assay time

1h 30m

Sensitivity

= 1 nmol/well

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What's included?

2 x 96 Tests
Components
96-Well Clear Plate
2 x 1 Unit
Assay Buffer XVII
1 x 30 mL
Enhancer II
1 x 1 Vial
Enzyme Cofactor
1 x 1 Vial
Griess Reagent I
1 x 10 mL
Griess Reagent II
1 x 10 mL
Microplate Sealing Film
2 x 1 Unit
Nitrate Reductase I
1 x 1 Vial
Nitrate Standard
1 x 1 Vial
Nitrite Standard
1 x 1 Vial

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Nitric Oxide Assay Kit (Colorimetric) ab65328 provides an accurate, convenient measure of total nitrate/nitrite in a simple two-step process.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Tissue Extracts, Serum, Other biological fluids, Cell Lysate

Assay type

Quantitative

Reactive species

Mammals

Range

1 - 10 nmol/well

Assay time

1h 30m

Assay Platform

Microplate reader

Sensitivity

= 1 nmol/well

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Nitric Oxide Assay Kit (Colorimetric) ab65328 provides an accurate, convenient measure of total nitrate/nitrite in a simple two-step process.

In the nitric oxide assay protocol:
- the first step converts nitrate to nitrite by using nitrate reductase
- the second step uses Griess Reagent to convert nitrite to a deep purple azo compound.
The amount of the azochromophore accurately reflects nitric oxide amount in samples.

Nitric oxide assay protocol summary:
- add samples and standards to wells
- add nitrate reductase and enzyme cofactor and incubate for 1 hr at room temp to convert nitrate to nitrite
- add enhancer and incubate for 10 min at room temp
- add Griess Reagent R1 and Griess Reagent R2
- analyze with microplate reader

This product is manufactured by BioVision, an Abcam company and was previously called K262 Nitric Oxide Colorimetric Assay Kit. K262-200 is the same size as the 2 96 tests size of ab65328.

If you are interested in a fluorometric detection kit, please check Nitric Oxide Assay Kit (Fluorometric) (ab65327).

For an assay kit to detect nitrite alone see Griess Reagent Kit ab234044.

Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. NO is rapidly oxidized to nitrite and nitrate which are used to quantitate NO production.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Functional Studies - Nitric Oxide Assay Kit (Colorimetric) (ab65328), expandable thumbnail
    Nam, Eunryel et al., Scientific reports vol. 8,1 7312., Fig 4 , doi:10.1038/s41598-018-25786-x

    Functional Studies - Nitric Oxide Assay Kit (Colorimetric) (ab65328)

    Blood NOx concentration measured with ab65328. Blood NOx concentration after application of the NE with NO. The level of blood NOx concentration increased 3-fold after the application of the NE with NO compared with control group.

  • Functional Studies - Nitric Oxide Assay Kit (Colorimetric) (ab65328), expandable thumbnail

    Functional Studies - Nitric Oxide Assay Kit (Colorimetric) (ab65328)

    Nitrite and nitrate measured in cell lysates showing quantity (micromol) per 106 cells

  • Functional Studies - Nitric Oxide Assay Kit (Colorimetric) (ab65328), expandable thumbnail

    Functional Studies - Nitric Oxide Assay Kit (Colorimetric) (ab65328)

    Nitrite and nitrate measured in biologicals showing concentration (micromolar)

  • Functional Studies - Nitric Oxide Assay Kit (Colorimetric) (ab65328), expandable thumbnail

    Functional Studies - Nitric Oxide Assay Kit (Colorimetric) (ab65328)

    Standard curve (colorimetric) : mean of duplicates (+/-SD) with background readings subtracted

  • Functional Studies - Nitric Oxide Assay Kit (Colorimetric) (ab65328), expandable thumbnail

    Functional Studies - Nitric Oxide Assay Kit (Colorimetric) (ab65328)

    Standard curve (Colorimetric) : mean of duplicates (+/-SD) with background readings subtracted

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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