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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Nitric Oxide Assay Kit (Colorimetric) ab65328 provides an accurate, convenient measure of total nitrate/nitrite in a simple two-step process.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.
Colorimetric
Urine, Plasma, Tissue Extracts, Serum, Other biological fluids, Cell Lysate
Quantitative
Mammals
1 - 10 nmol/well
1h 30m
= 1 nmol/well
Nitric Oxide Assay Kit (Colorimetric) ab65328 provides an accurate, convenient measure of total nitrate/nitrite in a simple two-step process.
Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.
Colorimetric
Urine, Plasma, Tissue Extracts, Serum, Other biological fluids, Cell Lysate
Quantitative
Mammals
1 - 10 nmol/well
1h 30m
Microplate reader
= 1 nmol/well
Blue Ice
-20°C
-20°C
-20°C
Nitric Oxide Assay Kit (Colorimetric) ab65328 provides an accurate, convenient measure of total nitrate/nitrite in a simple two-step process.
In the nitric oxide assay protocol:
- the first step converts nitrate to nitrite by using nitrate reductase
- the second step uses Griess Reagent to convert nitrite to a deep purple azo compound.
The amount of the azochromophore accurately reflects nitric oxide amount in samples.
Nitric oxide assay protocol summary:
- add samples and standards to wells
- add nitrate reductase and enzyme cofactor and incubate for 1 hr at room temp to convert nitrate to nitrite
- add enhancer and incubate for 10 min at room temp
- add Griess Reagent R1 and Griess Reagent R2
- analyze with microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K262 Nitric Oxide Colorimetric Assay Kit. K262-200 is the same size as the 2 96 tests size of ab65328.
If you are interested in a fluorometric detection kit, please check Nitric Oxide Assay Kit (Fluorometric) (ab65327).
For an assay kit to detect nitrite alone see Griess Reagent Kit ab234044.
Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. NO is rapidly oxidized to nitrite and nitrate which are used to quantitate NO production.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blood NOx concentration measured with ab65328. Blood NOx concentration after application of the NE with NO. The level of blood NOx concentration increased 3-fold after the application of the NE with NO compared with control group.
Nitrite and nitrate measured in cell lysates showing quantity (micromol) per 106 cells
Nitrite and nitrate measured in biologicals showing concentration (micromolar)
Standard curve (colorimetric) : mean of duplicates (+/-SD) with background readings subtracted
Standard curve (Colorimetric) : mean of duplicates (+/-SD) with background readings subtracted
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com